Site-specific recombination system from Tn 1000(γδ) stabilises recombinant plasmids in Escherichi coli
The resolution system from Tn 1000 was cloned into a pBR322 recombinant plasmid carrying E. coli threonine operon. The resulting plasmid displayed a significant increase in segregational stability with respect to the original plasmid, either in a rec+ or a rec- E. coli strain (100% of plasmid-contai...
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| Autores principales: | , , |
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| Formato: | JOUR |
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_01415492_v19_n4_p331_Bellani |
| Aporte de: |
| Sumario: | The resolution system from Tn 1000 was cloned into a pBR322 recombinant plasmid carrying E. coli threonine operon. The resulting plasmid displayed a significant increase in segregational stability with respect to the original plasmid, either in a rec+ or a rec- E. coli strain (100% of plasmid-containing cells versus 64% and 1% respectively). The Tn 1000 resolution system proved to be responsible for the stabilising effect exerted by the transposon when present in recombinant plasmids. |
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