S-layer proteins from Lactobacillus sp. inhibit bacterial infection by blockage of DC-SIGN cell receptor

Many species of Lactobacillus sp. possess Surface(s) layer proteins in their envelope. Among other important characteristics S-layer from Lactobacillus acidophilus binds to the cellular receptor DC-SIGN (Dendritic Cell-Specific Intercellular adhesion molecule-3-Grabbing Non-integrin; CD209), which i...

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Detalles Bibliográficos
Autores principales: Prado Acosta, M., Ruzal, S.M., Cordo, S.M.
Formato: JOUR
Materias:
DC-SIGN
Lactobacillus
S-layer
bacterial protein
CD209 antigen
s layer protein
unclassified drug
antiinfective agent
cell adhesion molecule
cell surface receptor
DC-specific ICAM-3 grabbing nonintegrin
lectin
membrane protein
S-layer proteins
Article
bacterial strain
bacterial viability
bacterium adherence
controlled study
enzyme inhibition
human
human cell
in vivo study
Lactobacillus acidophilus
Lactobacillus brevis
Lactobacillus helveticus
Lactobacillus kefiri
nonhuman
protein function
protein glycosylation
protein protein interaction
3T3 cell line
animal
Bacterial Infections
chemistry
drug effects
glycosylation
metabolism
mouse
3T3 Cells
Animals
Anti-Bacterial Agents
Bacterial Adhesion
Cell Adhesion Molecules
Glycosylation
Humans
Lectins, C-Type
Membrane Glycoproteins
Mice
Receptors, Cell Surface
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_01418130_v92_n_p998_PradoAcosta
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
Descripción
Sumario:Many species of Lactobacillus sp. possess Surface(s) layer proteins in their envelope. Among other important characteristics S-layer from Lactobacillus acidophilus binds to the cellular receptor DC-SIGN (Dendritic Cell-Specific Intercellular adhesion molecule-3-Grabbing Non-integrin; CD209), which is involved in adhesion and infection of several families of bacteria. In this report we investigate the activity of new S-layer proteins from the Lactobacillus family (Lactobacillus acidophilus, Lactobacillus brevis, Lactobacillus helveticus and Lactobacillus kefiri) over the infection of representative microorganisms important to human health. After the treatment of DC-SIGN expressing cells with these proteins, we were able to diminish bacterial infection by up to 79% in both gram negative and mycobacterial models. We discovered that pre-treatment of the bacteria with S-layers from Lactobacillus acidophilus and Lactobacillus brevis reduced bacteria viability but also prevent infection by the pathogenic bacteria. We also proved the importance of the glycosylation of the S-layer from Lactobacillus kefiri in the binding to the receptor and thus inhibition of infection. This novel characteristic of the S-layers proteins may contribute to the already reported pathogen exclusion activity for these Lactobacillus probiotic strains; and might be also considered as a novel enzymatic antimicrobial agents to inhibit bacterial infection and entry to host cells. © 2016 Elsevier B.V.

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