Revisiting the role of interleukin-8 in chronic lymphocytic leukemia
The proliferation and survival of malignant B cells in chronic lymphocytic leukemia (CLL) depend on signals from the microenvironment in lymphoid tissues. Among a plethora of soluble factors, IL-8 has been considered one of the most relevant to support CLL B cell progression in an autocrine fashion,...
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todo:paper_20452322_v7_n1_p_Risnik2023-10-03T16:38:20Z Revisiting the role of interleukin-8 in chronic lymphocytic leukemia Risnik, D. Podaza, E. Almejún, M.B. Colado, A. Elías, E.E. Bezares, R.F. Fernández-Grecco, H. Cranco, S. Sánchez-Ávalos, J.C. Borge, M. Gamberale, R. Giordano, M. The proliferation and survival of malignant B cells in chronic lymphocytic leukemia (CLL) depend on signals from the microenvironment in lymphoid tissues. Among a plethora of soluble factors, IL-8 has been considered one of the most relevant to support CLL B cell progression in an autocrine fashion, even though the expression of IL-8 receptors, CXCR1 and CXCR2, on leukemic B cells has not been reported. Here we show that circulating CLL B cells neither express CXCR1 or CXCR2 nor they respond to exogenous IL-8 when cultured in vitro alone or in the presence of monocytes/nurse-like cells. By intracellular staining and ELISA we show that highly purified CLL B cells do not produce IL-8 spontaneously or upon activation through the B cell receptor. By contrast, we found that a minor proportion (<0.5%) of contaminating monocytes in enriched suspensions of leukemic cells might be the actual source of IL-8 due to their strong capacity to release this cytokine. Altogether our results indicate that CLL B cells are not able to secrete or respond to IL-8 and highlight the importance of methodological details in in vitro experiments. © 2017 The Author(s). JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_20452322_v7_n1_p_Risnik |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| description |
The proliferation and survival of malignant B cells in chronic lymphocytic leukemia (CLL) depend on signals from the microenvironment in lymphoid tissues. Among a plethora of soluble factors, IL-8 has been considered one of the most relevant to support CLL B cell progression in an autocrine fashion, even though the expression of IL-8 receptors, CXCR1 and CXCR2, on leukemic B cells has not been reported. Here we show that circulating CLL B cells neither express CXCR1 or CXCR2 nor they respond to exogenous IL-8 when cultured in vitro alone or in the presence of monocytes/nurse-like cells. By intracellular staining and ELISA we show that highly purified CLL B cells do not produce IL-8 spontaneously or upon activation through the B cell receptor. By contrast, we found that a minor proportion (<0.5%) of contaminating monocytes in enriched suspensions of leukemic cells might be the actual source of IL-8 due to their strong capacity to release this cytokine. Altogether our results indicate that CLL B cells are not able to secrete or respond to IL-8 and highlight the importance of methodological details in in vitro experiments. © 2017 The Author(s). |
| format |
JOUR |
| author |
Risnik, D. Podaza, E. Almejún, M.B. Colado, A. Elías, E.E. Bezares, R.F. Fernández-Grecco, H. Cranco, S. Sánchez-Ávalos, J.C. Borge, M. Gamberale, R. Giordano, M. |
| spellingShingle |
Risnik, D. Podaza, E. Almejún, M.B. Colado, A. Elías, E.E. Bezares, R.F. Fernández-Grecco, H. Cranco, S. Sánchez-Ávalos, J.C. Borge, M. Gamberale, R. Giordano, M. Revisiting the role of interleukin-8 in chronic lymphocytic leukemia |
| author_facet |
Risnik, D. Podaza, E. Almejún, M.B. Colado, A. Elías, E.E. Bezares, R.F. Fernández-Grecco, H. Cranco, S. Sánchez-Ávalos, J.C. Borge, M. Gamberale, R. Giordano, M. |
| author_sort |
Risnik, D. |
| title |
Revisiting the role of interleukin-8 in chronic lymphocytic leukemia |
| title_short |
Revisiting the role of interleukin-8 in chronic lymphocytic leukemia |
| title_full |
Revisiting the role of interleukin-8 in chronic lymphocytic leukemia |
| title_fullStr |
Revisiting the role of interleukin-8 in chronic lymphocytic leukemia |
| title_full_unstemmed |
Revisiting the role of interleukin-8 in chronic lymphocytic leukemia |
| title_sort |
revisiting the role of interleukin-8 in chronic lymphocytic leukemia |
| url |
http://hdl.handle.net/20.500.12110/paper_20452322_v7_n1_p_Risnik |
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