Differential effect of αdifluoromethyl‐ornithine on the proliferation of balb 3T3 and chemically transformed 3T3 cells

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In order to study the effect of polyamine depletion on growth and proliferation of untransformed and chemically transformed cells, α‐difluoromethyl‐ornithine (DFMO) was added to cultures of 3T3 cells and their benzo[a]pyrene derivative BP‐3T3. Both types of cells stopped their proliferation after 72...

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Detalles Bibliográficos
Autor principal: Medrano, E.E
Otros Autores: Goldemberg, S.H, Algranati, I.D
Formato: Capítulo de libro
Lenguaje:Inglés
Publicado: 1983
Acceso en línea:Registro en Scopus
DOI
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Registro en la Biblioteca Digital
Aporte de:Registro referencial: Solicitar el recurso aquí
Biblioteca Central Dr. Luis F. Leloir (FCEN) de Universidad de Buenos Aires
LEADER 09770caa a22010817a 4500
001 PAPER-12169
003 AR-BaUEN
005 20230518204222.0
008 190411s1983 xx ||||fo|||| 00| 0 eng|d
024 7 |2 scopus  |a 2-s2.0-0021016752 
024 7 |2 cas  |a benzo[a]pyrene, 50-32-8; eflornithine, 67037-37-0, 70052-12-9; putrescine, 110-60-1, 333-93-7; spermidine, 124-20-9, 334-50-9; Benzo(a)pyrene, 50-32-8; Benzopyrenes; Carcinogens; Eflornithine, 70052-12-9; Ornithine Decarboxylase, EC 4.1.1.17; Ornithine, 7006-33-9; Putrescine, 110-60-1 
040 |a Scopus  |b spa  |c AR-BaUEN  |d AR-BaUEN 
100 1 |a Medrano, E.E. 
245 1 0 |a Differential effect of αdifluoromethyl‐ornithine on the proliferation of balb 3T3 and chemically transformed 3T3 cells 
260 |c 1983 
270 1 0 |m Algranati, I.D.; Instituto de Investigaciones Bioquimicas "Fundacion Campomar" and Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Obligado 2490, Buenos Aires, 1428, Argentina 
506 |2 openaire  |e Política editorial 
504 |a Abraham, A.K., Pihl, A., Role of polyamines in macromolecular synthesis (1981) Trends Biochem. Sci., 6, pp. 106-107 
504 |a Algranati, I.D., Goldemberg, S.H., Polyamines and their role in protein synthesis (1977) Trends Biochem. Sci., 2, pp. 272-274 
504 |a Baserga, R., (1976) Multiplication and Division in Mammalian Cells, , Marcel Dekker, Inc., New York 
504 |a Bonner, W.M., Laskey, R.A., A film detection method for tritium‐labelled proteins and nucleic acids in polyacrylamide gels (1974) Eur. J. Biochem., 46, pp. 83-88 
504 |a Brown, K.D., Holley, R.W., Epidermal growth factor and the control of proliferation of Balb 3T3 and benzo[a]pyrene‐transformed Balb 3T3 cells (1979) J. Cell. Physiol., 100, pp. 139-146 
504 |a Campisi, J., Medrano, E.E., Cell cycle perturbations in normal and transformed fibroblasts caused by detachment from the substratum (1983) J. Cell. Physiol., 114, pp. 53-60 
504 |a Dion, A.S., Herbst, E.J., Polyamine changes during development of Drosophila melanogaster (1970) Annals of the New York Academy of Sciences, 171, pp. 723-734 
504 |a Dubrow, R., Riddle, V.G.H., Pardee, A.B., Different responses to drugs and serum of cells transformed by various means (1979) Cancer Res., 39, pp. 2718-2726 
504 |a Heby, O., Andersson, G., Gray, J.W., Marton, L.J., Accumulation of rat brain tumor cells in G1 and of Ehrlich ascites tumor cells in G2 by partial deprivation of cellular polyamine content (1978) Advances in Polyamine Research, 1, pp. 127-131. , R. A. Campbell, D. R. Morris, D. Bartos, G. Doyle Daves, F. Bartos, Raven Press, New York 
504 |a Heby, O., Jänne, J., Polyamine antimetabolites: Biochemistry, specificity, and biological effects of inhibitors of polyamine synthesis (1981) Polyamines in Biology and Medicine, pp. 243-310. , D. R. Morris, L. J. Marton, Marcel Dekker, New York 
504 |a Heidelberger, C., Chemical carcinogenesis (1975) Annu. Rev. Biochem., 44, pp. 79-121 
504 |a Holley, R.W., Baldwin, J.H., Kiernan, J.A., Messmer, T.O., Control of growth of benzo[a]pyrene‐transformed 3T3 cells (1976) Proc. Natl. Acad. Sci. U.S.A., 73, pp. 3229-3232 
504 |a Jänne, J., Pösö, H., Raina, A., Polyamines in rapid growth and cancer (1978) Biochim Biophys Acta, 473, pp. 241-293 
504 |a Kameji, T., Murakami, Y., Fujita, K., Noguchi, T., Hayashi, S., Rat liver ornithine decarboxylase purification and some immunochemical studies (1981) Med. Biol., 59, pp. 296-299 
504 |a Laemmli, U.K., Cleavage of structural proteins during the assembly of the head of bacteriophage T4 (1970) Nature, 227, pp. 680-685 
504 |a Lawrence, F., Bachrach, V., Robert‐Géro, M., Effect of 5′‐deoxy‐5′‐S‐isobutylthioadenosine on putrescine uptake and polyamine biosynthesis by chick embryo fibroblasts (1982) Biochem. J., 204, pp. 853-859 
504 |a Mamont, P.S., Böhlen, P., McCann, P.P., Bey, P., Schuber, F., Tardif, C., α‐Methyl ornithine, a potent competitive inhibitor of ornithine decarboxylase, blocks proliferation of rat hepatome cells in culture (1976) Proc. Natl. Acad. Sci. U.S.A., 73, pp. 1626-1630 
504 |a Mamont, P.S., Duchesne, M.C., Grove, J., Bey, P., Anti‐proliferative properties of DL‐α‐difluoromethyl ornithiae in cultured cells: A consequence of the irreversible inhibition of ornithine decarboxylase (1978) Biochem. Biophys. Res. Commun., 81, pp. 58-66 
504 |a Medrano, E.E., Pardee, A.B., Prevalent deficiency in tumor cells of cycloheximide‐induced cycle arrest (1980) Proc. Natl. Acad. Sci. U.S.A., 77, pp. 4123-4126 
504 |a Miller, E.C., Miller, J.A., Biochemistry of carcinogenesis (1959) Annu. Rev. Biochem., 28, pp. 291-320 
504 |a Pardee, A.B., A restriction point for control of normal animal cell proliferation (1974) Proc. Natl. Acad. Sci. U.S.A., 71, pp. 1286-1290 
504 |a Pardee, A.B., James, L.J., Selective killing of transformed baby hamster kidney (BHK) cells (1975) Proc. Natl. Acad. Sci. U.S.A., 72, pp. 4994-4998 
504 |a Pardee, A.B., Dubrow, R., Hamlin, J., Kletzien, R.F., Animal cell cycle (1978) Annu. Rev. Biochem., 47, pp. 715-750 
504 |a Rupniak, H.T., Paul, D., Regulation of the cell cycle by polyamines in normal and transformed fibroblasts (1978) Advances in Polyamine Research, 1, pp. 117-126. , R. A. Campbell, D. R. Morris, D. Bartos, G. Doyle, Daves, and, F. Bartos, Raven Press, New York 
504 |a Schneider, E.L., Stanbridge, E.J., Epstein, C.J., Incorporation of [3H]‐uridine and [3H]‐uracil into RNA: A simple technique for the detection of mycoplasma contamination of cultured cells (1974) Exp. Cell Res., 84, pp. 311-318 
504 |a Stoscheck, C.M., Erwin, B.G., Florini, J.R., Richman, R.A., Pegg, A.E., Effects of inhibitors of ornithine and S‐adenosylmethionine decarboxylases on L6 myoblast proliferation (1982) J Cell. Physiol., 110, pp. 161-168 
504 |a Sunkara, P.S., Pargac, M.B., Nishioka, K., Rao, P.N., Differential effects of inhibition of polyamine biosynthesis on cell cycle traverse and structure of the prematurely condensed chromosomes of normal and transformed cells (1979) J. Cell. Physiol., 98, pp. 451-458 
520 3 |a In order to study the effect of polyamine depletion on growth and proliferation of untransformed and chemically transformed cells, α‐difluoromethyl‐ornithine (DFMO) was added to cultures of 3T3 cells and their benzo[a]pyrene derivative BP‐3T3. Both types of cells stopped their proliferation after 72 hr of treatment with the inhibitor. When DFMO was removed and cells were cultivated afterwards in fresh medium without the drug, untransformed cells resumed growth after a lag period, whereas transformed cells were unable to proliferate unless exogenous polyamine was added. These alterations showed a strict correlation with intracellular polyamine pools, since after removal of DFMO from the culture medium, polyamine concentrations increased to almost normal values in 3T3 cells, but remained at low levels or decreased even more in the transformed cells BP‐3T3. The analysis by sodium dodecyl sulfate‐polyacrylamide gel electrophoresis of labeled proteins of both cell extracts has indicated that the described control of cell proliferation by intracellular polyamine levels might be related to the synthesis of at least two proteins with molecular weights of about 36,000 and 55,000 daltons. Copyright © 1983 Wiley‐Liss, Inc.  |l eng 
593 |a Instituto de Investigaciones Bioquimicas "Fundacion Campomar" and Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Obligado 2490, Buenos Aires, 1428, Argentina 
690 1 0 |a BENZO[A]PYRENE 
690 1 0 |a EFLORNITHINE 
690 1 0 |a POLYAMINE 
690 1 0 |a PUTRESCINE 
690 1 0 |a RADIOISOTOPE 
690 1 0 |a SPERMIDINE 
690 1 0 |a ANIMAL CELL 
690 1 0 |a CANCER CHEMOTHERAPY 
690 1 0 |a CELL CULTURE 
690 1 0 |a CELL PROLIFERATION 
690 1 0 |a CELL TRANSFORMATION 
690 1 0 |a DRUG THERAPY 
690 1 0 |a IN VITRO STUDY 
690 1 0 |a MOUSE 
690 1 0 |a NONHUMAN 
690 1 0 |a RETICULOENDOTHELIAL SYSTEM 
690 1 0 |a THERAPY 
690 1 0 |a THYMIDINE H 3 
690 1 0 |a ANIMAL 
690 1 0 |a BENZO(A)PYRENE 
690 1 0 |a BENZOPYRENES 
690 1 0 |a CARCINOGENS 
690 1 0 |a CELL DIVISION 
690 1 0 |a CELL TRANSFORMATION, NEOPLASTIC 
690 1 0 |a CELLS, CULTURED 
690 1 0 |a DNA REPLICATION 
690 1 0 |a EFLORNITHINE 
690 1 0 |a INTERPHASE 
690 1 0 |a KINETICS 
690 1 0 |a MICE 
690 1 0 |a MICE, INBRED BALB C 
690 1 0 |a ORNITHINE 
690 1 0 |a ORNITHINE DECARBOXYLASE 
690 1 0 |a PUTRESCINE 
690 1 0 |a SUPPORT, NON-U.S. GOV'T 
690 1 0 |a TRANSCRIPTION, GENETIC 
690 1 0 |a TRANSLATION, GENETIC 
700 1 |a Goldemberg, S.H. 
700 1 |a Algranati, I.D. 
773 0 |d 1983  |g v. 117  |h pp. 141-147  |k n. 2  |p J. Cell. Physiol.  |x 00219541  |w (AR-BaUEN)CENRE-2700  |t Journal of Cellular Physiology 
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856 4 0 |u https://doi.org/10.1002/jcp.1041170202  |y DOI 
856 4 0 |u https://hdl.handle.net/20.500.12110/paper_00219541_v117_n2_p141_Medrano  |y Handle 
856 4 0 |u https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00219541_v117_n2_p141_Medrano  |y Registro en la Biblioteca Digital 
961 |a paper_00219541_v117_n2_p141_Medrano  |b paper  |c PE 
962 |a info:eu-repo/semantics/article  |a info:ar-repo/semantics/artículo  |b info:eu-repo/semantics/publishedVersion 
999 |c 73122 

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