Initiation of glycogen biosynthesis in Escherichia coli studies of the properties of the enzymes involved
The properties of the enzymes involved in the initiation of glycogen biosynthesis in Escherichia coli were studied. It was found that the enzymic activities which transfer the glycosyl residues from UDPglucose or ADPglucose for the glucoprotein synthesis had differing stabilities upon storage at 4°C...
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| Formato: | Capítulo de libro |
| Lenguaje: | Inglés |
| Publicado: |
1978
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| Acceso en línea: | Registro en Scopus DOI Handle Registro en la Biblioteca Digital |
| Aporte de: | Registro referencial: Solicitar el recurso aquí |
| LEADER | 05431caa a22006617a 4500 | ||
|---|---|---|---|
| 001 | PAPER-12286 | ||
| 003 | AR-BaUEN | ||
| 005 | 20230518204229.0 | ||
| 008 | 190411s1978 xx ||||fo|||| 00| 0 eng|d | ||
| 024 | 7 | |2 scopus |a 2-s2.0-0018181202 | |
| 024 | 7 | |2 cas |a glycogen, 9005-79-2; Adenosine Diphosphate Glucose, 2140-58-1; Glucosyltransferases, EC 2.4.1.-; Glycogen Synthase, EC 2.4.1.11; Glycogen, 9005-79-2; Uridine Diphosphate Glucose, 133-89-1 | |
| 040 | |a Scopus |b spa |c AR-BaUEN |d AR-BaUEN | ||
| 030 | |a BBGSB | ||
| 100 | 1 | |a Barengo, R. | |
| 245 | 1 | 0 | |a Initiation of glycogen biosynthesis in Escherichia coli studies of the properties of the enzymes involved |
| 260 | |c 1978 | ||
| 270 | 1 | 0 | |m Barengo, R.; Instituto de Investigaciones Bioquimicas Fundación Campomar, Facultad de Ciencias Exactas y Naturales, Obligado 2490, 1428 Buenos Aires, Argentina |
| 506 | |2 openaire |e Política editorial | ||
| 504 | |a Krisman, (1972) Biochem. Biophys. Res. Commun., 46, pp. 1202-1212 | ||
| 504 | |a Krisman, Barengo, (1975) Eur. J. Biochem., 52, pp. 117-123 | ||
| 504 | |a Butler, Lee, Whelan, (1977) Carbohydr. Res., 55, pp. 73-82 | ||
| 504 | |a Barengo, Flawiá, Krisman, (1975) FEBS Lett., 53, pp. 274-278 | ||
| 504 | |a Krebs, Preiss, (1975) MTP International Review of Sciences — Biochemistry of Carbohydrates — Biochemistry Series One, 5, pp. 337-389. , 5th edn., W.J. Whelan, Butterworths-University Park Press, London | ||
| 504 | |a Wright, Robbins, (1965) Biochim. Biophys. Acta, 104, pp. 594-596 | ||
| 504 | |a Recondo, Dankert, Leloir, Isolation of adenosine diphosphate D-glucose from corn graiws (1965) Biochemical and Biophysical Research Communications, 12, pp. 204-207 | ||
| 504 | |a Lowry, Rosebrough, Farr, Randall, (1961) J. Biol. Chem., 193, pp. 265-275 | ||
| 504 | |a Krisman, (1962) Anal. Biochem., 4, pp. 17-23 | ||
| 504 | |a Barengo, Krisman, (1976) Acta Physiol. Latinoamer., 26, pp. 289-296 | ||
| 504 | |a Lavintman, Tandecarz, Carceller, Mendiara, Cardini, (1974) Eur. J. Biochem., 50, pp. 145-155 | ||
| 504 | |a Fontana, Krisman, (1978) Biochim. Biophys. Acta, 540, pp. 183-189 | ||
| 504 | |a Ward, Wright, Cell-Wall Synthesis in Dictyostelium discoideum. I. In Vitro Synthesis from Uridine Diphosphoglucose* (1965) Biochemistry, 4, pp. 2021-2026 | ||
| 504 | |a Gunja-Smith, Patil, Smith, (1977) J. Bacteriol., 130, pp. 818-825 | ||
| 520 | 3 | |a The properties of the enzymes involved in the initiation of glycogen biosynthesis in Escherichia coli were studied. It was found that the enzymic activities which transfer the glycosyl residues from UDPglucose or ADPglucose for the glucoprotein synthesis had differing stabilities upon storage at 4°C. The small amount of glycogen and the saccharide firmly bound to the membrane preparation, were degraded during the storage period. The activity measured in fresh and in stored preparations gave different time dependence curves. The stored preparation had a lag period which could be due to the transfer of the first glucose units to the protein. Both UDPglucose and ADPglucose: protein glucosyltransferases were affected in different ways by detergents. Based on the results presented, it may be concluded that both enzymatic activities are due to different enzymes. Furthermore, both enzymatic activities are different from that which transfers glucose from ADPglucose to glycogen. The following mechanism for the de novo synthesis is suggested. Glycogen in E. coli could be initiated by two different enzymes which transfer glucose to a protein acceptor either from UDPglucose or ADPglucose. Once the saccharide linked to the protein has reached a certain size it is almost exclusively enlarged by another ADPglucose-dependent enzyme. The participation of branching enzyme will produce a polysaccharide with the characteristics of glycogen. © 1978. |l eng | |
| 593 | |a Instituto de Investigaciones Bioquimicas Fundación Campomar, Facultad de Ciencias Exactas y Naturales, Obligado 2490, 1428 Buenos Aires, Argentina | ||
| 690 | 1 | 0 | |a GLYCOGEN |
| 690 | 1 | 0 | |a ADENOSINE DIPHOSPHATE GLUCOSE:PROTEIN GLUCOSYLTRANSFERASE |
| 690 | 1 | 0 | |a ANIMAL EXPERIMENT |
| 690 | 1 | 0 | |a ESCHERICHIA COLI |
| 690 | 1 | 0 | |a IN VITRO STUDY |
| 690 | 1 | 0 | |a URIDINE DIPHOSPHATE GLUCOSE:PROTEIN GLUCOSYLTRANSFERASE |
| 690 | 1 | 0 | |a ADENOSINE DIPHOSPHATE GLUCOSE |
| 690 | 1 | 0 | |a ESCHERICHIA COLI |
| 690 | 1 | 0 | |a GLUCOSYLTRANSFERASES |
| 690 | 1 | 0 | |a GLYCOGEN |
| 690 | 1 | 0 | |a GLYCOGEN SYNTHASE |
| 690 | 1 | 0 | |a KINETICS |
| 690 | 1 | 0 | |a SUBSTRATE SPECIFICITY |
| 690 | 1 | 0 | |a URIDINE DIPHOSPHATE GLUCOSE |
| 700 | 1 | |a Krisman, C.R. | |
| 773 | 0 | |d 1978 |g v. 540 |h pp. 190-196 |k n. 2 |p Biochim. Biophys. Acta Gen. Subj. |x 03044165 |w (AR-BaUEN)CENRE-2260 |t BBA - General Subjects | |
| 856 | 4 | 1 | |u https://www.scopus.com/inward/record.uri?eid=2-s2.0-0018181202&doi=10.1016%2f0304-4165%2878%2990131-9&partnerID=40&md5=0f5161422dba6952de973ceebbd4c21c |y Registro en Scopus |
| 856 | 4 | 0 | |u https://doi.org/10.1016/0304-4165(78)90131-9 |y DOI |
| 856 | 4 | 0 | |u https://hdl.handle.net/20.500.12110/paper_03044165_v540_n2_p190_Barengo |y Handle |
| 856 | 4 | 0 | |u https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03044165_v540_n2_p190_Barengo |y Registro en la Biblioteca Digital |
| 961 | |a paper_03044165_v540_n2_p190_Barengo |b paper |c PE | ||
| 962 | |a info:eu-repo/semantics/article |a info:ar-repo/semantics/artículo |b info:eu-repo/semantics/publishedVersion | ||
| 963 | |a VARI | ||
| 999 | |c 73239 | ||