Resistance in cholinesterase activity after an acute and subchronic exposure to azinphos-methyl in the freshwater gastropod Biomphalaria straminea
Organophosphorous and carbamates insecticides are ones of the most popular classes of pesticides used in agriculture. Its success relies on their high acute toxicity and rapid environmental degradation. These insecticides inhibit cholinesterase and cause severe effects on aquatic non-target species,...
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Academic Press
2014
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| LEADER | 24976caa a22023057a 4500 | ||
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| 001 | PAPER-14389 | ||
| 003 | AR-BaUEN | ||
| 005 | 20230518204454.0 | ||
| 008 | 190411s2014 xx ||||fo|||| 00| 0 eng|d | ||
| 024 | 7 | |2 scopus |a 2-s2.0-84906761384 | |
| 024 | 7 | |2 cas |a 1,5 bis(4 allyldimethylammoniumphenyl)pentan 3 one dibromide, 402-40-4; acetylthiocholine, 1797-69-9, 4468-05-7; azinphos methyl, 86-50-0; butyrylthiocholine, 1866-16-6, 4555-00-4; carboxylesterase, 59536-71-9, 83380-83-0, 9016-18-6, 9028-01-7; cholinesterase, 9001-08-5; iso OMPA, 513-00-8; physostigmine, 57-47-6, 64-47-1; Azinphosmethyl; Biological Markers; Carboxylic Ester Hydrolases; Cholinesterases; Insecticides; Water Pollutants, Chemical | |
| 040 | |a Scopus |b spa |c AR-BaUEN |d AR-BaUEN | ||
| 030 | |a EESAD | ||
| 100 | 1 | |a Bianco, K. | |
| 245 | 1 | 0 | |a Resistance in cholinesterase activity after an acute and subchronic exposure to azinphos-methyl in the freshwater gastropod Biomphalaria straminea |
| 260 | |b Academic Press |c 2014 | ||
| 270 | 1 | 0 | |m Kristoff, G.; IQUIBICEN-CONICET, Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Pab. II. 4to piso, 1428 Buenos Aires, Argentina; email: gkristoff@qb.fcen.uba.ar |
| 506 | |2 openaire |e Política editorial | ||
| 504 | |a Aldridge, W.N., Two types of esterases (A and B) hydrolysing p-nitrophenyl acetate, propionate and butyrate, and a method for their determination (1953) Biochem. J., 53, pp. 110-117 | ||
| 504 | |a Anguiano, O.L., Ferrari, A., Soleño, J., Martínez, M.C., Venturino, A., Pechen de D'Angelo, A.M., Montagna, C.M., Enhanced esterase activity and resistance to azinphosmethyl in target and nontarget organisms (2008) Environ. Toxicol. Chem., 27, pp. 2117-2123 | ||
| 504 | |a Anguiano, O.L., Catro, C., Venturino, A., Ferrari, A., (2012), http://dx.doi.org/10.1002/tox.21834,wileyonlinelibrary.com, Acute toxicity and biochemical effects of azinphos methyl in the amphipod Hyalella curvispina; Barata, C., Solayan, A., Porte, C., Role of B-esterases in assessing toxicity of organophosphorus (chlorpyrifos, malathion) and carbamate (carbofuran) pesticides to Daphnia magna (2004) Aquat. Toxicol., 66, pp. 125-139 | ||
| 504 | |a Basack, S.B., Oneto, M.L., Fuchs, J.S., Wood, E.J., Kesten, E.M., Esterases of Corbicula fluminea as biomarkers of exposure to organophosphorus pesticides (1998) Bull. Environ. Contam. Toxicol., 61, pp. 569-576 | ||
| 504 | |a Bianco, K., Yusseppone, M.S., Otero, S., Luquet, C., Ríos de Molina, M.C., Kristoff, G., Cholinesterases and neurotoxicity as highly sensitive biomarkers for an organophosphate insecticide in a freshwater gastropod (Chilina gibbosa) with low sensitivity carboxylesterases (2013) Aquat. Toxicol., pp. 26-35 | ||
| 504 | |a Bonacci, S., Browne, M.A., Dissanayake, A., Hagger, J.A., Corsi, I., Focardi, S., Galloway, T.S., Esterase activities in the bivalve mollusc Adamussium collecki as a biomarker for pollution monitoring in the Antarctic marine environment (2004) Mar. Pollut. Bull., 49, pp. 445-455 | ||
| 504 | |a Bonacci, S., Corsi, I., Focardi, S., Cholinesterases in the Antarctic scallop Adamussium colbecki: characterization and sensitivity to pollutants (2009) Ecotoxicol. Environ. Saf., 72, pp. 1481-1488 | ||
| 504 | |a Brown, M., Davies, I.M., Moffat, C.F., Redshaw, J., Craft, J.A., Characterisation of choline esterases and their tissue and subcellular distribution in mussel (Mytilus edulis) (2004) Mar. Environ. Res., 57, pp. 155-169 | ||
| 504 | |a Cacciatore, L.C., Kristoff, G., Verrengia Guerrero, N.R., Cochón, A.C., Binary mixtures of azinphos-methyl oxon and chlorpyrifos oxon produce in vitro synergistic cholinesterase inhibition in Planorbarius corneus (2012) Chemoshere, 84, pp. 450-458 | ||
| 504 | |a Cacciatore, L.C., Verrengia Guerrero, N.R., Cochón, A., Cholinesterase and carboxylesterase inhibition in Planorbarius corneus exposed to binary mixtures of azinphos-methyl and chlorpyrifos (2013) Aquat. Toxicol., pp. 124-134 | ||
| 504 | |a Carlock, L.L., Chen, W.L., Gordon, E.B., Killeen, J.C., Manley, A., Meyer, L.S., Regulating and assessing risks of cholinesterase-inhibiting pesticides: divergent approaches and interpretations (1999) J. Toxicol. Environ. Health B, 2, pp. 105-160 | ||
| 504 | |a Collange, B., Wheelock, C.E., Rault, M., Mazzia, C., Capowiez, Y., Sanchez-Hernandez, J.C., Inhibition, recovery and oxime-induced reactivation of muscle esterases following chlorpyrifos exposure in the earthworm Lumbricus terrestris (2010) Environ. Pollut., 158, pp. 2266-2272 | ||
| 504 | |a Di Salvatore, P., Calcagno, J.A., Ortíz, N., Ríos de Molina, M.C., Sabatini, S.E., Effect of seasonality on oxidative stress responses and metal accumulation in soft tissues of Aulacomya atra, a mussel from the South Atlantic Patagonian coast (2013) Mar. Environ. Res., 92, pp. 244-252 | ||
| 504 | |a Ellman, G.L., Courtney, K.D., Andres, V., Featherstone, R.M., A new and rapid colorimetric determination of acetylcholinesterase activity (1961) Biochem. Pharmacol., 7, pp. 88-95 | ||
| 504 | |a Escartín, E., Porte, C., The use of cholinesterase and carboxylesterase activities from Mytilus galloprovincialis in pollution monitoring (1997) Environ. Toxicol. Chem., 16, pp. 2090-2095 | ||
| 504 | |a Eto, M., (1974) Organophosphorous Pesticides: Organic and Biological Chemistry, , CRC Press, Ohio | ||
| 504 | |a Ferrari, A., Anguiano, O.L., Soleno, J., Venturino, A., Pechen de D'Angelo, A.M., Different susceptibility of two aquatic vertebrates (Oncorhynchus mykiss and Bufo arenarum) to azinphos methyl and carbaryl (2004) Comp. Biochem. Physiol., 139, pp. 239-243 | ||
| 504 | |a Ferrari, A., Lascano, C., Anguiano, O.L., Pechén de D'Angelo, A.M., Venturino, A., Antioxidant responses to azinphos methyl and carbaryl during the embriogenic development of the toad Rhinella (Bufo) arenarum Hensel (2009) Aquat. Toxicol., 93, pp. 37-44 | ||
| 504 | |a Ferrari, A., Venturino, A., Pechen de D'Angelo, A.M., Time course of brain cholinesterase inhibition and recovery following acute and subacute azinphos methyl, parathion and carbaryl exposure in the goldfish (Carassius auratus) (2004) Ecotoxicol. Environ. Saf., 57, pp. 420-425 | ||
| 504 | |a Ferrari, A., Venturino, A., Pechén de D'Angelo, A.M., Effects of carbaryl and azinphos methyl on juvenile rainbow trout (Oncorhynchus mykiss) detoxifying enzymes (2007) Pestic. Biochem. Physiol., 88, pp. 134-142 | ||
| 504 | |a Forget, J., Bocquené, G., Partial purification and enzymatic characterization of acetylchonisterase from the intertidal marine copepod Tigriopus brevicornis (1999) Comp. Biochem. Physiol., 123, pp. 345-350 | ||
| 504 | |a Fried, B., Sundar Rao, K., Sherma, J., Fatty acid composition of Biomphalaria glabrata (Gastropoda: Planorbidae) fed hens[U+05F3] egg yolk versus leaf lettuce (1992) Comp. Biochem. Physiol., 101, pp. 351-352 | ||
| 504 | |a Fulton, M.H., Key, P.B., Acetylcholinesterase inhibition in estuarine fish and invertebrates as an indicator of organophosphorus insecticide exposure and effects (2001) Environ. Toxicol. Chem., 20, pp. 37-45 | ||
| 504 | |a Gagnaire, B., Geffard, O., Xuereb, B., Margoum, C., Garric, J., Cholinesterase activities as potential biomarkers: characterzation in two freshwater snails, Potamopyrgus antipodarum (Mollusca, Hydrobiidae, Smith 1889) and Valvata piscinalis (Mollusca, Valvatidae, Muller 1774) (2008) Chemosphere, 71, pp. 553-560 | ||
| 504 | |a Galloway, T.S., Millward, N., Browne, M.A., Depledge, M.H., Rapid assessment of organophosphorus / carbamate exposure in the bivalve mollusk Mytilus edulis using combined esterase activities as biomarkers (2002) Toxicology, 61, pp. 169-180 | ||
| 504 | |a González, C.O., Fernández de la Puente, G., Quiroga, A.M., Franco, H.R., Semenszczuk, N., Bravin, C., Valoración de colinesterasas en coloros tabacaleros (2006) Acta Toxicol. Argent., 14, p. 20 | ||
| 504 | |a González Vejares, S., Sabat, P., Sanchez-Hernandez, J.C., Tissue-specific inhibition and recovery of esterase activities in Lumbriculs terrestres experimentally exposed to chlorpyrifos (2010) Comp. Biochem. Physiol., 151, pp. 351-359 | ||
| 504 | |a Granovsky, A.V., Ma, L., Ricaud, R., Bengtson, R.L., Selim, H.M., Fate of azinphosmethyl in a sugarcane field: distributions in canopy, soil, and runoff (1996) J. Environ. Qual., 25, pp. 1210-1216 | ||
| 504 | |a Hannam, M.L., Hagger, J.A., Jones, M.B., Galloway, T.S., Characterisation of esterases as potential biomarkers of pesticide exposure in the lugworm Arenicola marina (Annelida: Polychaeta) (2008) Environ. Pollut., 152, pp. 342-350 | ||
| 504 | |a Jokanovic, M., Biotransformation of organophosphorus compounds (2001) Toxicology, 166, pp. 139-160 | ||
| 504 | |a Klosterhaus, S.L., Di Pinto, L.M., Chandler, G.T., A comparative assessment of azinphosmethyl bioaccumulation and toxicity in two estuarine meiobenthic harpacticoid copepods (2003) Environ. Toxicol. Chem., 22, pp. 2960-2968 | ||
| 504 | |a Kristoff, G., Cacciatore, L.C., Verrengia Guerrero, N.R., Cochón, A.C., Effects of the organophosphate insecticide azinphos-methyl on the reproduction and cholinesterase activity of Biomphalaria glabrata (2011) Chemosphere, 84, pp. 585-591 | ||
| 504 | |a Kristoff, G., Chiny Barrionuevo, D., Cacciatore, L.C., Verrengia Guerrero, N.R., Cochón, A., In vivo studies on inhibition and recuperation of B-esterase activity in Biomphalaria glabrata exposed to azinphos-methyl: analysis of enzyme, substrate and tissue dependence (2012) Aquat. Toxicol., pp. 19-26 | ||
| 504 | |a Kristoff, G., Verrengia Guerrero, N., Cochón, A., Effects of azinphos-methyl exposure on enzymatic and non-enzymatic antioxidant defenses in Biomphalaria glabrata and Lumbriculus variegatus (2008) Chemosphere, 72, pp. 1333-1339 | ||
| 504 | |a Kristoff, G., Verrengia Guerrero, N.R., Cochón, A.C., Inhibition of cholinesterases and carboxylesterases of two invertebrate species, Biomphalaria glabrata and Lumbriculus variegatus, by the carbamate pesticide carbaryl (2010) Aquat. Toxicol., 96, pp. 115-123 | ||
| 504 | |a Kristoff, G., Verrengia Guerrero, N.R., Pechén de D'Angelo, A.M., Cochón, A.C., Inhibition of cholinesterase activity by azinphos-methyl in two freshwater invertebrates: Biomphalaria glabrata and Lumbriculus variegatus (2006) Toxicology, 222, pp. 185-194 | ||
| 504 | |a Korochkin, L.I., Genetic regulation of isozyme patterns in Drosophila during development (1980) Isozymes: Current Topics in Medical and Biological Research, 304, pp. 159-202. , Alan R. Liss, New York | ||
| 504 | |a Kubli, E., The sex peptide (1992) BioEssays, 14, pp. 779-784 | ||
| 504 | |a Laguerre, C., Sánchez-Hernández, J., Köhler, H.R., Triebskorn, R., Capowiez, Y., Rault, M., Mazzia, C., B-type esterases in the snail Xeropicta derbentina: an enzymological analysis to evaluate their use as biomarkers of pesticide exposure (2009) Environ. Pollut., 157, pp. 199-207 | ||
| 504 | |a Loewy, M., Kirs, V., Carbajal, G., Venturino, A., Pechén de D'Angelo, A.M., Groundwater contamination by azinphos-methyl in the Northern Patagonic Region (Argentina) (1999) Sci. Total Environ., 225, pp. 211-218 | ||
| 504 | |a Loewy, R.M., Monza, L.B., Kiers, V.E., Savini, M.C., Pesticide distribution in an agricultural environment in Argentina (2011) J. Environ. Sci. Health B, 46, pp. 662-670 | ||
| 504 | |a Lowry, O.H., Rosebrough, N.J., Farr, A.L., Randall, R.J., Protein measurement with the folin phenol reagent (1951) J. Biol. Chem., 193, pp. 265-275 | ||
| 504 | |a Massoulié, J., Pezzementi, L., Bon, S., Krejci, E., Vallette, F.M., Molecular and cellular biology of cholinesterases (1993) Prog. Neurobiol., 41, pp. 31-91 | ||
| 504 | |a Mikhailov, A.I., Torrado, M., Korochkin, L.I., Kopantzeva, M.A., Méndez, J., Male predominant carboxylesterase expression in the reproductive system of molluscs and insects: immunochemical and biochemical similarity between Mytilus male associated polypeptide (MAP) and Drosophila sex-specific esterase (1997) Comp. Biochem. Physiol., 118, pp. 197-208 | ||
| 504 | |a Mikhailov, A.T., Torrado, M., Carboxylesterase overexpression in the mail reproductive tract: a universal safeguarding mechanism? (1999) Reprod. Fertil. Dev., 11, pp. 133-145 | ||
| 504 | |a Mora, P., Fournier, D., Narbonne, J.F., Cholinesterases from the marine mussels Mytilus galloprovincialis L. and M. Edulis L. and from the freshwater bivalve Corbicula fluminea Muller (1999) Comp. Biochem. Physiol., 122, pp. 353-361 | ||
| 504 | |a , pp. 3-9. , NRC: Committee on Biological Markers of the National Research Council, 1987. Biological markers in environmental health research. Environ. Health Perspect. 74; Öruc, O.E., Sevgiler, Y., Üner, N., Tissue-specific oxidative stress responses in fish exposed to 2,4-D and azinphosmethyl (2004) Comp. Biochem. Physiol., 137, pp. 43-51 | ||
| 504 | |a Peña-Llopis, S., Ferrando, M.D., Peña, J.B., Impaired glutathione redox status is associated with decreased survival in two organophosphate-poisoned marine bivalves (2002) Chemosphere, 47, pp. 485-497 | ||
| 504 | |a Peña-Llopis, S., Ferrando, M.D., Peña, J.B., Fish tolerance to organophosphate-induced oxidative stress is dependent on the glutathione metabolism and enhanced by N-acetylcysteine (2003) Aquat. Toxicol., 65, pp. 337-360 | ||
| 504 | |a Rivadeneira, P.R., Agrelo, M., Otero, S., Kristoff, G., Different effects of subchronic exposure to low concentrations of the organophosphate insecticide chlorpyrifos in a freshwater gastropod (2013) Ecotoxicol. Environ. Saf., 90, pp. 82-88 | ||
| 504 | |a Rumi, A., Gutierrez Gregoric, D.E., Núñez, V., Darrigan, G.A., Malacología Latinoamericana. Moluscos de agua dulce de Argentina (2008) Rev. Biol. Trop., 56, pp. 77-111 | ||
| 504 | |a Sanchez-Hernandez, J.C., Ecotoxicological perspectives of B-esterases in the assessment of pesticide contamination (2007) Environmental Pollution: New Research, pp. 1-45. , Nova Science Publishers, Inc., N.Y., USA, R.H. Plattenberg (Ed.) | ||
| 504 | |a Sanchez-Hernandez, J.C., Wheelock, C.E., Tissue distribution, isozyme abundance and sensitivity to chorpyrifos-oxon of carboxylesterases in the earthworm Lumbricus terrestris (2009) Environ. Pollut., 157, pp. 264-272 | ||
| 504 | |a Schulz, R., Field studies on exposure, effects, and risk mitigation of aquatic nonpoint-source insecticide pollution: a review (2004) J. Environ. Qual., 33, pp. 419-448 | ||
| 504 | |a Talesa, V., Contenti, S., Mangiabene, C., Pascolini, R., Rosi, G., Principato, G.B., Propionylcholinesterase from Murex brandaris: comparison with other invertebrate cholinesterases (1990) Comp. Biochem. Physiol., 96, pp. 39-43 | ||
| 504 | |a Tripathi, P.K., Singh, A., Carbaryl induced alterations in the reproduction and metabolism of the freshwater snail Lymnaea acuminata (2004) Pest. Biochem. Physiol., 79, pp. 1-9 | ||
| 504 | |a Varó, I., Navarro, J.C., Amat, F., Guilhermino, L., Characterization of cholinesterases and evaluation of the inhibitory potential of chlorpyrifos and dichlorvos to Artemia salina and Artemia parthenogenetica (2002) Chemosphere, 48, pp. 563-569 | ||
| 504 | |a Venturino, A., Anguiano, O.L., Gauna, L., Cocca, C., Bergoc, R.M., Pechén de D'Angelo, A.M., Thiols and polyamines in the potentiation of malathion toxicity in larval stages of the toad Bufo arenarum (2001) Comp. Biochem. Physiol., 130, pp. 191-198 | ||
| 504 | |a Vioque-Fernández, A., Alves de Almeida, E., López-Barea, J., Esterases as pesticide biomarkers in crayfish (Procambarus clarkii, Crustacea): tissue distribution, sensitivity to model compounds and recovery from inactivation (2007) Comp. Biochem. Physiol., 145, pp. 404-412 | ||
| 504 | |a Xuereb, B., Noury, P., Felten, V., Garric, J., Geffard, O., Cholinesterase activity in Gammarus pulex (Crustacea Amphipoda): characterization and effects of chlorpyrifos (2007) Toxicology, 236, pp. 178-189 | ||
| 504 | |a Xuereb, B., Chaumot, A., Mons, R., Garric, J., Geffard, O., Acetylcholinesterase activity in Gammarus fossarum (Crustacea Amphipoda). Intrinsic variability, reference levels, and a reliable tool for field surveys (2009) Aquat. Toxicol., 93, pp. 225-233 | ||
| 504 | |a Wan, M.T., Szeto, S.Y., Price, P., Distribution and persistence of azinphos-methyl and parathion in chemigated cranberry bogs (1995) J. Environ. Qual., 24, pp. 589-596 | ||
| 504 | |a Wheelock, C.E., Phillips, B.M., Anderson, B.S., Miller, M.J., Hammock, B.D., Applications of carboxylesterase activity in environmental monitoring and toxicity identification evaluations (TIEs) (2008) Rev. Environ. Contam. Toxicol., 195, pp. 117-178 | ||
| 504 | |a Winston, G.W., Di Giulio, R.T., Prooxidant and antioxidant mechanisms in aquatic organisms (1991) Aquat. Toxicol., 19, pp. 137-161 | ||
| 504 | |a Yang, B., Yang, D., Brady, M., Parkinson, A., Rat testicular carboxylesterase: cloning, cellular localization, and relationship to liver hydrolases A (1995) Arch. Biochem. Biophys., 316, pp. 899-908 | ||
| 520 | 3 | |a Organophosphorous and carbamates insecticides are ones of the most popular classes of pesticides used in agriculture. Its success relies on their high acute toxicity and rapid environmental degradation. These insecticides inhibit cholinesterase and cause severe effects on aquatic non-target species, particularly in invertebrates. Since the properties of cholinesterases may differ between species, it is necessary to characterize them before their use as biomarkers. Also organophosphorous and carbamates inhibit carboxylesterases and the use of both enzymes for biomonitoring is suggested. Azinphos-methyl is an organophosphorous insecticide used in several parts of the word. In Argentina, it is the most applied insecticide in fruit production in the north Patagonian region. It was detected with the highest frequency in superficial and groundwater of the region. This work aims to evaluate the sensitivity of B. straminea cholinesterases and carboxylesterases to the OP azinphos-methyl including estimations of 48h NOEC and IC50 of the pesticide and subchronic effects at environmentally relevant concentrations. These will allow us to evaluate the possibility of using cholinesterase and carboxylesterase of B. straminea as sensitive biomarkers. Previously a partial characterization of these enzymes will be performed. As in most invertebrates, acetylthiocholine was the preferred hydrolyzed substrate of B. straminea ChE, followed by propionylthiocholine and being butyrylthiocholine hydrolysis very low. Cholinesterase activity of B. straminea was significantly inhibited by the selective cholinesterases inhibitor (eserine) and by the selective inhibitor of mammalian acethylcholinesterase (BW284c51). In contrast, iso-OMPA, a specific inhibitor of butyrylcholinesterase, did not inhibit cholinesterase activity. These results suggest that cholinesterase activity in total soft tissue of B. straminea corresponds to acethylcholinesterase. Carboxylesterases activity was one order of magnitude higher than cholinesterase. A greater efficiency (Vmax/Km) was obtained using acetylthiocholine and p-nitrophenyl butyrate. Acute exposure to azinphos-methyl did not cause inhibition of cholinesterase activity until 10mgL-1 used. Carboxylesterases towards p-nitrophenyl butyrate was inhibited by azinphos-methyl being the IC502.20±0.75mgL-1 of azinphos-methyl. Subchronic exposure to environmental concentrations of azinphos-methyl (0.02 and 0.2mgL-1) produced a decrease in survival, protein content and carboxylesterases activity despite no inhibition of cholinesterase activity was observed. B. straminea cholinesterase is not a sensible biomarker. On the contrary, carboxylesterases activity was inhibited by azinphos-methyl. Carboxylesterases could be protecting cholinesterase activity and therefore, protecting the organism from neurotoxicity. This work confirms the advantages of measuring cholinesterases and carboxylesterases jointly in aquatic biomonitoring of pesticide contamination. This becomes relevant in order to find more sensitive biomarkers and new strategies to protect non-target aquatic organisms from pesticide contamination. © 2014 Elsevier Inc. |l eng | |
| 536 | |a Detalles de la financiación: Universidad de Buenos Aires, 20020110100070 | ||
| 536 | |a Detalles de la financiación: Consejo Nacional de Investigaciones Científicas y Técnicas, 11220120100680, 4541-12 | ||
| 536 | |a Detalles de la financiación: This work was supported by grants from CONICET , Argentina ( Resolution 4541-12 and 11220120100680 CO ) and Universidad de Buenos Aires, Argentina ( 20020110100070 ). We thank Dra. M. Ostrowsky. | ||
| 593 | |a Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Argentina | ||
| 593 | |a Departamento de Biodiversidad y Biología Experimental, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Argentina y CONICET, Argentina | ||
| 593 | |a IQUIBICEN-CONICET, Argentina | ||
| 690 | 1 | 0 | |a BIOMARKERS |
| 690 | 1 | 0 | |a CARBOXYLESTERASES |
| 690 | 1 | 0 | |a CHOLINESTERASES |
| 690 | 1 | 0 | |a ORGANOPHOSPHOROUS INSECTICIDES |
| 690 | 1 | 0 | |a PROTEINS |
| 690 | 1 | 0 | |a 1,5 BIS(4 ALLYLDIMETHYLAMMONIUMPHENYL)PENTAN 3 ONE DIBROMIDE |
| 690 | 1 | 0 | |a ACETYLTHIOCHOLINE |
| 690 | 1 | 0 | |a AZINPHOS METHYL |
| 690 | 1 | 0 | |a BUTYRYLTHIOCHOLINE |
| 690 | 1 | 0 | |a CARBOXYLESTERASE |
| 690 | 1 | 0 | |a CHOLINE DERIVATIVE |
| 690 | 1 | 0 | |a CHOLINESTERASE |
| 690 | 1 | 0 | |a CHOLINESTERASE INHIBITOR |
| 690 | 1 | 0 | |a ISO OMPA |
| 690 | 1 | 0 | |a PHYSOSTIGMINE |
| 690 | 1 | 0 | |a PROPIONYLTHIOCHOLINE |
| 690 | 1 | 0 | |a UNCLASSIFIED DRUG |
| 690 | 1 | 0 | |a AZINPHOS METHYL |
| 690 | 1 | 0 | |a BIOLOGICAL MARKER |
| 690 | 1 | 0 | |a CARBOXYLESTERASE |
| 690 | 1 | 0 | |a CHOLINESTERASE |
| 690 | 1 | 0 | |a INSECTICIDE |
| 690 | 1 | 0 | |a WATER POLLUTANT |
| 690 | 1 | 0 | |a ACETYLTHIOCHOLINE |
| 690 | 1 | 0 | |a BIOLOGICAL MARKER |
| 690 | 1 | 0 | |a BUTYRYLTHIOCHOLINE |
| 690 | 1 | 0 | |a CHOLINE DERIVATIVE |
| 690 | 1 | 0 | |a FRESH WATER |
| 690 | 1 | 0 | |a PROPIONYLTHIOCHOLINE |
| 690 | 1 | 0 | |a AZINPHOS METHYL |
| 690 | 1 | 0 | |a INSECTICIDE |
| 690 | 1 | 0 | |a AZINPHOS METHYL |
| 690 | 1 | 0 | |a BIOMARKER |
| 690 | 1 | 0 | |a BIOMONITORING |
| 690 | 1 | 0 | |a CARBAMATE (ESTER) |
| 690 | 1 | 0 | |a CONCENTRATION (COMPOSITION) |
| 690 | 1 | 0 | |a ENZYME ACTIVITY |
| 690 | 1 | 0 | |a GASTROPOD |
| 690 | 1 | 0 | |a INSECTICIDE |
| 690 | 1 | 0 | |a PROTEIN |
| 690 | 1 | 0 | |a TOXICITY |
| 690 | 1 | 0 | |a NONTARGET ORGANISM |
| 690 | 1 | 0 | |a POLLUTION EXPOSURE |
| 690 | 1 | 0 | |a ANIMAL TISSUE |
| 690 | 1 | 0 | |a ARTICLE |
| 690 | 1 | 0 | |a BIOASSAY |
| 690 | 1 | 0 | |a BIOMPHALARIA STRAMINEA |
| 690 | 1 | 0 | |a CONCENTRATION (PARAMETERS) |
| 690 | 1 | 0 | |a CONTROLLED STUDY |
| 690 | 1 | 0 | |a ENZYME ACTIVITY |
| 690 | 1 | 0 | |a ENZYME INHIBITION |
| 690 | 1 | 0 | |a ENZYME KINETICS |
| 690 | 1 | 0 | |a EXPOSURE |
| 690 | 1 | 0 | |a HYDROLYSIS |
| 690 | 1 | 0 | |a IC 50 |
| 690 | 1 | 0 | |a IN VITRO STUDY |
| 690 | 1 | 0 | |a NONHUMAN |
| 690 | 1 | 0 | |a PROTEIN CONTENT |
| 690 | 1 | 0 | |a SENSITIVITY ANALYSIS |
| 690 | 1 | 0 | |a SURVIVAL |
| 690 | 1 | 0 | |a TISSUE HOMOGENATE |
| 690 | 1 | 0 | |a ANIMAL |
| 690 | 1 | 0 | |a BIOMPHALARIA |
| 690 | 1 | 0 | |a DRUG EFFECTS |
| 690 | 1 | 0 | |a ENVIRONMENTAL EXPOSURE |
| 690 | 1 | 0 | |a ENVIRONMENTAL MONITORING |
| 690 | 1 | 0 | |a ENZYMOLOGY |
| 690 | 1 | 0 | |a METABOLISM |
| 690 | 1 | 0 | |a TOXICITY |
| 690 | 1 | 0 | |a WATER POLLUTANT |
| 690 | 1 | 0 | |a ARTICLE |
| 690 | 1 | 0 | |a BIOLOGICAL MONITORING |
| 690 | 1 | 0 | |a IC50 |
| 690 | 1 | 0 | |a NEUROTOXICITY |
| 690 | 1 | 0 | |a PROTEIN ANALYSIS |
| 690 | 1 | 0 | |a PROTEIN FUNCTION |
| 690 | 1 | 0 | |a PROTEIN HYDROLYSIS |
| 690 | 1 | 0 | |a PROTEIN METABOLISM |
| 690 | 1 | 0 | |a PATAGONIA |
| 690 | 1 | 0 | |a ANIMALS |
| 690 | 1 | 0 | |a AZINPHOSMETHYL |
| 690 | 1 | 0 | |a BIOLOGICAL MARKERS |
| 690 | 1 | 0 | |a BIOMPHALARIA |
| 690 | 1 | 0 | |a CARBOXYLIC ESTER HYDROLASES |
| 690 | 1 | 0 | |a CHOLINESTERASES |
| 690 | 1 | 0 | |a ENVIRONMENTAL EXPOSURE |
| 690 | 1 | 0 | |a ENVIRONMENTAL MONITORING |
| 690 | 1 | 0 | |a INSECTICIDES |
| 690 | 1 | 0 | |a WATER POLLUTANTS, CHEMICAL |
| 690 | 1 | 0 | |a BIOMPHALARIA STRAMINEA |
| 690 | 1 | 0 | |a GASTROPODA |
| 651 | 4 | |a ARGENTINA | |
| 700 | 1 | |a Otero, S. | |
| 700 | 1 | |a Balazote Oliver, A. | |
| 700 | 1 | |a Nahabedian, D. | |
| 700 | 1 | |a Kristoff, G. | |
| 773 | 0 | |d Academic Press, 2014 |g v. 109 |h pp. 85-92 |p Ecotoxicol. Environ. Saf. |x 01476513 |w (AR-BaUEN)CENRE-4483 |t Ecotoxicology and Environmental Safety | |
| 856 | 4 | 1 | |u https://www.scopus.com/inward/record.uri?eid=2-s2.0-84906761384&doi=10.1016%2fj.ecoenv.2014.07.038&partnerID=40&md5=a920da3832004f6ff743dacf9740da92 |y Registro en Scopus |
| 856 | 4 | 0 | |u https://doi.org/10.1016/j.ecoenv.2014.07.038 |y DOI |
| 856 | 4 | 0 | |u https://hdl.handle.net/20.500.12110/paper_01476513_v109_n_p85_Bianco |y Handle |
| 856 | 4 | 0 | |u https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01476513_v109_n_p85_Bianco |y Registro en la Biblioteca Digital |
| 961 | |a paper_01476513_v109_n_p85_Bianco |b paper |c PE | ||
| 962 | |a info:eu-repo/semantics/article |a info:ar-repo/semantics/artículo |b info:eu-repo/semantics/publishedVersion | ||
| 963 | |a VARI | ||
| 999 | |c 75342 | ||