A method for the preparation of Tetrahymena thermophila phospholipase A1 suitable for large-scale production

A rapid and economical method for the purification of phospholipase A1 (PLA1) from the extracellular medium of the ciliate Tetrahymena thermophila is presented. Essentially, the procedure, here designated as purification by selective interaction (PSI), entails the incubation of media containing PLA1...

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Detalles Bibliográficos
Autores principales:	Guberman, A., Hartmann, M., Tiedtke, A., Florin-Christensen, J., Florin-Christensen, M.
Formato:	JOUR
Materias:	calcium chloride diethylaminoethyl cellulose liposome n,n dimethylformamide phospholipase a1 phospholipid article centrifugation enzyme activity enzyme purification ion exchange chromatography nonhuman polyacrylamide gel electrophoresis precipitation tetrahymena thermophila Animals Culture Media Liposomes Phospholipases A Tetrahymena thermophila Ciliophora Phaseolus (angiosperm) Protozoa
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_13645072_v86_n2_p226_Guberman
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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