High molecular weight components containing N-linked oligosaccharides of Ascaris suum extract inhibit the dendritic cells activation through DC-SIGN and MR

Helminths, as well as their secretory/excretory products, induce a tolerogenic immune microenvironment. High molecular weight components (PI) from Ascaris suum extract down-modulate the immune response against ovalbumin (OVA). The PI exerts direct effect on dendritic cells (DCs) independent of TLR 2...

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Detalles Bibliográficos
Autores principales: Favoretto, B.C., Casabuono, A.A.C., Portes-Junior, J.A., Jacysyn, J.F., Couto, A.S., Faquim-Mauro, E.L.
Formato: JOUR
Materias:
Ascaris suum
C-type lectin receptors
DC-Dendritic Cell
DCSIGN-Dendritic cell specifc ICAM-3 grabbing non-integrin
MR-Mannose receptor
PI - High molecular weight components from Ascaris suum extract
asparagine linked oligosaccharide
CD209 antigen
Freund adjuvant
granulocyte macrophage colony stimulating factor
high molecular weight component
interleukin 4
mannan
mannose receptor
natural products and their synthetic derivatives
ovalbumin
unclassified drug
lectin
oligosaccharide
phosphorylcholine
animal cell
animal experiment
Article
binding affinity
binding assay
bound fraction
Canavalia ensiformis
cell activation
cell surface
cell suspension
confocal microscopy
dendritic cell
flow cytometry
immunization
in vitro study
internalization
lectin binding
lymphocyte proliferation
male
matrix assisted laser desorption ionization time of flight mass spectrometry
molecular weight
mouse
nonhuman
priority journal
protein content
purification
retention time
T lymphocyte
animal
Bagg albino mouse
cell proliferation
chemistry
drug effects
immunology
lymphocyte activation
Animals
Cell Proliferation
Dendritic Cells
Lectins, C-Type
Lymphocyte Activation
Male
Mannans
Mice
Mice, Inbred BALB C
Molecular Weight
Oligosaccharides
Ovalbumin
Phosphorylcholine
T-Lymphocytes
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_01615890_v87_n_p33_Favoretto
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
Descripción
Sumario:Helminths, as well as their secretory/excretory products, induce a tolerogenic immune microenvironment. High molecular weight components (PI) from Ascaris suum extract down-modulate the immune response against ovalbumin (OVA). The PI exerts direct effect on dendritic cells (DCs) independent of TLR 2, 4 and MyD88 molecule and, thus, decreases the T lymphocytes response. Here, we studied the glycoconjugates in PI and the role of C-type lectin receptors (CLRs), DC-SIGN and MR, in the modulation of DCs activity. Our data showed the presence of glycoconjugates with high mannose- and complex-type N-linked oligosaccharide chains and phosphorylcholine residues on PI. In addition, these N-linked glycoconjugates inhibited the DCs maturation induced by LPS. The binding and internalization of PI-Alexa were decreased on DCs previously incubated with mannan, anti-DC-SIGN and/or anti-MR antibodies. In agreement with this, the incubation of DCs with mannan, anti-DC-SIGN and/or anti-MR antibodies abolished the down-modulatory effect of PI on these cells. It was also observed that the blockage of CLRs, DC-SIGN and MR on DCs reverted the inhibitory effect of PI in in vitro T cells proliferation. Therefore, our data show the involvement of DC-SIGN and MR in the recognition and consequent modulatory effect of N-glycosylated components of PI on DCs. © 2017 Elsevier Ltd

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