Characterization and distribution of Citrobacter strains in a University Hospital
OBJETIVE. a) To identify Citrobacter strains following the conventional biochemical reaction of Brenner and col; b) to evaluate the sensitivity and specificity of the O'Hara's method compared with Brenner's method, and c) to determine the rate and distribution of the strains in the cl...
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| Autores principales: | , , , , , , , |
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| Formato: | JOUR |
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_0213005X_v19_n1_p11_Manganello |
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| Sumario: | OBJETIVE. a) To identify Citrobacter strains following the conventional biochemical reaction of Brenner and col; b) to evaluate the sensitivity and specificity of the O'Hara's method compared with Brenner's method, and c) to determine the rate and distribution of the strains in the clinical isolates. MATERIAL AND METHODS. One hundred and twenty two clinical isolates, characterized as Citrobacter spp. were collected between May of 1994 and August of 1997. Clinical isolates included impatients and outpatients from Hospital de Clinicas. Strains were identified following the methods of Brenner and O'Hara. RESULTS. Methods of Brenner identified 111 of 122 strains: C. freundii 59 of 111; C. koseri 18 of 111; C. werkmanii 15 of 111; C. braakii 9 of 111; C. youngae 6 of 111 and C. amalonaticus 4 of 111. O'Hara's methods identified 104 of 111 strains (94%). C. freundii was recovered most frequently from urine and feces (p Fisher <0.026 and 0.039 respectively), while C. koseri was isolated from urine principally (p Fisher <0.0372). CONCLUSIONS. The genus Citrobacter is and important oportunistic pathogen that can be identified in clinical microbiology's laboratories using O'Hara's method. |
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