Redox molecule based SERS sensors

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We describe a general framework to design nanobiosensors based on a wired enzyme coupled to a redox molecule and integrated with SERS Au core-shell nanoparticles and ordered nanocavities. The response of the proposed sensor is based on the different electronic resonant Raman behavior of the oxidized...

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Autores principales: Tognalli, N.G., Scodeller, P., Flexer, V., Szamocki, R., Ricci, A., Tagliazucchi, M., Calvo, E.J., Fainstein, A.
Formato: JOUR
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_14639076_v11_n34_p7412_Tognalli
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_14639076_v11_n34_p7412_Tognalli
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spelling todo:paper_14639076_v11_n34_p7412_Tognalli2023-10-03T16:16:43Z Redox molecule based SERS sensors Tognalli, N.G. Scodeller, P. Flexer, V. Szamocki, R. Ricci, A. Tagliazucchi, M. Calvo, E.J. Fainstein, A. We describe a general framework to design nanobiosensors based on a wired enzyme coupled to a redox molecule and integrated with SERS Au core-shell nanoparticles and ordered nanocavities. The response of the proposed sensor is based on the different electronic resonant Raman behavior of the oxidized or reduced electronic states of the molecular wire, and on the surface plasmon amplification induced by the tailored metallic substrate. The nanobiosensors can be interrogated remotely through the resonant Raman scattering intensity recovery or spectral variation of the redox molecule, an Os-complex, when the latter varies its oxidation state. Alternatively, we show through two-color spectro-electrochemistry that Raman scattering is also finely sensitive to oxidation state changes of flavin, a biomimetic system that mimics the active center of many flavoprotein enzymes. We show that multiple sample spectroscopic ellipsometry gives access to the spectral dependence of the optical constants of single redox-molecule layers, and through it to the electronic resonances of the system. All the components for selective molecular recognition and for the generation of an optical amplified signal, are self-contained in the proposed biosensor. As proof of concept a compact SERS sensor responsive to glucose with millimolar concentration in solution is demonstrated. © the Owner Societies. Fil:Scodeller, P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Flexer, V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Ricci, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Tagliazucchi, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Calvo, E.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_14639076_v11_n34_p7412_Tognalli
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
description We describe a general framework to design nanobiosensors based on a wired enzyme coupled to a redox molecule and integrated with SERS Au core-shell nanoparticles and ordered nanocavities. The response of the proposed sensor is based on the different electronic resonant Raman behavior of the oxidized or reduced electronic states of the molecular wire, and on the surface plasmon amplification induced by the tailored metallic substrate. The nanobiosensors can be interrogated remotely through the resonant Raman scattering intensity recovery or spectral variation of the redox molecule, an Os-complex, when the latter varies its oxidation state. Alternatively, we show through two-color spectro-electrochemistry that Raman scattering is also finely sensitive to oxidation state changes of flavin, a biomimetic system that mimics the active center of many flavoprotein enzymes. We show that multiple sample spectroscopic ellipsometry gives access to the spectral dependence of the optical constants of single redox-molecule layers, and through it to the electronic resonances of the system. All the components for selective molecular recognition and for the generation of an optical amplified signal, are self-contained in the proposed biosensor. As proof of concept a compact SERS sensor responsive to glucose with millimolar concentration in solution is demonstrated. © the Owner Societies.
format JOUR
author Tognalli, N.G.
Scodeller, P.
Flexer, V.
Szamocki, R.
Ricci, A.
Tagliazucchi, M.
Calvo, E.J.
Fainstein, A.
spellingShingle Tognalli, N.G.
Scodeller, P.
Flexer, V.
Szamocki, R.
Ricci, A.
Tagliazucchi, M.
Calvo, E.J.
Fainstein, A.
Redox molecule based SERS sensors
author_facet Tognalli, N.G.
Scodeller, P.
Flexer, V.
Szamocki, R.
Ricci, A.
Tagliazucchi, M.
Calvo, E.J.
Fainstein, A.
author_sort Tognalli, N.G.
title Redox molecule based SERS sensors
title_short Redox molecule based SERS sensors
title_full Redox molecule based SERS sensors
title_fullStr Redox molecule based SERS sensors
title_full_unstemmed Redox molecule based SERS sensors
title_sort redox molecule based sers sensors
url http://hdl.handle.net/20.500.12110/paper_14639076_v11_n34_p7412_Tognalli
work_keys_str_mv AT tognalling redoxmoleculebasedserssensors
AT scodellerp redoxmoleculebasedserssensors
AT flexerv redoxmoleculebasedserssensors
AT szamockir redoxmoleculebasedserssensors
AT riccia redoxmoleculebasedserssensors
AT tagliazucchim redoxmoleculebasedserssensors
AT calvoej redoxmoleculebasedserssensors
AT fainsteina redoxmoleculebasedserssensors
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