The Dengue Virus NS5 Protein Intrudes in the Cellular Spliceosome and Modulates Splicing

Dengue virus NS5 protein plays multiple functions in the cytoplasm of infected cells, enabling viral RNA replication and counteracting host antiviral responses. Here, we demonstrate a novel function of NS5 in the nucleus where it interferes with cellular splicing. Using global proteomic analysis of...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	De Maio, F.A., Risso, G., Iglesias, N.G., Shah, P., Pozzi, B., Gebhard, L.G., Mammi, P., Mancini, E., Yanovsky, M.J., Andino, R., Krogan, N., Srebrow, A., Gamarnik, A.V.
Formato:	JOUR
Materias:	complementary DNA cystic fibrosis transmembrane conductance regulator monoclonal antibody nonstructural protein 5 polyclonal antibody protein bcl x small nuclear ribonucleoprotein STAT2 protein NS5 protein, dengue virus viral protein A549 cell line alternative RNA splicing Article cell fractionation controlled study dengue Dengue virus gene expression regulation genetic transfection hepatocellular carcinoma cell line high throughput sequencing host pathogen interaction human human cell immunofluorescence test protein localization protein protein interaction protein purification proteomics real time polymerase chain reaction RNA processing RNAi therapeutics spliceosome virus recombinant virus replication Western blotting animal cell line fluorescent antibody technique genetics host parasite interaction metabolism pathogenicity physiology polymerase chain reaction RNA splicing virology Animals Cell Line Dengue Dengue Virus Fluorescent Antibody Technique High-Throughput Nucleotide Sequencing Host-Parasite Interactions Humans Polymerase Chain Reaction Ribonucleoprotein, U5 Small Nuclear RNA Splicing Spliceosomes Transfection Viral Nonstructural Proteins
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_15537366_v12_n8_p_DeMaio
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

Ejemplares similares

The Dengue Virus NS5 Protein Intrudes in the Cellular Spliceosome and Modulates Splicing
por: Risso, Guillermo Javier, et al.
Publicado: (2016)

The heterogeneous nuclear ribonucleoprotein K (hnRNP K) is a host factor required for dengue virus and Junín virus multiplication
por: Scolaro, Luis Alberto, et al.
Publicado: (2015)

The heterogeneous nuclear ribonucleoprotein K (hnRNP K) is a host factor required for dengue virus and Junín virus multiplication
por: Brunetti, J.E., et al.

SUMO conjugation to spliceosomal proteins is required for efficient pre-mRNA splicing
Publicado: (2017)

SUMO conjugation to spliceosomal proteins is required for efficient pre-mRNA splicing
por: Pozzi, B., et al.

When SUMO met splicing
Publicado: (2018)

When SUMO met splicing
por: Pozzi, B., et al.

Differential effect of acute and persistent Junín virus infections on the nucleo-cytoplasmic trafficking and expression of heterogeneous nuclear ribonucleoproteins type A and B
por: Maeto, Cynthia A., et al.
Publicado: (2011)

Differential effect of acute and persistent Junín virus infections on the nucleo-cytoplasmic trafficking and expression of heterogeneous nuclear ribonucleoproteins type A and B
por: Maeto, C.A., et al.
Publicado: (2011)

Differential effect of acute and persistent Junín virus infections on the nucleo-cytoplasmic trafficking and expression of heterogeneous nuclear ribonucleoproteins type A and B
por: Maeto, C.A., et al.

Differential effect of acute and persistent Junín virus infections on the nucleo-cytoplasmic trafficking and expression of heterogeneous nuclear ribonucleoproteins type A and B
por: Maeto, C.A., et al.
Publicado: (2011)

Novel Staufen1 ribonucleoproteins prevent formation of stress granules but favour encapsidation of HIV-1 genomic RNA
por: Boccaccio, Graciela Lidia
Publicado: (2010)

Novel Staufen1 ribonucleoproteins prevent formation of stress granules but favour encapsidation of HIV-1 genomic RNA
por: Abrahamyan, L.G., et al.
Publicado: (2010)

Novel Staufen1 ribonucleoproteins prevent formation of stress granules but favour encapsidation of HIV-1 genomic RNA
por: Abrahamyan, L.G., et al.

Novel Staufen1 ribonucleoproteins prevent formation of stress granules but favour encapsidation of HIV-1 genomic RNA
por: Abrahamyan, L.G., et al.
Publicado: (2010)

Antiviral activity of an N-allyl acridone against dengue virus
por: Talarico, Laura Beatriz, et al.
Publicado: (2015)

Antiviral activity of an N-allyl acridone against dengue virus
por: Mazzucco, M.B., et al.

Requirement of cholesterol in the viral envelope for dengue virus infection
por: Damonte, Elsa Beatriz
Publicado: (2013)

Requirement of cholesterol in the viral envelope for dengue virus infection
por: Carro, A.C., et al.

Involvement of heterogeneous nuclear ribonucleoproteins in viral multiplication
por: Castilla, Viviana, et al.
Publicado: (2012)

Involvement of heterogeneous nuclear ribonucleoproteins in viral multiplication
por: Castilla, V., et al.

Characterization of in vitro dengue virus resistance to carrageenan
Publicado: (2016)

Characterization of in vitro dengue virus resistance to carrageenan
por: Talarico, L.B., et al.

Host cell factors as antiviral targets in arenavirus infection
por: Linero, Florencia N., et al.
Publicado: (2012)

Host cell factors as antiviral targets in arenavirus infection
por: Linero, F.N., et al.

A 5′ RNA element promotes dengue virus RNA synthesis on a circular genome
Publicado: (2006)

A 5′ RNA element promotes dengue virus RNA synthesis on a circular genome
por: Filomatori, C.V., et al.
Publicado: (2006)

A 5′ RNA element promotes dengue virus RNA synthesis on a circular genome
por: Filomatori, C.V., et al.

A 5′ RNA element promotes dengue virus RNA synthesis on a circular genome
por: Filomatori, C.V., et al.
Publicado: (2006)

How Are Short Exons Flanked by Long Introns Defined and Committed to Splicing?
por: Kornblihtt, Alberto Rodolfo
Publicado: (2016)

How Are Short Exons Flanked by Long Introns Defined and Committed to Splicing?
por: Hollander, D., et al.

The carboxy terminal domain of RNA polymerase II and alternative splicing
por: Muñoz, Manuel Javier, et al.
Publicado: (2010)

The carboxy terminal domain of RNA polymerase II and alternative splicing
por: Muñoz, M.J., et al.

Role of the ERK1/2 signaling pathway in the replication of junín and tacaribe viruses
Publicado: (2018)

Role of the ERK1/2 signaling pathway in the replication of junín and tacaribe viruses
por: Brunetti, J.E., et al.

Blockade of dengue virus entry into myeloid cells by endocytic inhibitors in the presence or absence of antibodies
Publicado: (2018)

Blockade of dengue virus entry into myeloid cells by endocytic inhibitors in the presence or absence of antibodies
por: Carro, A.C., et al.

Differential Requirements in Endocytic Trafficking for Penetration of Dengue Virus
por: Acosta, Eliana Gisela, et al.
Publicado: (2012)

Differential Requirements in Endocytic Trafficking for Penetration of Dengue Virus
por: Acosta, E.G., et al.
Publicado: (2012)

Differential Requirements in Endocytic Trafficking for Penetration of Dengue Virus
por: Acosta, E.G., et al.