Characteristics of an inulinase produced by Bacillus subtilis 430A, a strain isolated from the rhizosphere of Vernonia herbacea (Vell Rusby)

Bacillus subtilis 430A, isolated from the Vernonia herbacea (Vell Rusby) rhizosphere, produced an exocellular inulinase that fits the requirements for the production of syrups on an industrial scale. The partially purified enzyme, obtained by acetone precipitation, displayed a higher specificity for...

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Detalles Bibliográficos
Autores principales: Vullo, D.L., Coto, C.E., Sineriz, F.
Formato: JOUR
Materias:
inulin
inulinase
article
bacillus subtilis
biosynthesis
enzyme analysis
enzyme purification
nonhuman
priority journal
temperature
Bacillus subtilis
Enzyme Stability
Insulysin
Plants
Soil Microbiology
Substrate Specificity
Support, Non-U.S. Gov't
Temperature
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_00992240_v57_n8_p2392_Vullo
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
Descripción
Sumario:Bacillus subtilis 430A, isolated from the Vernonia herbacea (Vell Rusby) rhizosphere, produced an exocellular inulinase that fits the requirements for the production of syrups on an industrial scale. The partially purified enzyme, obtained by acetone precipitation, displayed a higher specificity for inulin (K(m), 8 mM) than for sucrose (56 mM) and a total invertase/total inulase ratio of 0.62. In addition, it is stable at an optimal temperature of 45 to 50°C for at least 7 h and is inhibited by the end product, fructose, at 14 mM.

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