The effect of nitrogen enrichment on C1 - cycling microorganisms and methane flux in salt marsh sediments

Methane [CH4] flux from ecosystems is driven by C1-cycling microorganisms-the methanogens and the methylotrophs. Little is understood about what regulates these communities, complicating predictions about how global change drivers such as nitrogen enrichment will affect methane cycling. Using a nitr...

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Otros Autores: Irvine, Irina C., Vivanco, Lucía, Bentley, Peris N., Martiny, Jennifer B. H.
Formato: Artículo
Lenguaje:Español
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Acceso en línea:http://ri.agro.uba.ar/files/download/articulo/2012Irvine.pdf
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245 1 0 |a The effect of nitrogen enrichment on C1 - cycling microorganisms and methane flux in salt marsh sediments 
520 |a Methane [CH4] flux from ecosystems is driven by C1-cycling microorganisms-the methanogens and the methylotrophs. Little is understood about what regulates these communities, complicating predictions about how global change drivers such as nitrogen enrichment will affect methane cycling. Using a nitrogen addition gradient experiment in three Southern California salt marshes, we show that sediment CH4 flux increased linearly with increasing nitrogen addition [1.23 ug CH4 m-2 day-1 for each gNm-2year-1 applied] after 7 months of fertilization. To test the reason behind this increased CH4 flux, we conducted a microcosm experiment altering both nitrogen and carbon availability under aerobic and anaerobic conditions. Methanogenesis appeared to be both nitrogen and carbon [acetate] limited. N and C each increased methanogenesis by 18 percent, and together by 44 percent. In contrast, methanotrophy was stimulated by carbon [methane] addition [830 percent], but was unchanged by nitrogen addition. Sequence analysis of the sediment methylotroph community with the methanol dehydrogenase gene [mxaF] revealed three distinct clades that fall outside of known lineages. However, in agreement with the microcosm results, methylotroph abundance [assayed by qPCR] and composition [assayed by terminal restriction fragment length polymorphism analysis] did not vary across the experimental nitrogen gradient in the field. Together, these results suggest that nitrogen enrichment to salt marsh sediments increases methane flux by stimulating the methanogen community. 
650 |2 Agrovoc  |9 26 
653 0 |a ACETATE 
653 0 |a METHANE FLUX 
653 0 |a METHANOGENESIS 
653 0 |a METHANOTROPHY 
653 0 |a METHYLOTROPHY 
653 0 |a MXAF 
653 0 |a NITROGEN GRADIENT 
653 0 |a NUTRIENT LIMITATION 
700 1 |a Irvine, Irina C.  |9 70381 
700 1 |a Vivanco, Lucía  |9 30802 
700 1 |a Bentley, Peris N.  |9 71926 
700 1 |a Martiny, Jennifer B. H.  |9 70382 
773 |t Frontiers in Microbiology  |g Vol.3 (2012), art.90, 10 p., tbls., grafs. 
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