Adenylate cyclase activity in Cyanobacteria: activation by Ca2+-calmodulin and a calmodulin-like activity

An adenylate cyclase activity was partially characterized in the cyanobacterium Anabaena sp. The enzyme activity is found in soluble cell fractions and shows an apparent molecular weight of about 183 400. This adenylate cyclase is activated by Ca2+ and bovine brain or spinach calmodulin and it is in...

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Detalles Bibliográficos
Autores principales: Bianchini, G.M., Pastini, A.C., Muschietti, J.P., Téllez-Iñón, M.T., Martinetto, H.E., Torres, H.N., Flawiá, M.M.
Formato: JOUR
Materias:
(Anabaena)
(Cyanobacteria)
Adenylate cyclase
Calmodulin
Calmodulin-like activity
cyclic AMP
adenylate cyclase
calmodulin
chlorpromazine
egtazic acid
phenothiazine derivative
trifluoperazine
anabaena
article
nonhuman
priority journal
Adenylate Cyclase
Calcium
Cyanobacteria
Enzyme Activation
Support, Non-U.S. Gov't
Anabaena
Anabaena sp.
Bovinae
Spinacia oleracea
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_01674889_v1055_n1_p75_Bianchini
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
Descripción
Sumario:An adenylate cyclase activity was partially characterized in the cyanobacterium Anabaena sp. The enzyme activity is found in soluble cell fractions and shows an apparent molecular weight of about 183 400. This adenylate cyclase is activated by Ca2+ and bovine brain or spinach calmodulin and it is inhibited by EGTA and some phenothiazine derivatives. Furthermore, Anabaena sp. extracts contain a calmodulin-like activity which stimulates bovine brain cyclic AMP phosphodiesterase and the Anabaena adenylate cyclase. EGTA and phenothiazine derivatives block the cyano-bacterial modulator effect. © 1990.

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