Nitroxide amide-BODIPY probe behavior in fibroblasts analyzed by advanced fluorescence microscopy

A novel synthesized nitroxide amide-BODIPY prefluorescent probe was used to study cellular redox balance that modulates nitroxide/hydroxylamine ratio in cultured human fibroblasts. FLIM quantitatively differentiated between nitroxide states of the cytoplasm-localized probe imaged by TIRF, monitoring...

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Detalles Bibliográficos
Autores principales:	Liras, M., Simoncelli, S., Rivas-Aravena, A., García, O., Scaiano, J.C., Alarcon, E.I., Aspée, A.
Formato:	JOUR
Materias:	Amides Cell culture Cytology Fibroblasts Fluorescence Fluorescence microscopy Cellular redox Fluorescence intensities Human fibroblast Nitroxides Probes 4,4-difluoro-4-bora-3a,4a-diaza-s-indacene amide boron derivative fluorescent dye hydrogen peroxide nitrogen oxide cell culture chemistry fibroblast fluorescence microscopy human metabolism oxidation reduction reaction synthesis Boron Compounds Cells, Cultured Fluorescent Dyes Humans Hydrogen Peroxide Microscopy, Fluorescence Nitrogen Oxides Oxidation-Reduction
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_14770520_v14_n17_p4023_Liras
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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