Engineering a bifunctional copper site in the cupredoxin fold by loop-directed mutagenesis

Copper sites in proteins are designed to perform either electron transfer or redox catalysis. Type 1 and CuA sites are electron transfer hubs bound to a rigid protein fold that prevents binding of exogenous ligands and side reactions. Here we report the engineering of two Type 1 sites by loop-direct...

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Detalles Bibliográficos
Autores principales:	Espinoza-Cara, A., Zitare, U., Alvarez-Paggi, D., Klinke, S., Otero, L.H., Murgida, D.H., Vila, A.J.
Formato:	JOUR
Materias:	Binding sites Electron transitions Electronic structure Hydrogen bonds Ligands Mutagenesis Proteins Scaffolds (biology) Directed mutagenesis Electron transfer Exogenous ligands Functional features Hydrogen bond networks Natural proteins Reduction potential Strong interaction Copper
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_20416520_v9_n32_p6692_EspinozaCara
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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