DNA supercoiling and thermal regulation of unsaturated fatty acid synthesis in Bacillus subtilis

Bacillus subtilis growing at 37° C synthesizes, almost exclusively, saturated fatty acids. However, when a culture growing at 37°C is transferred to 20°C, the synthesis of unsaturated fatty acids is induced. The addition of the DNA gyrase inhibitor novobiocin specifically prevented the induction of...

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Autor principal: Grau, R.
Otros Autores: Gardiol, D., Glikin, G.C, De Mendoza, D.
Formato: Capítulo de libro
Lenguaje:Inglés
Publicado: 1994
Acceso en línea:Registro en Scopus
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024 7 |2 scopus  |a 2-s2.0-0028264505 
024 7 |2 cas  |a Bacterial Proteins; DNA Topoisomerases, Type II, EC 5.99.1.3; DNA, Bacterial; DNA, Superhelical; Fatty Acids, Unsaturated; Novobiocin, 303-81-1 
040 |a Scopus  |b spa  |c AR-BaUEN  |d AR-BaUEN 
100 1 |a Grau, R. 
245 1 0 |a DNA supercoiling and thermal regulation of unsaturated fatty acid synthesis in Bacillus subtilis 
260 |c 1994 
270 1 0 |m de Mendoza, D. 
506 |2 openaire  |e Política editorial 
504 |a Anagnostopoulos, C., Piggot, P.J., Hoch, J.A., The genetic map of Bacillus subtilis (1993) Bacillus subtilis and Other Gram Positive Bacteria: Physiology, Biochemistry and Molecular Biology, pp. 425-463. , Losick, R., Sonenshein, A.L., Hoch, J.A., (eds). Washington, D.C.:, American Society for Microbiology 
504 |a Burrington, M.G., Morgan, A.R., The purification and characterization of a DNA nicking‐closing enzyme from Bacillus megaterium (1978) Can J Biochem, 56, pp. 123-128 
504 |a Depew, R.E., Wang, J.C., Conformational fluctuations of DNA helix (1975) Proc Natl Acad Sci USA, 72, pp. 4275-4279 
504 |a Dorman, C.J., Barr, G.C., Ni Bhriain, N., Higgins, C.F., DNA supercoiling and the anaerobic and growth phase regulation of tonB gene expression (1988) J Bacteriol, 170, pp. 2816-2826 
504 |a Dorman, C.J., Ni Bhriain, N., Higgins, C.F., DNA supercoiling and environmental regulation in virulence gene expression in Shigella flexneri (1990) Nature, 344, pp. 789-792 
504 |a Drlica, K., Biology of bacterial deoxyribonucleic acid topoisomerases (1984) Microbiol Rev, 48, pp. 273-289 
504 |a Drlica, K., Bacterial topoisomerases and the control of DNA supercoiling (1990) Trends Genet, 12, pp. 433-437 
504 |a Drlica, K., Control of bacterial DNA supercoiling (1992) Mol Microbiol, 6, pp. 425-433 
504 |a Fujii, D.K., Fulco, A.J., Biosynthesis of unsaturated fatty acids in Bacilli: hyperinduction and modulation of desaturase synthesis (1977) J Biol Chem, 252, pp. 3660-3670 
504 |a Fulco, A.J., Fatty acid metabolism in bacteria (1983) Progr Lipid Res, 22, pp. 133-160 
504 |a Gardiol, D., Gramajo, H., Hirschbein, L., de Mendoza, D., Application of the mini‐Mu phage for the isolation of lac transcriptional fusions in Bacillus subtilis genes (1993) Gene, 123, pp. 39-44 
504 |a Gellert, M., O'Dea, M.H., Itoh, T., Tomizawa, J., Novobiocin and coumarmycin inhibit DNA supercoiling catalyzed by DNA gyrase (1976) Proc Natl Acad Sci USA, 73, pp. 4474-4478 
504 |a Goldstein, E., Drlica, K., Regulation of bacterial DNA supercoiling: plasmid linking vary with growth temperature (1984) Proc Natl Acad Sci USA, 81, pp. 4046-4050 
504 |a Grau, R., de Mendoza, D., Regulation of the synthesis of unsaturated fatty acids by growth temperature in Bacillus subtilis (1993) Mol Microbiol, 8, pp. 535-542 
504 |a Hartford, O.M., Dowds, B.C.A., Cloning and characterization of genes induced by hydrogen peroxide in Bacillus subtilis (1992) J Gen Microbiol, 138, pp. 2061-2068 
504 |a Higgins, C.F., Dorman, C.J., Stirling, D.A., Waddell, L., Booth, I.R., May, G., Bremer, E., A physiological role of DNA supercoiling in the osmotic regulation of gene expression in Salmonella typhimurium and Escherichia coli (1988) Cell., 52, pp. 569-584 
504 |a Higgins, C.F., Dorman, C.J., Ni Bhriain, N., Environmental influences on DNA supercoiling: a novel mechanism for the regulation of gene expression (1990) The Bacterial Chromosome, pp. 421-432. , Drlica, K., Riley, M., (eds). Washington, D.C.:, American Society for Microbiology 
504 |a Jones, P.G., Krah, R., Tafuri, S.R., Wolffe, A., DNA gyrase, CS7.4, and the cold shock response in Escherichia coli (1992) J Bacteriol, 174, pp. 5798-5802 
504 |a Lampe, M.F., Bott, K.F., Genetic and organization of the cloned gyrA and gyrB genes of Bacillus subtilis (1985) J Bacteriol, 162, pp. 78-84 
504 |a de Mendoza, D., Cronan, J.E., Jr, Thermal regulation of membrane lipid fluidity in bacteria (1983) Trends Biochem Sci, 8, pp. 49-52 
504 |a de Mendoza, D., Grau, R., Cronan, J.E., Jr, Biosynthesis and function of membrane lipids (1993) Bacillus subtilis and Other Gram Positive Bacteria: Physiology, Biochemistry and Molecular Biology, pp. 411-425. , Losick, R., Sonenshein, A.L., Hoch, J.A., (eds). Washington, D.C.:, American Society for Microbiology 
504 |a de Mendoza, D., Klages‐Ulrich, A., Cronan, J.E., Jr, Thermal regulation of membrane fluidity in Escherichia coli. Effects of overproduction of β‐keto‐acyl‐acyl‐carrier protein synthase I (1983) J Biol Chem, 258, pp. 2098-2101 
504 |a Mizushima, T., Natori, S., Sekimizu, K., Inhibition of Escherichia coli DNA topoisomerase I activity by phospholipids (1992) Biochem J, 285, pp. 503-506 
504 |a Nicholson, W.L., Setlow, P., Dramatic increase in negative superhelicity of plasmid DNA in the forespore compartment of sporulating cells of Bacillus subtilis (1990) J Bacteriol, 172, pp. 7-14 
504 |a Pruss, G.J., DNA topoisomarase I mutants: increased heterogeneity in linking number and other replicon‐dependant changes in DNA supercoiling (1985) J Mol Biol, 185, pp. 51-63 
504 |a Pruss, G.I., Drlica, K., DNA supercoiling and prokaryotic transcription (1989) Cell, 56, pp. 521-523 
504 |a Spizizen, J., Transformation of biochemically deficient strains of Bacillus subtilis by deoxyribonucleate (1958) Proceedings of the National Academy of Sciences, 44, pp. 1072-1078 
504 |a Sugino, A., Bott, K.F., Bacillus subtilis deoxyribonucleic acid gyrase (1980) J Bacteriol, 141, pp. 1331-1339 
504 |a Tovar‐Rojo, F., Setlow, P., Effects of mutant small, acid‐soluble spore proteins from Bacillus subtilis on DNA in vivo and in vitro (1991) J Bacteriol, 173, pp. 4827-4835 
504 |a Wang, J.Y., Syvanen, M., DNA twist as a transcriptional sensor for environmental changes (1992) Mol Microbiol, 6, pp. 1861-1866 
504 |a Willimsky, G., Bang, H., Fischer, G., Marahiel, M.A., Characterization of cspB, a Bacillus subtilis inducible cold shock gene affecting cell viability at low temperatures (1992) J Bacteriol, 174, pp. 6326-6335 
520 3 |a Bacillus subtilis growing at 37° C synthesizes, almost exclusively, saturated fatty acids. However, when a culture growing at 37°C is transferred to 20°C, the synthesis of unsaturated fatty acids is induced. The addition of the DNA gyrase inhibitor novobiocin specifically prevented the induction of unsaturated fatty acid synthesis at 20° C. Furthermore, it was determined that plasmid DNA isolated from cells growing at 20°C was significantly more negatively supercoiled than the equivalent DNA isolated from cells growing at 37°C. The overall results agree with the hypothesis that an increase in DNA supercoiling associated with a temperature downshift could regulate the unsaturated fatty acids synthesis in B. subtilis. Copyright © 1994, Wiley Blackwell. All rights reserved  |l eng 
593 |a Departamento de Microbiologiá, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Suipacha 531, Rosario, 2000, Argentina 
593 |a Instituto de Investigaciones en Ingenieriá Genética y Biologiá Molecular, Consejo Nacional de Investigaciones Científicas y Técnicas, y Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Vuelta de Obligado 2490, Buenos Aires, 1428, Argentina 
690 1 0 |a NOVOBIOCIN 
690 1 0 |a UNSATURATED FATTY ACID 
690 1 0 |a ARTICLE 
690 1 0 |a BACILLUS SUBTILIS 
690 1 0 |a DNA SUPERCOILING 
690 1 0 |a FATTY ACID SYNTHESIS 
690 1 0 |a NONHUMAN 
690 1 0 |a PRIORITY JOURNAL 
690 1 0 |a TEMPERATURE 
690 1 0 |a BACILLUS SUBTILIS 
690 1 0 |a BACTERIAL PROTEINS 
690 1 0 |a DNA TOPOISOMERASES, TYPE II 
690 1 0 |a DNA, BACTERIAL 
690 1 0 |a DNA, SUPERHELICAL 
690 1 0 |a FATTY ACIDS, UNSATURATED 
690 1 0 |a GENE EXPRESSION REGULATION, BACTERIAL 
690 1 0 |a NOVOBIOCIN 
690 1 0 |a SUPPORT, NON-U.S. GOV'T 
690 1 0 |a TEMPERATURE 
690 1 0 |a BACILLUS SUBTILIS 
700 1 |a Gardiol, D. 
700 1 |a Glikin, G.C. 
700 1 |a De Mendoza, D. 
773 0 |d 1994  |g v. 11  |h pp. 933-941  |k n. 5  |p Mol. Microbiol.  |x 0950382X  |w (AR-BaUEN)CENRE-6160  |t Molecular Microbiology 
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856 4 0 |u https://doi.org/10.1111/j.1365-2958.1994.tb00372.x  |y DOI 
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