Expresión de receptores de membrana plaquetaria en el sindrome urémico hemolîtico
The aim of the present study was to determine platelet membrane receptors in the Hemolytic Uremic Syndrome (HUS). 20 children of 17.3 ± 5.2 months of age, with clinical and laboratory diagnosis of HUS were studied. Platelets from samples obtained 15 days, one month and two months after the beginning...
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| Autores principales: | , , , , |
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| Formato: | JOUR |
| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_03252957_v33_n2_p197_Zanaro |
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| Sumario: | The aim of the present study was to determine platelet membrane receptors in the Hemolytic Uremic Syndrome (HUS). 20 children of 17.3 ± 5.2 months of age, with clinical and laboratory diagnosis of HUS were studied. Platelets from samples obtained 15 days, one month and two months after the beginning of the disease were isolated. Normal platelets controls of a similar age population were also analyzed. Membrane glycoproteins (GP) were solubilized and studied by Western blot. Specific monoclonal antibodies were used. Low levels of GPIb and GPIIbIIIa were observed in the first sample. Then, GPIb increased quickly, reaching normal values on day 30. GPIIbIIIA complex increased slightly on day 60, but always showed low levels (5- 25%). GPIIb kept a low level throughout the study, while GPIIIa presented values near the lower normal level (25-50%) From these findings it can be concluded that protein synthesis is not altered since GPIb increases rapidly, GPIIbIIIa is the mainly damaged receptor. It can be due to a blockage by non-specific antibodies or RGD peptides or may be some structural alteration on GPIIb by non-specific proteolysis. |
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