Dye( arc)mutants: Insights into an unexplained phenotype and its suppression by the synthesis of poly (3-hydroxybutyrate) in Escherichia coli recombinants
arcA codes for a central regulator in Escherichia coli that responds to redox conditions of growth. Mutations in this gene, originally named dye, confer sensitivity to toluidine blue and other redox dyes. However, the molecular basis for the dye-sensitive phenotype has not been elucidated. In this w...
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todo:paper_03781097_v258_n1_p55_Ruiz2023-10-03T15:31:42Z Dye( arc)mutants: Insights into an unexplained phenotype and its suppression by the synthesis of poly (3-hydroxybutyrate) in Escherichia coli recombinants Ruiz, J.A. Fernández, R.O. Nikel, P.I. Méndez, B.S. Pettinari, M.J. arcA O2 consumption PHB ROS Toluidine blue poly(3 hydroxybutyric acid) tolonium chloride aerobic metabolism article controlled study Escherichia coli gene mutation genetic code molecular dynamics nonhuman oxidation reduction reaction oxygen consumption phenotype polymerization priority journal Bacterial Outer Membrane Proteins Escherichia coli Escherichia coli Proteins Hydroxybutyrates Mutation Oxygen Consumption Phenotype Polyesters Reactive Oxygen Species Recombination, Genetic Repressor Proteins Arca Escherichia coli arcA codes for a central regulator in Escherichia coli that responds to redox conditions of growth. Mutations in this gene, originally named dye, confer sensitivity to toluidine blue and other redox dyes. However, the molecular basis for the dye-sensitive phenotype has not been elucidated. In this work, we show that toluidine blue redirects electrons to O2 and causes an increase in the generation of reactive O2 species (ROS). We also demonstrate that synthesis of poly (3-hydroxybutyrate) suppresses the Dye phenotype in E. coli recombinants, as the capacity to synthesize the polymer reduces sensitivity to toluidine blue, O2 consumption and ROS production levels. © 2006 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved. Fil:Ruiz, J.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Méndez, B.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Pettinari, M.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_03781097_v258_n1_p55_Ruiz |
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Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
arcA O2 consumption PHB ROS Toluidine blue poly(3 hydroxybutyric acid) tolonium chloride aerobic metabolism article controlled study Escherichia coli gene mutation genetic code molecular dynamics nonhuman oxidation reduction reaction oxygen consumption phenotype polymerization priority journal Bacterial Outer Membrane Proteins Escherichia coli Escherichia coli Proteins Hydroxybutyrates Mutation Oxygen Consumption Phenotype Polyesters Reactive Oxygen Species Recombination, Genetic Repressor Proteins Arca Escherichia coli |
| spellingShingle |
arcA O2 consumption PHB ROS Toluidine blue poly(3 hydroxybutyric acid) tolonium chloride aerobic metabolism article controlled study Escherichia coli gene mutation genetic code molecular dynamics nonhuman oxidation reduction reaction oxygen consumption phenotype polymerization priority journal Bacterial Outer Membrane Proteins Escherichia coli Escherichia coli Proteins Hydroxybutyrates Mutation Oxygen Consumption Phenotype Polyesters Reactive Oxygen Species Recombination, Genetic Repressor Proteins Arca Escherichia coli Ruiz, J.A. Fernández, R.O. Nikel, P.I. Méndez, B.S. Pettinari, M.J. Dye( arc)mutants: Insights into an unexplained phenotype and its suppression by the synthesis of poly (3-hydroxybutyrate) in Escherichia coli recombinants |
| topic_facet |
arcA O2 consumption PHB ROS Toluidine blue poly(3 hydroxybutyric acid) tolonium chloride aerobic metabolism article controlled study Escherichia coli gene mutation genetic code molecular dynamics nonhuman oxidation reduction reaction oxygen consumption phenotype polymerization priority journal Bacterial Outer Membrane Proteins Escherichia coli Escherichia coli Proteins Hydroxybutyrates Mutation Oxygen Consumption Phenotype Polyesters Reactive Oxygen Species Recombination, Genetic Repressor Proteins Arca Escherichia coli |
| description |
arcA codes for a central regulator in Escherichia coli that responds to redox conditions of growth. Mutations in this gene, originally named dye, confer sensitivity to toluidine blue and other redox dyes. However, the molecular basis for the dye-sensitive phenotype has not been elucidated. In this work, we show that toluidine blue redirects electrons to O2 and causes an increase in the generation of reactive O2 species (ROS). We also demonstrate that synthesis of poly (3-hydroxybutyrate) suppresses the Dye phenotype in E. coli recombinants, as the capacity to synthesize the polymer reduces sensitivity to toluidine blue, O2 consumption and ROS production levels. © 2006 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved. |
| format |
JOUR |
| author |
Ruiz, J.A. Fernández, R.O. Nikel, P.I. Méndez, B.S. Pettinari, M.J. |
| author_facet |
Ruiz, J.A. Fernández, R.O. Nikel, P.I. Méndez, B.S. Pettinari, M.J. |
| author_sort |
Ruiz, J.A. |
| title |
Dye( arc)mutants: Insights into an unexplained phenotype and its suppression by the synthesis of poly (3-hydroxybutyrate) in Escherichia coli recombinants |
| title_short |
Dye( arc)mutants: Insights into an unexplained phenotype and its suppression by the synthesis of poly (3-hydroxybutyrate) in Escherichia coli recombinants |
| title_full |
Dye( arc)mutants: Insights into an unexplained phenotype and its suppression by the synthesis of poly (3-hydroxybutyrate) in Escherichia coli recombinants |
| title_fullStr |
Dye( arc)mutants: Insights into an unexplained phenotype and its suppression by the synthesis of poly (3-hydroxybutyrate) in Escherichia coli recombinants |
| title_full_unstemmed |
Dye( arc)mutants: Insights into an unexplained phenotype and its suppression by the synthesis of poly (3-hydroxybutyrate) in Escherichia coli recombinants |
| title_sort |
dye( arc)mutants: insights into an unexplained phenotype and its suppression by the synthesis of poly (3-hydroxybutyrate) in escherichia coli recombinants |
| url |
http://hdl.handle.net/20.500.12110/paper_03781097_v258_n1_p55_Ruiz |
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