Effect of embryo aggregation on in vitro development of adipose-derived mesenchymal stem cell - derived bovine clones
Somatic cell nuclear transfer (SCNT) is a method with unique ability to reprogram the epigenome of a fully differentiated cell. However, its efficiency remains extremely low. In this work, we assessed and combined two simple strategies to improve the SCNT efficiency in the bovine. These are the use...
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| Otros Autores: | , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
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| Acceso en línea: | http://ri.agro.uba.ar/files/intranet/articulo/2021savy.pdf LINK AL EDITOR |
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| 022 | |a 2152-4971 | ||
| 024 | |a 10.1089/cell.2021.0026 | ||
| 040 | |a AR-BaUFA |c AR-BaUFA | ||
| 245 | 0 | 0 | |a Effect of embryo aggregation on in vitro development of adipose-derived mesenchymal stem cell - derived bovine clones |
| 520 | |a Somatic cell nuclear transfer (SCNT) is a method with unique ability to reprogram the epigenome of a fully differentiated cell. However, its efficiency remains extremely low. In this work, we assessed and combined two simple strategies to improve the SCNT efficiency in the bovine. These are the use of less-differentiated donor cells to facilitate nuclear reprogramming and the embryo aggregation [EA] strategy that is thought to compensate for aberrant epigenome reprogramming. We carefully assessed the optimal time of EA by using in vitrofertilized (IVF) embryos and evaluated whether the use of adipose-derived mesenchymal stem cells [ASCs] as donor for SCNT together with EA improves the blastocyst rates and quality. Based on our results, we determined that the EA improves the preimplantation embryo development per well of IVF and SCNT embryos. We also demonstrated that day 0 (D0) is the optimal aggregation time that leads to a single blastocyst with uniform distribution of the original blastomeres. This was confirmed in bovine IVF embryos and then, the optimal condition was translated to SCNT embryos. Notably, the relative expression of the trophectoderm (TE) marker KRT18 was significantly different between aggregated and nonaggregated ASC-derived embryos. In the bovine, no effect of the donor cell is observed on the developmental rate, or the embryo quality. Therefore, no synergistic effect of the use of both strategies is observed. Our results suggest that EA at D0 is a simple and accessible strategy that improves the blastocyst rate per well in bovine SCNT and IVF embryos and influence the expression of a TE-related marker. The aggregation of two ASC-derived embryos seems to positively affect the embryo quality, which may improve the postimplantation development. | ||
| 650 | |2 Agrovoc |9 26 | ||
| 653 | |a MESENCHYMAL CELLS | ||
| 653 | |a AGGREGATION | ||
| 653 | |a SCNT | ||
| 653 | |a BOVINE | ||
| 700 | 1 | |a Savy, Virginia |u Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Producción Animal. Cátedra de Fisiología Animal. Laboratorio de Biotecnología Animal (LabBA). Buenos Aires, Argentina. |u Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigaciones en Producción Animal (INPA). Buenos Aires, Argentina. |u CONICET - Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigaciones en Producción Animal (INPA). Buenos Aires, Argentina. |9 68562 | |
| 700 | 1 | |a Alberio, Virgilia |u Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Producción Animal. Cátedra de Fisiología Animal. Laboratorio de Biotecnología Animal (LabBA). Buenos Aires, Argentina. |u Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigaciones en Producción Animal (INPA). Buenos Aires, Argentina. |u CONICET - Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigaciones en Producción Animal (INPA). Buenos Aires, Argentina. |9 69708 | |
| 700 | 1 | |a Vans Landschoot, Geraldina |u Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Producción Animal. Cátedra de Fisiología Animal. Laboratorio de Biotecnología Animal (LabBA). Buenos Aires, Argentina. |u Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigaciones en Producción Animal (INPA). Buenos Aires, Argentina. |u CONICET - Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigaciones en Producción Animal (INPA). Buenos Aires, Argentina. |9 74845 | |
| 700 | 1 | |a Moro, Lucía Natalia |u Fundación para la Lucha contra las Enfermedades Neurológicas de la Infancia (FLENI). Laboratorio de Investigación Aplicada a Neurociencias (LIAN). Buenos Aires, Argentina. |u CONICET - Fundación para la Lucha contra las Enfermedades Neurológicas de la Infancia (FLENI). Laboratorio de Investigación Aplicada a Neurociencias (LIAN). Buenos Aires, Argentina. |9 74846 | |
| 700 | 1 | |a Olea, Fernanda Daniela |u Universidad Favaloro. Instituto de Medicina Traslacional, Trasplante y Bioingeniería (IMETTYB), Buenos Aires, Argentina. |u CONICET - Universidad Favaloro. Instituto de Medicina Traslacional, Trasplante y Bioingeniería (IMETTYB), Buenos Aires, Argentina. |9 74847 | |
| 700 | 1 | |a Rodriguez Alvarez, Lleretny |u Universidad de Concepción. Facultad de Ciencias Veterinarias. Departamento de Ciencia Animal. Concepción, Chile. |9 71846 | |
| 700 | 1 | |a Salamone, Daniel Felipe |u Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Producción Animal. Cátedra de Fisiología Animal. Laboratorio de Biotecnología Animal (LabBA). Buenos Aires, Argentina. |u Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigaciones en Producción Animal (INPA). Buenos Aires, Argentina. |u CONICET - Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigaciones en Producción Animal (INPA). Buenos Aires, Argentina. |9 61021 | |
| 773 | |g Vol.23, no.5 (2021), p.277-289, tbls., grafs. |t Cellular Reprogramming | ||
| 856 | |f 2021savy |i en reservorio |q application/pdf |u http://ri.agro.uba.ar/files/intranet/articulo/2021savy.pdf |x ARTI202306 | ||
| 856 | |u http://www.liebertpub.com/default.aspx |z LINK AL EDITOR | ||
| 942 | |c ARTICULO | ||
| 942 | |c ENLINEA | ||
| 976 | |a AAG | ||