A single-step purification of an extracellular fungal laccase

A single-step purification procedure for Neurospora crassa laccase is reported. It used Celite chromatography which permits the concentration of the extracellular enzyme from large culture volumes. This method is useful to isolate any fungal or plant laccase as well as other coppercontaining protein...

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Detalles Bibliográficos
Autores principales: Grotewold, E., Taccioli, G.E., Aisemberg, G.O., Judewicz, N.D.
Formato: JOUR
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_02650762_v4_n3_p357_Grotewold
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_02650762_v4_n3_p357_Grotewold
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spelling todo:paper_02650762_v4_n3_p357_Grotewold2023-10-03T15:13:20Z A single-step purification of an extracellular fungal laccase Grotewold, E. Taccioli, G.E. Aisemberg, G.O. Judewicz, N.D. A single-step purification procedure for Neurospora crassa laccase is reported. It used Celite chromatography which permits the concentration of the extracellular enzyme from large culture volumes. This method is useful to isolate any fungal or plant laccase as well as other coppercontaining proteins. This work also takes advantage of the 100-fold induction of the enzyme by low concentrations of cycloheximide. © 1988 Oxford University Press. Fil:Grotewold, E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Taccioli, G.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Aisemberg, G.O. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Judewicz, N.D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_02650762_v4_n3_p357_Grotewold
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
description A single-step purification procedure for Neurospora crassa laccase is reported. It used Celite chromatography which permits the concentration of the extracellular enzyme from large culture volumes. This method is useful to isolate any fungal or plant laccase as well as other coppercontaining proteins. This work also takes advantage of the 100-fold induction of the enzyme by low concentrations of cycloheximide. © 1988 Oxford University Press.
format JOUR
author Grotewold, E.
Taccioli, G.E.
Aisemberg, G.O.
Judewicz, N.D.
spellingShingle Grotewold, E.
Taccioli, G.E.
Aisemberg, G.O.
Judewicz, N.D.
A single-step purification of an extracellular fungal laccase
author_facet Grotewold, E.
Taccioli, G.E.
Aisemberg, G.O.
Judewicz, N.D.
author_sort Grotewold, E.
title A single-step purification of an extracellular fungal laccase
title_short A single-step purification of an extracellular fungal laccase
title_full A single-step purification of an extracellular fungal laccase
title_fullStr A single-step purification of an extracellular fungal laccase
title_full_unstemmed A single-step purification of an extracellular fungal laccase
title_sort single-step purification of an extracellular fungal laccase
url http://hdl.handle.net/20.500.12110/paper_02650762_v4_n3_p357_Grotewold
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AT judewicznd asinglesteppurificationofanextracellularfungallaccase
AT grotewolde singlesteppurificationofanextracellularfungallaccase
AT tacciolige singlesteppurificationofanextracellularfungallaccase
AT aisemberggo singlesteppurificationofanextracellularfungallaccase
AT judewicznd singlesteppurificationofanextracellularfungallaccase
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