Teratogenic Evaluation of Metronidazole and Ornidazole Using Drosophila melanogaster as an Experimental Model

BACKGROUND: Drosophila and vertebrates show similarities that suggest that the mechanisms involved in the induction of developmental defects may be similar in both. Therefore, Drosophila has been proposed as a useful, rapid, and economical model in the preliminary screening for teratology studies. T...

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Detalles Bibliográficos
Autores principales: Palermo, A.M., Reynoso, A.S., López Nigro, M., Carballo, M.A., Mudry, M.D.
Formato: JOUR
Materias:
metronidazole
nitroimidazole derivative
ornidazole
animal cell
animal experiment
animal model
animal tissue
article
bioassay
concentration response
controlled study
developing country
developmental biology
developmental disorder
Drosophila melanogaster
evaluation
experimental model
female
incidence
larval development
morphological trait
newborn
nonhuman
priority journal
regulatory mechanism
screening test
sensitivity analysis
sensitivity and specificity
teratogenicity
toxicity testing
vertebrate
wild type
Abdomen
Abnormalities, Drug-Induced
Animals
Antiprotozoal Agents
Larva
Metronidazole
Ornidazole
Thorax
Melanogaster
Vertebrata
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_15420752_v70_n4_p157_Palermo
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
Descripción
Sumario:BACKGROUND: Drosophila and vertebrates show similarities that suggest that the mechanisms involved in the induction of developmental defects may be similar in both. Therefore, Drosophila has been proposed as a useful, rapid, and economical model in the preliminary screening for teratology studies. The objective of the present study was to investigate the effect of metronidazole (MTZ) and omidazole (ONZ) on the developmental stages of Drosophila melanogaster. METHODS: Samarkand wild-type females were allowed to lay eggs for 24 hr in media containing MTZ or ONZ at concentrations of 0, 500, 1000, and 2000 μg/ml. When larvae completed their development, the emerging flies were counted and examined for morphological abnormalities. RESULTS: After the analysis of 400-1000 flies for each concentration, ONZ-treated flies did not show an incidence of malformations above control values, although a significant high number of individuals with reduced body size was observed (p < 0.005, χ2 test). On the other hand, the 1000- and 2000-μg/ml MTZ-treated series presented higher frequencies of total abnormalities than did concurrent and historic controls (p < 0.05, χ2 test), indicating an MTZ effect during developmental morphogenesis. CONCLUSIONS: These findings contribute to the characterization of both nitroimidazoles, which are widely used, especially in underdeveloped countries. At the same time, this Drosophila bioassay is sensitive enough to detect differential effects of MTZ and ONZ (abnormalities vs. growth effects), showing specificity and selectivity. © 2004 Wiley-Liss, Inc.

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