Transcriptional response of peripheral blood mononuclear cells from cattle infected with Mycobacterium bovis

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Mycobacterium bovis is the causative agent of most cases of bovine tuberculosis. The identification of bTB biomarkers in specific stages of the disease will contribute to a better understanding of the immunopathology associated with tuberculosis and will enable their use in disease diagnosis and pro...

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Detalles Bibliográficos
Otros Autores: Blanco, Federico Carlos, Soria, M. A., Bianco, María Verónica, Bigi, Fabiana
Formato: Artículo
Lenguaje:Inglés
Materias:
AMPHIREGULIN B
BIOLOGICAL MARKER
CALGRANULIN B
CD14 ANTIGEN
CHEMOKINE RECEPTOR CXCR2
CXCL9 CHEMOKINE
FC FRAGMENT IMMUNOGLOBULIN A RECEPTOR
GELATINASE B
HYALURONAN SYNTHASE 2
INTERLEUKIN 1 RECEPTOR
INTERLEUKIN 13 RECEPTOR ALPHA1
INTERLEUKIN 22
LOW DENSITY LIPOPROTEIN RECEPTOR RELATED PROTEIN
MANNOSE RECEPTOR C TYPE 1
MATRIX METALLOPROTEINASE 19
MONOCYTE CHEMOTACTIC PROTEIN 2
NEUROPILIN 1
RESISTIN
RNA
SH3 DOMAIN BINDING PROTEIN 4
THROMBOCYTE FACTOR 4
TRANSCRIPTION FACTOR
TRANSCRIPTION FACTOR FYVE
TRANSCRIPTION FACTOR GZMA
TRANSCRIPTION FACTOR THBS1
TRANSFORMING GROWTH FACTOR BETA
TRANSGLUTAMINASE 3
UNCLASSIFIED DRUG
UROKINASE RECEPTOR
VERY LOW DENSITY LIPOPROTEIN RECEPTOR
ADAM8 GENE
ADAMTSL4 GENE
ANIMAL CELL
ANIMAL EXPERIMENT
ANIMAL MODEL
ANIMAL TISSUE
APOPTOSIS
AREGB GENE
ARHGAP21 GENE
BOVINE TUBERCULOSIS
CATTLE DISEASE
CCL2 GENE
CCL8 GENE
CCR1 GENE
CD14 GENE
CELL TRANSPORT
CSF3R GENE
CXCL10 GENE
CXCL9 GENE
CXCR2 GENE
DEFBIO GENE
DOWN REGULATION
ECM1 GENE
ENDOCYTOSIS
FCAR GENE
FCGR1A GENE
FYVE GENE
GENE
GENE EXPRESSION PROFILING
GENETIC VARIABILITY
GZMA GENE
HAS2 GENE
IL 18 GENE
IL 1R GENE
IL 6 GENE
IL13RA1 GENE
IL1R1 GENE
IL1RN GENE
IL22 GENE
IMMUNE RESPONSE
INFLAMMATION
LOC508666 GENE
LOC533894 GENE
MAPK13 GENE
MAPK14 GENE
MICROARRAY ANALYSIS
MMP19 GENE
MMP3 GENE
MMP9 GENE
MRC1 GENE
MYCOBACTERIUM BOVIS
MYD88 GENE
NFAM1 GENE
NONHUMAN
NRP1 GENE
PERIPHERAL BLOOD MONONUCLEAR CELL
PF4 GENE
PLAUR GENE
PROTEIN DEGRADATION
PYCARD GENE
QUANTITATIVE ANALYSIS
RETN GENE
REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION
S100A9 GENE
SASH1 GENE
SH3BP4 GENE
TGFBI GENE
TGM3 GENE
THBS1 GENE
TREM1 GENE
TYROBP GENE
UPREGULATION
VLDLR GENE
ANIMALS
BIOLOGICAL MARKERS
CATTLE
GENE EXPRESSION REGULATION, BACTERIAL
LEUKOCYTES, MONONUCLEAR
MODELS, GENETIC
OLIGONUCLEOTIDE ARRAY SEQUENCE ANALYSIS
TRANSCRIPTION, GENETIC
TUBERCULOSIS, BOVINE
BOS
BOVINAE
Acceso en línea:http://ri.agro.uba.ar/files/download/articulo/2012Blanco.pdf
LINK AL EDITOR.
Aporte de:Registro referencial: Solicitar el recurso aquí
Biblioteca Central (FAUBA) de Universidad de Buenos Aires
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022 |a 1932-6203 
024 |a 10.1371/journal.pone.0041066 
040 |a AR-BaUFA  |c AR-BaUFA 
245 1 0 |a Transcriptional response of peripheral blood mononuclear cells from cattle infected with Mycobacterium bovis 
520 |a Mycobacterium bovis is the causative agent of most cases of bovine tuberculosis. The identification of bTB biomarkers in specific stages of the disease will contribute to a better understanding of the immunopathology associated with tuberculosis and will enable their use in disease diagnosis and prognosis. The aim of this study was to evaluate the gene expression profile induced after specific stimulation of bovine peripheral blood mononuclear cells from cattle infected with M. bovis using the Affymetrix® GeneChip® Bovine Genome Array. A total of 172 genes showed differential expression profile that was statistically significant with log2-fold change grather than 2.5 and less than -2.5. Twenty-four out of these genes were upregulated and 148 were downregulated in bovine peripheral blood mononuclear cells of M. bovis-infected cattle. The highest differentially-expressed genes were related to immune and inflammatory responses, apoptosis, endocytosis, cellular trafficking and genes encoding proteins involved in cellular matrix degradation. Microarray results were confirmed in another group of infected cattle by RT-qPCR for the CD14, IL-1R, THBS1, MMP9 and FYVE genes. This study confirms previous findings that have shown that M. bovis infection in cattle results in the downregulation of immune response-related genes. Moreover, it validates the use of microarray platforms in combination with RT-qPCR to identify biomarkers of bovine tuberculosis. In addition, we propose CD14, IL-1R, THBS1, MMP9 and FYVE as potential biomarkers of bovine tuberculosis. 
653 0 |a AMPHIREGULIN B 
653 0 |a BIOLOGICAL MARKER 
653 0 |a CALGRANULIN B 
653 0 |a CD14 ANTIGEN 
653 0 |a CHEMOKINE RECEPTOR CXCR2 
653 0 |a CXCL9 CHEMOKINE 
653 0 |a FC FRAGMENT IMMUNOGLOBULIN A RECEPTOR 
653 0 |a GELATINASE B 
653 0 |a HYALURONAN SYNTHASE 2 
653 0 |a INTERLEUKIN 1 RECEPTOR 
653 0 |a INTERLEUKIN 13 RECEPTOR ALPHA1 
653 0 |a INTERLEUKIN 22 
653 0 |a LOW DENSITY LIPOPROTEIN RECEPTOR RELATED PROTEIN 
653 0 |a MANNOSE RECEPTOR C TYPE 1 
653 0 |a MATRIX METALLOPROTEINASE 19 
653 0 |a MONOCYTE CHEMOTACTIC PROTEIN 2 
653 0 |a NEUROPILIN 1 
653 0 |a RESISTIN 
653 0 |a RNA 
653 0 |a SH3 DOMAIN BINDING PROTEIN 4 
653 0 |a THROMBOCYTE FACTOR 4 
653 0 |a TRANSCRIPTION FACTOR 
653 0 |a TRANSCRIPTION FACTOR FYVE 
653 0 |a TRANSCRIPTION FACTOR GZMA 
653 0 |a TRANSCRIPTION FACTOR THBS1 
653 0 |a TRANSFORMING GROWTH FACTOR BETA 
653 0 |a TRANSGLUTAMINASE 3 
653 0 |a UNCLASSIFIED DRUG 
653 0 |a UROKINASE RECEPTOR 
653 0 |a VERY LOW DENSITY LIPOPROTEIN RECEPTOR 
653 0 |a ADAM8 GENE 
653 0 |a ADAMTSL4 GENE 
653 0 |a ANIMAL CELL 
653 0 |a ANIMAL EXPERIMENT 
653 0 |a ANIMAL MODEL 
653 0 |a ANIMAL TISSUE 
653 0 |a APOPTOSIS 
653 0 |a AREGB GENE 
653 0 |a ARHGAP21 GENE 
653 0 |a BOVINE TUBERCULOSIS 
653 0 |a CATTLE DISEASE 
653 0 |a CCL2 GENE 
653 0 |a CCL8 GENE 
653 0 |a CCR1 GENE 
653 0 |a CD14 GENE 
653 0 |a CELL TRANSPORT 
653 0 |a CSF3R GENE 
653 0 |a CXCL10 GENE 
653 0 |a CXCL9 GENE 
653 0 |a CXCR2 GENE 
653 0 |a DEFBIO GENE 
653 0 |a DOWN REGULATION 
653 0 |a ECM1 GENE 
653 0 |a ENDOCYTOSIS 
653 0 |a FCAR GENE 
653 0 |a FCGR1A GENE 
653 0 |a FYVE GENE 
653 0 |a GENE 
653 0 |a GENE EXPRESSION PROFILING 
653 0 |a GENETIC VARIABILITY 
653 0 |a GZMA GENE 
653 0 |a HAS2 GENE 
653 0 |a IL 18 GENE 
653 0 |a IL 1R GENE 
653 0 |a IL 6 GENE 
653 0 |a IL13RA1 GENE 
653 0 |a IL1R1 GENE 
653 0 |a IL1RN GENE 
653 0 |a IL22 GENE 
653 0 |a IMMUNE RESPONSE 
653 0 |a INFLAMMATION 
653 0 |a LOC508666 GENE 
653 0 |a LOC533894 GENE 
653 0 |a MAPK13 GENE 
653 0 |a MAPK14 GENE 
653 0 |a MICROARRAY ANALYSIS 
653 0 |a MMP19 GENE 
653 0 |a MMP3 GENE 
653 0 |a MMP9 GENE 
653 0 |a MRC1 GENE 
653 0 |a MYCOBACTERIUM BOVIS 
653 0 |a MYD88 GENE 
653 0 |a NFAM1 GENE 
653 0 |a NONHUMAN 
653 0 |a NRP1 GENE 
653 0 |a PERIPHERAL BLOOD MONONUCLEAR CELL 
653 0 |a PF4 GENE 
653 0 |a PLAUR GENE 
653 0 |a PROTEIN DEGRADATION 
653 0 |a PYCARD GENE 
653 0 |a QUANTITATIVE ANALYSIS 
653 0 |a RETN GENE 
653 0 |a REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION 
653 0 |a S100A9 GENE 
653 0 |a SASH1 GENE 
653 0 |a SH3BP4 GENE 
653 0 |a TGFBI GENE 
653 0 |a TGM3 GENE 
653 0 |a THBS1 GENE 
653 0 |a TREM1 GENE 
653 0 |a TYROBP GENE 
653 0 |a UPREGULATION 
653 0 |a VLDLR GENE 
653 0 |a ANIMALS 
653 0 |a BIOLOGICAL MARKERS 
653 0 |a CATTLE 
653 0 |a ENDOCYTOSIS 
653 0 |a GENE EXPRESSION PROFILING 
653 0 |a GENE EXPRESSION REGULATION, BACTERIAL 
653 0 |a INFLAMMATION 
653 0 |a LEUKOCYTES, MONONUCLEAR 
653 0 |a MODELS, GENETIC 
653 0 |a MYCOBACTERIUM BOVIS 
653 0 |a OLIGONUCLEOTIDE ARRAY SEQUENCE ANALYSIS 
653 0 |a TRANSCRIPTION, GENETIC 
653 0 |a TUBERCULOSIS, BOVINE 
653 0 |a BOS 
653 0 |a BOVINAE 
653 0 |a MYCOBACTERIUM BOVIS 
700 1 |9 70699  |a Blanco, Federico Carlos 
700 |a Soria, M. A.  |9 48353 
700 1 |9 48056  |a Bianco, María Verónica 
700 1 |9 48059  |a Bigi, Fabiana 
773 |t Plos One  |g Vol.7, no.7 (2012), e41066, (7 p.) 
856 |u http://ri.agro.uba.ar/files/download/articulo/2012Blanco.pdf  |i En internet  |q application/pdf  |f 2012Blanco  |x MIGRADOS2018 
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900 |a 10.1371/journal.pone.0041066 
900 |a ^tTranscriptional response of peripheral blood mononuclear cells from cattle infected with Mycobacterium bovis 
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900 |a ^aSoria^bM. 
900 |a ^aBianco^bM.V. 
900 |a ^aBigi^bF. 
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900 |a ^aSoria^bM. A. 
900 |a ^aBianco^bM. V. 
900 |a ^aBigi^bF. 
900 |a ^aBlanco, F.C.^tInstituto de Biotecnología-CICVyA, Instituto Nacional de Tecnología Agropecuaria, Hurlingham, Buenos Aires, Argentina 
900 |a ^aSoria, M.^tAgricultural Microbiology, School of Agronomy, Buenos Aires University, INBA-Consejo Nacional de Investigaciones Científicas y Técnicas, Ciudad de Buenos Aires, Argentina 
900 |a ^aBianco, M.V.^t 
900 |a ^aBigi, F.^t 
900 |a ^tPLoS ONE^cPLoS ONE 
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900 |a Vol. 7, no. 7 
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900 |a BIOLOGICAL MARKER 
900 |a CALGRANULIN B 
900 |a CD14 ANTIGEN 
900 |a CHEMOKINE RECEPTOR CXCR2 
900 |a CXCL9 CHEMOKINE 
900 |a FC FRAGMENT IMMUNOGLOBULIN A RECEPTOR 
900 |a GELATINASE B 
900 |a HYALURONAN SYNTHASE 2 
900 |a INTERLEUKIN 1 RECEPTOR 
900 |a INTERLEUKIN 13 RECEPTOR ALPHA1 
900 |a INTERLEUKIN 22 
900 |a LOW DENSITY LIPOPROTEIN RECEPTOR RELATED PROTEIN 
900 |a MANNOSE RECEPTOR C TYPE 1 
900 |a MATRIX METALLOPROTEINASE 19 
900 |a MONOCYTE CHEMOTACTIC PROTEIN 2 
900 |a NEUROPILIN 1 
900 |a RESISTIN 
900 |a RNA 
900 |a SH3 DOMAIN BINDING PROTEIN 4 
900 |a THROMBOCYTE FACTOR 4 
900 |a TRANSCRIPTION FACTOR 
900 |a TRANSCRIPTION FACTOR FYVE 
900 |a TRANSCRIPTION FACTOR GZMA 
900 |a TRANSCRIPTION FACTOR THBS1 
900 |a TRANSFORMING GROWTH FACTOR BETA 
900 |a TRANSGLUTAMINASE 3 
900 |a UNCLASSIFIED DRUG 
900 |a UROKINASE RECEPTOR 
900 |a VERY LOW DENSITY LIPOPROTEIN RECEPTOR 
900 |a ADAM8 GENE 
900 |a ADAMTSL4 GENE 
900 |a ANIMAL CELL 
900 |a ANIMAL EXPERIMENT 
900 |a ANIMAL MODEL 
900 |a ANIMAL TISSUE 
900 |a APOPTOSIS 
900 |a AREGB GENE 
900 |a ARHGAP21 GENE 
900 |a BOVINE TUBERCULOSIS 
900 |a CATTLE DISEASE 
900 |a CCL2 GENE 
900 |a CCL8 GENE 
900 |a CCR1 GENE 
900 |a CD14 GENE 
900 |a CELL TRANSPORT 
900 |a CSF3R GENE 
900 |a CXCL10 GENE 
900 |a CXCL9 GENE 
900 |a CXCR2 GENE 
900 |a DEFBIO GENE 
900 |a DOWN REGULATION 
900 |a ECM1 GENE 
900 |a ENDOCYTOSIS 
900 |a FCAR GENE 
900 |a FCGR1A GENE 
900 |a FYVE GENE 
900 |a GENE 
900 |a GENE EXPRESSION PROFILING 
900 |a GENETIC VARIABILITY 
900 |a GZMA GENE 
900 |a HAS2 GENE 
900 |a IL 18 GENE 
900 |a IL 1R GENE 
900 |a IL 6 GENE 
900 |a IL13RA1 GENE 
900 |a IL1R1 GENE 
900 |a IL1RN GENE 
900 |a IL22 GENE 
900 |a IMMUNE RESPONSE 
900 |a INFLAMMATION 
900 |a LOC508666 GENE 
900 |a LOC533894 GENE 
900 |a MAPK13 GENE 
900 |a MAPK14 GENE 
900 |a MICROARRAY ANALYSIS 
900 |a MMP19 GENE 
900 |a MMP3 GENE 
900 |a MMP9 GENE 
900 |a MRC1 GENE 
900 |a MYCOBACTERIUM BOVIS 
900 |a MYD88 GENE 
900 |a NFAM1 GENE 
900 |a NONHUMAN 
900 |a NRP1 GENE 
900 |a PERIPHERAL BLOOD MONONUCLEAR CELL 
900 |a PF4 GENE 
900 |a PLAUR GENE 
900 |a PROTEIN DEGRADATION 
900 |a PYCARD GENE 
900 |a QUANTITATIVE ANALYSIS 
900 |a RETN GENE 
900 |a REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION 
900 |a S100A9 GENE 
900 |a SASH1 GENE 
900 |a SH3BP4 GENE 
900 |a TGFBI GENE 
900 |a TGM3 GENE 
900 |a THBS1 GENE 
900 |a TREM1 GENE 
900 |a TYROBP GENE 
900 |a UPREGULATION 
900 |a VLDLR GENE 
900 |a ANIMALS 
900 |a BIOLOGICAL MARKERS 
900 |a CATTLE 
900 |a ENDOCYTOSIS 
900 |a GENE EXPRESSION PROFILING 
900 |a GENE EXPRESSION REGULATION, BACTERIAL 
900 |a INFLAMMATION 
900 |a LEUKOCYTES, MONONUCLEAR 
900 |a MODELS, GENETIC 
900 |a MYCOBACTERIUM BOVIS 
900 |a OLIGONUCLEOTIDE ARRAY SEQUENCE ANALYSIS 
900 |a TRANSCRIPTION, GENETIC 
900 |a TUBERCULOSIS, BOVINE 
900 |a BOS 
900 |a BOVINAE 
900 |a MYCOBACTERIUM BOVIS 
900 |a Mycobacterium bovis is the causative agent of most cases of bovine tuberculosis. The identification of bTB biomarkers in specific stages of the disease will contribute to a better understanding of the immunopathology associated with tuberculosis and will enable their use in disease diagnosis and prognosis. The aim of this study was to evaluate the gene expression profile induced after specific stimulation of bovine peripheral blood mononuclear cells from cattle infected with M. bovis using the Affymetrix® GeneChip® Bovine Genome Array. A total of 172 genes showed differential expression profile that was statistically significant with log2-fold change grather than 2.5 and less than -2.5. Twenty-four out of these genes were upregulated and 148 were downregulated in bovine peripheral blood mononuclear cells of M. bovis-infected cattle. The highest differentially-expressed genes were related to immune and inflammatory responses, apoptosis, endocytosis, cellular trafficking and genes encoding proteins involved in cellular matrix degradation. Microarray results were confirmed in another group of infected cattle by RT-qPCR for the CD14, IL-1R, THBS1, MMP9 and FYVE genes. This study confirms previous findings that have shown that M. bovis infection in cattle results in the downregulation of immune response-related genes. Moreover, it validates the use of microarray platforms in combination with RT-qPCR to identify biomarkers of bovine tuberculosis. In addition, we propose CD14, IL-1R, THBS1, MMP9 and FYVE as potential biomarkers of bovine tuberculosis. 
900 |a 7 
900 |a 7 
900 |a 2012 
900 |a ^cH 
900 |a AAG 
900 |a AGROVOC 
900 |a 2012Blanco 
900 |a AAG 
900 |a http://ri.agro.uba.ar/files/download/articulo/2012Blanco.pdf 
900 |a 2012Blanco.pdf 
900 |a http://www.plos.org/ 
900 |a http://www.scopus.com/inward/record.url?eid=2-s2.0-84863995550&partnerID=40&md5=191725d48ec2bd9ed20581d1ccff829a 
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