Proposed molecular model for electrostatic interactions between insulin and chitosan. Nano-complexation and activity in cultured cells
The objective of this contribution was to propose a model that would explain the nanocomplexes formation between Human Recombinant Insulin (I) and a polydisperse Chitosan (CS). Such an objective implied exploring I and CS concentration conditions that allowed the formation of complexes with defined...
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| Autores principales: | , , , , , |
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| Formato: | JOUR |
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_09277757_v537_n_p425_Silva |
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| Sumario: | The objective of this contribution was to propose a model that would explain the nanocomplexes formation between Human Recombinant Insulin (I) and a polydisperse Chitosan (CS). Such an objective implied exploring I and CS concentration conditions that allowed the formation of complexes with defined and reproducible submicronic dimensions. I-CS complexes were obtained by mixing I and CS solutions at pH 2 and then increasing the pH up to 6 promoting electrostatic interactions between them. Colloidal stages of I and I-CS nano-complexes formation were characterized by dynamic light scattering (DLS), ζ-potential, solutions flow behavior and absorbance measurements. 1·10−2%, w/w, of CS allowed covering completely the surface protein aggregates constituting core–shell nano-structures of 200 nm, with a ζ-potential of 17,5 mV. Solution dynamic viscosity results kept relation with different stages of nano-complexation process. Biological activity of I-CS complexes was studied in 3T3-L1 cultured fibroblast showing a delayed and sustained activity as compared to free insulin. I-CS nano-complexes could be an alternative for developing a new generation of drugs allowing I protection from the hostile conditions of the body and increasing its absorption. These findings have basic and practical impacts as they could be exploited to exert the controlled release of I in therapeutic formulations by using the I-CS nano-complexes. © 2017 Elsevier B.V. |
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