The specific neuroendocrine profile is critically involved in an adequate T-dependent immune response

A functional relationship between the neuroendocrine and immune systems has been clearly established. We examined the role of neuroendocrine changes, particularly thyrotropin-releasing hormone (TRH) and prolactin (PRL), during the T cell-dependent immune response. After immunization of rats with she...

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Detalles Bibliográficos
Autores principales: Castro, C.P., Pereda, M.P., Keller, E.A., Arzt, E.
Formato: JOUR
Materias:
Immune modulation
Prolactin
SRBC
T-lymphocytes
TRH antisense
antisense oligonucleotide
protirelin
analysis of variance
animal
antibody production
article
blood
erythrocyte
immune system
immunology
male
metabolism
neurosecretion
rat
sheep
T lymphocyte
Wistar rat
Analysis of Variance
Animals
Antibody Formation
Erythrocytes
Immune System
Male
Neurosecretory Systems
Oligonucleotides, Antisense
Rats
Rats, Wistar
Sheep
T-Lymphocytes
Thyrotropin-Releasing Hormone
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_00257680_v58_n2_p189_Castro
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
Descripción
Sumario:A functional relationship between the neuroendocrine and immune systems has been clearly established. We examined the role of neuroendocrine changes, particularly thyrotropin-releasing hormone (TRH) and prolactin (PRL), during the T cell-dependent immune response. After immunization of rats with sheep red blood cells (SRBC, a T cell-dependent antigen) we observed: a) an increase of hypothalamic TRH mRNA at 4 to 24 h post-immunization (i.e: SRBC vs saline: 4 h, 2.8x), in contrast to the decrease of TRH mRNA observed following treatment with LPS, a T-independent antigen (LPS vs saline: 4 h, 1.6x); b) an increase in pituitary TRH receptor mRNA and plasma PRL levels but no changes in thyroid-stimulating hormone and growth hormone plasma levels. Intracerebroventricular (icv) injection in conscious freely-moving rats of antisense oligonucleotide complementary to rat TRH mRNA resulted in: a) a significant inhibition of specific antibody production [ELISA 7 days: Ig(M+G): TRH sense vs TRH-antisense: 384 ± 27 (n = 11) vs 193 ± 22 (n = 11); p < 0.001] b) an inability to produce the peak in plasma PRL levels in rats immunized with SRBC [(12h post-immunization, TRH-sense vs TRH-antisense: 8.3 ± 1.4 (n = 6) vs 2.2 ± 0.5 (n = 6); p < 0 01]; c) a decrease in hypothalamic TRH mRNA (TRH-sense vs TRH-antisense: 12h, 1.7x). These studies demonstrate that the T-cell antigen needs an early activation of TRH and PRL for an adequate immune response, in contrast to the inhibition induced by a T-cell independent antigen.

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