Electronic tongue for simultaneous detection of endotoxins and other contaminants of microbiological origin

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Endotoxins, also referred to as pyrogens, are lipopolysaccharides (LPS) present in the outer membrane of Gram-negative bacteria, and represent one of the most dangerous microbiological contaminants in water for hemodialysis and intravenous infusion. A method is presented for the simultaneous detecti...

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Detalles Bibliográficos
Autores principales:	Heras, J.Y., Pallarola, D., Battaglini, F.
Formato:	JOUR
Materias:	Electronic tongue Endotoxin detection Impedance spectroscopy Principal component analysis Pro-inflammatory molecules Antimicrobial proteins Bacterial lysis Binding activities Data interpretation Electrode surfaces Endotoxin neutralizing Gram-negative bacteria Inflammatory species Instrumental methods Lipopolysaccharides Multi variate analysis Multivariate discriminant analysis Outer membrane Purified water Regulatory level Simultaneous detection Spectral data Amino acids Bacteria Bacteriology Cluster analysis Decision making Dialysis Discriminant analysis Drug products Electrochemical corrosion Electrochemical impedance spectroscopy Electrodes Electronic tongues Multivariant analysis Nucleic acids Organic acids Phospholipids Proteins Spectrum analysis amino acid cationic antimicrobial protein endotoxin endotoxin neutralizing protein lipopolysaccharide nucleic acid phospholipid protein unclassified drug biomimetic material drug mixture article bacterium contamination cluster analysis controlled study discriminant analysis electrochemical impedance spectroscopy electrode electronics nonhuman principal component analysis protein binding tongue toxin analysis analysis conductometry device failure analysis devices equipment design evaluation study genetic procedures metabolism reproducibility Salmonella enterica sensitivity and specificity Biomimetic Materials Biosensing Techniques Complex Mixtures Conductometry Endotoxins Equipment Design Equipment Failure Analysis Reproducibility of Results Sensitivity and Specificity Tongue Bacteria (microorganisms) Negibacteria
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_09565663_v25_n11_p2470_Heras
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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Sumario:	Endotoxins, also referred to as pyrogens, are lipopolysaccharides (LPS) present in the outer membrane of Gram-negative bacteria, and represent one of the most dangerous microbiological contaminants in water for hemodialysis and intravenous infusion. A method is presented for the simultaneous detection of endotoxins and other bacterial lysis contaminating species in purified water for parenteral formulations. The technique used is electrochemical impedance spectroscopy, with data interpretation using principal component analysis (PCA), cluster analysis (CA), and multivariate discriminant analysis (MDA). Two types of electrode surfaces were modified with LPS recognition agents: (i) a 37 amino acids fragment of a 18. kDa cationic antimicrobial protein (CAP18F) that has LPS binding activity; (ii) the highly selective endotoxin neutralizing protein (ENP). Statistical multivariate analysis of the impedance spectral data allowed the detection of endotoxin at, and below, the threshold pharmaceutical regulatory level. Discrimination of LPS from samples containing proteins, nucleic acids, phospholipids or their mixtures was achieved. These results open a new route to a practical instrumental method capable of detecting and discriminating LPS from other potential pro-inflammatory species of microbiological origin, such as nucleic acids. © 2010 Elsevier B.V.

Electronic tongue for simultaneous detection of endotoxins and other contaminants of microbiological origin

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