Effect of bitter compounds on amylase secretion in murine submandibular glands: Signaling pathway mechanisms
Background: Amylase is synthesized in submandibular glands (SMG) and released into the oral cavity to degrade carbohydrates in the mouth. Bitter taste receptors (T2R) belong to the G-protein coupled receptor (GPCR) family and are expressed in the taste cells and also in the digestive tract. Methods:...
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2011
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| 003 | AR-BaUEN | ||
| 005 | 20230518204008.0 | ||
| 008 | 190411s2011 xx ||||fo|||| 00| 0 eng|d | ||
| 024 | 7 | |2 scopus |a 2-s2.0-80054998442 | |
| 024 | 7 | |2 cas |a amylase, 9000-90-2, 9000-92-4, 9001-19-8; cyclic AMP, 60-92-4; cycloheximide, 642-81-9, 66-81-9; denatonium benzoate, 3734-33-6; inositol phosphate, 15421-51-9; theophylline, 58-55-9, 5967-84-0, 8055-07-0, 8061-56-1, 99007-19-9; Amylases, 3.2.1.-; Cyclic AMP, 60-92-4 | |
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| 100 | 1 | |a Dasso, M. | |
| 245 | 1 | 0 | |a Effect of bitter compounds on amylase secretion in murine submandibular glands: Signaling pathway mechanisms |
| 260 | |c 2011 | ||
| 270 | 1 | 0 | |m Sales, M.E.; Centro de Estudios Farmacológicos y Botánicos (CEFYBO)-CONICET, Facultad de Medicina, Universidad de Buenos Aires, Paraguay 2155 piso 16 sector der. CP 1121, Buenos Aires, Argentina; email: msales@fmed.uba.ar |
| 506 | |2 openaire |e Política editorial | ||
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| 520 | 3 | |a Background: Amylase is synthesized in submandibular glands (SMG) and released into the oral cavity to degrade carbohydrates in the mouth. Bitter taste receptors (T2R) belong to the G-protein coupled receptor (GPCR) family and are expressed in the taste cells and also in the digestive tract. Methods: The activity of amylase secreted by murine SMG was measured, detecting maltose by Bernfeld's method. Amylase and T2R6 were detected by imunohistochemistry and Western blot. The expression of Ggustducin, Gi, and phospholipase Cβ2 was also studied by Western blot. cAMP levels were measured by radioimmunoassay and inositol monophosphate production was quantified by ELISA. Results: Theophylline, denatonium and cycloheximide exerted a dose-dependent inhibition on amylase secretion. This effect was reverted by preincubating SMG with an anti-Gαi antibody. cAMP production was increased by the same compounds, an effect that was also abrogated by an anti-Gαi antibody. Bitter compounds reduced inositol monophosphate formation in SMG and H-89, a protein kinase A inhibitor, reverted this action, revealing that this protein kinase down regulates phospholipase C activity. General significance: We demonstrated that theophylline, denatonium and cycloheximide inhibit salivary amylase secretion, activating an intracellular signaling pathway that involves cAMP and phospholipase C, that cross talks via protein kinase A. © 2011 Elsevier B.V. |l eng | |
| 536 | |a Detalles de la financiación: National Science and Technology Development Agency | ||
| 536 | |a Detalles de la financiación: Universidad de Buenos Aires, UBACyT M064 | ||
| 536 | |a Detalles de la financiación: Agencia Nacional de Promoción Científica y Tecnológica, PICT 2006–485 | ||
| 536 | |a Detalles de la financiación: The authors wish to thank Drs. Maria P. Cecchini, Flavia Merigo and Andrea Sbarbati from the Dipartimento di Scienze Morfologico-Biomediche, Sezione di Anatomia e Istologia, Universitá degli Studi di Verona, Italy, where the immunohistochemistry slides were developed. Also our special thanks to Mrs. María Esther Castro for her skillful technical assistance in mice surgical procedures and Drs. Eulalia de la Torre, Alejandro Español and Ganna Dmytrenko for their help and observations on the different experimental techniques employed. This research was funded in part by Fundación Conamed; University of Buenos Aires grant UBACyT M064 and the National Agency for the Promotion of Science and Technology (ANPCyT) grant PICT 2006–485 . | ||
| 593 | |a Centro de Estudios Farmacológicos y Botánicos (CEFYBO)-CONICET, Facultad de Medicina, Universidad de Buenos Aires, Paraguay 2155 piso 16 sector der. CP 1121, Buenos Aires, Argentina | ||
| 593 | |a Instituto de Biología y Medicina Experimental (IBYME)-CONICET, Buenos Aires, Argentina | ||
| 593 | |a Dpto. de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina | ||
| 593 | |a Cátedra de Farmacología, Facultad de Medicina, Universidad de Buenos Aires, Buenos Aires, Argentina | ||
| 690 | 1 | 0 | |a AMYLASE |
| 690 | 1 | 0 | |a BITTER AGONIST |
| 690 | 1 | 0 | |a CAMP |
| 690 | 1 | 0 | |a GI PROTEIN |
| 690 | 1 | 0 | |a INOSITOL MONOPHOSPHATE |
| 690 | 1 | 0 | |a PROTEIN KINASE A |
| 690 | 1 | 0 | |a AMYLASE |
| 690 | 1 | 0 | |a CYCLIC AMP |
| 690 | 1 | 0 | |a CYCLIC AMP DEPENDENT PROTEIN KINASE |
| 690 | 1 | 0 | |a CYCLOHEXIMIDE |
| 690 | 1 | 0 | |a DENATONIUM BENZOATE |
| 690 | 1 | 0 | |a GUANINE NUCLEOTIDE BINDING PROTEIN |
| 690 | 1 | 0 | |a GUSTDUCIN |
| 690 | 1 | 0 | |a INHIBITORY GUANINE NUCLEOTIDE BINDING PROTEIN |
| 690 | 1 | 0 | |a INOSITOL PHOSPHATE |
| 690 | 1 | 0 | |a PHOSPHOLIPASE C BETA2 |
| 690 | 1 | 0 | |a THEOPHYLLINE |
| 690 | 1 | 0 | |a UNCLASSIFIED DRUG |
| 690 | 1 | 0 | |a ANIMAL TISSUE |
| 690 | 1 | 0 | |a ARTICLE |
| 690 | 1 | 0 | |a BITTER TASTE |
| 690 | 1 | 0 | |a CONTROLLED STUDY |
| 690 | 1 | 0 | |a ENZYME ACTIVITY |
| 690 | 1 | 0 | |a ENZYME INHIBITION |
| 690 | 1 | 0 | |a ENZYME LINKED IMMUNOSORBENT ASSAY |
| 690 | 1 | 0 | |a ENZYME LOCALIZATION |
| 690 | 1 | 0 | |a ENZYME RELEASE |
| 690 | 1 | 0 | |a IMMUNOHISTOCHEMISTRY |
| 690 | 1 | 0 | |a MOUSE |
| 690 | 1 | 0 | |a NONHUMAN |
| 690 | 1 | 0 | |a PRIORITY JOURNAL |
| 690 | 1 | 0 | |a PROTEIN EXPRESSION |
| 690 | 1 | 0 | |a PROTEIN FUNCTION |
| 690 | 1 | 0 | |a QUANTITATIVE ANALYSIS |
| 690 | 1 | 0 | |a RADIOIMMUNOASSAY |
| 690 | 1 | 0 | |a SIGNAL TRANSDUCTION |
| 690 | 1 | 0 | |a SUBMANDIBULAR GLAND |
| 690 | 1 | 0 | |a TASTE BUD |
| 690 | 1 | 0 | |a WESTERN BLOTTING |
| 690 | 1 | 0 | |a AMYLASES |
| 690 | 1 | 0 | |a ANIMALS |
| 690 | 1 | 0 | |a BLOTTING, WESTERN |
| 690 | 1 | 0 | |a CYCLIC AMP |
| 690 | 1 | 0 | |a IMMUNOHISTOCHEMISTRY |
| 690 | 1 | 0 | |a MICE |
| 690 | 1 | 0 | |a MICE, INBRED BALB C |
| 690 | 1 | 0 | |a SIGNAL TRANSDUCTION |
| 690 | 1 | 0 | |a SUBMANDIBULAR GLAND |
| 690 | 1 | 0 | |a MURINAE |
| 700 | 1 | |a Pagotto, R. | |
| 700 | 1 | |a Pignataro, O.P. | |
| 700 | 1 | |a Diez, R.A. | |
| 700 | 1 | |a Sales, M.E. | |
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