Imaging of calcium dynamics in pollen tube cytoplasm

Cytoplasmic calcium [(Ca2+)cyt] is a central component of cellular signal transduction pathways. In plants, many external and internal stimuli transiently elevate (Ca2+)cyt, initiating downstream responses that control different features of plant development. In pollen tubes the establishment of an...

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Autores principales: Barberini, M.L., Muschietti, J.
Formato: CHAP
Materias:
Calcium
Kymograph
Pollen
Ratio imaging
Yellow cameleon 3.6
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_97814939_v_n_p49_Barberini
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_97814939_v_n_p49_Barberini
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spelling todo:paper_97814939_v_n_p49_Barberini2023-10-03T16:43:45Z Imaging of calcium dynamics in pollen tube cytoplasm Barberini, M.L. Muschietti, J. Calcium Kymograph Pollen Ratio imaging Yellow cameleon 3.6 Cytoplasmic calcium [(Ca2+)cyt] is a central component of cellular signal transduction pathways. In plants, many external and internal stimuli transiently elevate (Ca2+)cyt, initiating downstream responses that control different features of plant development. In pollen tubes the establishment of an oscillatory gradient of calcium at the tip is essential for polarized growth. Disruption of the cytosolic Ca2+ gradient by chelators or channel blockers inhibits pollen tube growth. To quantify the physiological role of (Ca2+)cyt in cellular systems, genetically encoded Ca2+ indicators such as Yellow Cameleons (YCs) have been developed. The Cameleons are based on a fluorescence resonance energy transfer (FRET) process. Here, we describe a method for imaging cytoplasmic Ca2+ dynamics in growing pollen tubes that express the fluorescent calcium indicator Yellow Cameleon 3.6 (YC 3.6), using laser-scanning confocal microscopy. © Springer Science+Business Media New York 2015. All rights are reserved. Fil:Muschietti, J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. CHAP info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_97814939_v_n_p49_Barberini
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Calcium
Kymograph
Pollen
Ratio imaging
Yellow cameleon 3.6
spellingShingle Calcium
Kymograph
Pollen
Ratio imaging
Yellow cameleon 3.6
Barberini, M.L.
Muschietti, J.
Imaging of calcium dynamics in pollen tube cytoplasm
topic_facet Calcium
Kymograph
Pollen
Ratio imaging
Yellow cameleon 3.6
description Cytoplasmic calcium [(Ca2+)cyt] is a central component of cellular signal transduction pathways. In plants, many external and internal stimuli transiently elevate (Ca2+)cyt, initiating downstream responses that control different features of plant development. In pollen tubes the establishment of an oscillatory gradient of calcium at the tip is essential for polarized growth. Disruption of the cytosolic Ca2+ gradient by chelators or channel blockers inhibits pollen tube growth. To quantify the physiological role of (Ca2+)cyt in cellular systems, genetically encoded Ca2+ indicators such as Yellow Cameleons (YCs) have been developed. The Cameleons are based on a fluorescence resonance energy transfer (FRET) process. Here, we describe a method for imaging cytoplasmic Ca2+ dynamics in growing pollen tubes that express the fluorescent calcium indicator Yellow Cameleon 3.6 (YC 3.6), using laser-scanning confocal microscopy. © Springer Science+Business Media New York 2015. All rights are reserved.
format CHAP
author Barberini, M.L.
Muschietti, J.
author_facet Barberini, M.L.
Muschietti, J.
author_sort Barberini, M.L.
title Imaging of calcium dynamics in pollen tube cytoplasm
title_short Imaging of calcium dynamics in pollen tube cytoplasm
title_full Imaging of calcium dynamics in pollen tube cytoplasm
title_fullStr Imaging of calcium dynamics in pollen tube cytoplasm
title_full_unstemmed Imaging of calcium dynamics in pollen tube cytoplasm
title_sort imaging of calcium dynamics in pollen tube cytoplasm
url http://hdl.handle.net/20.500.12110/paper_97814939_v_n_p49_Barberini
work_keys_str_mv AT barberiniml imagingofcalciumdynamicsinpollentubecytoplasm
AT muschiettij imagingofcalciumdynamicsinpollentubecytoplasm
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