The Alzheimer-related gene presenilin-1 facilitates sonic hedgehog expression in Xenopus primary neurogenesis

We analyzed the influence of presenilins on the genetic cascades that control neuronal differentiation in Xenopus embryos. Resembling sonic hedgehog (shh) overexpression, presenilin mRNA injection reduced the number of N-tubulin+ primary neurons and modulated Gli3 and Zic2 according to their roles i...

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Detalles Bibliográficos
Autores principales: Paganelli, A.R., Ocaña, O., Prat, M.I., Franco, P.G., López, S.L., Morelli, L., Adamo, A.M., Riccomagno, M.M., Matsubara, E., Shoji, M., Affranchino, J.L., Castaño, E.M., Carrasco, A.E.
Formato: JOUR
Materias:
Apoptosis
Morpholino oligonucleotide
Presenilin
Primary neurogenesis
Proliferation
Sonic hedgehog
Xenopus laevis
antisense oligonucleotide
messenger RNA
presenilin 1
sonic hedgehog protein
Alzheimer disease
apoptosis
article
controlled study
embryo
gene expression regulation
immunohistochemistry
nervous system development
nonhuman
priority journal
reverse transcription polymerase chain reaction
Xenopus
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_09254773_v107_n1-2_p119_Paganelli
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
Descripción
Sumario:We analyzed the influence of presenilins on the genetic cascades that control neuronal differentiation in Xenopus embryos. Resembling sonic hedgehog (shh) overexpression, presenilin mRNA injection reduced the number of N-tubulin+ primary neurons and modulated Gli3 and Zic2 according to their roles in activating and repressing primary neurogenesis, respectively. Presenilin increased shh expression within its normal domain, mainly in the floor plate, whereas an antisense X-presenilin-α morpholino oligonucleotide reduced shh expression. Both shh and presenilin promoted cell proliferation and apoptosis, but the effects of shh were widely distributed, while those resulting from presenilin injection coincided with the range of shh signaling. We suggest that presenilin may modulate primary neurogenesis, proliferation, and apoptosis in the neural plate, through the enhancement of shh signaling. Copyright © 2001 Elsevier Science Ireland Ltd.

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