Transnitrosylation products of the dipeptide cysteinyl-cysteine: An examination by tandem mass spectrometry and density functional theory

The fragmentation pathways of protonated mono-and di-nitrosylated derivatives from the dipeptide Cys-Cys obtained by electrospray were examined. Protonated mononitrosylated dipeptide upon loss of NO formed a radical cation, which in turn shows two fragment ions, one from the loss of HS and the other...

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Autor principal: Butler, M.
Otros Autores: Michael Siu, K.W, Hopkinson, A.C
Formato: Capítulo de libro
Lenguaje:Inglés
Publicado: Royal Society of Chemistry 2016
Acceso en línea:Registro en Scopus
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024 7 |2 scopus  |a 2-s2.0-84958972650 
024 7 |2 cas  |a cysteine, 4371-52-2, 52-89-1, 52-90-4; Cysteine; cysteinylcysteine; Dipeptides 
040 |a Scopus  |b spa  |c AR-BaUEN  |d AR-BaUEN 
030 |a PPCPF 
100 1 |a Butler, M. 
245 1 0 |a Transnitrosylation products of the dipeptide cysteinyl-cysteine: An examination by tandem mass spectrometry and density functional theory 
260 |b Royal Society of Chemistry  |c 2016 
270 1 0 |m Butler, M.; Department of Chemistry and Centre for Research in Mass Spectrometry, York University, 4700 Keele Street, Canada; email: mbutler@qo.fcen.uba.ar 
506 |2 openaire  |e Política editorial 
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504 |a Osburn, S., Berden, G., Oomens, J., O'Hair, R.A.J., Ryzhov, V., (2011) J. Am. Soc. Mass Spectrom., 22, p. 1794 
504 |a Osburn, S., Burgie, T., Berden, G., Oomens, J., O'Hair, R.A.J., Ryzhov, V., (2013) J. Phys. Chem. A, 117, p. 1144 
504 |a Osburn, S., Berden, G., Oomens, J., Gulyuz, K., Polfer, N.C., O'Hair, R.A.J., Ryzhov, V., (2013) ChemPlusChem, 78, p. 970 
504 |a Zhao, J., Siu, K.W.M., Hopkinson, A.C., (2011) Org. Biomol. Chem., 9, p. 7384 
504 |a Osburn, S., Berden, G., Oomens, J., O'Hair, R.A.J., Ryzhov, V., (2012) J. Am. Soc. Mass Spectrom., 23, p. 1019 
504 |a Lam, A.K.Y., Ryzhov, V., O'Hair, R.A.J., (2010) J. Am. Soc. Mass Spectrom., 21, p. 1296 
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520 3 |a The fragmentation pathways of protonated mono-and di-nitrosylated derivatives from the dipeptide Cys-Cys obtained by electrospray were examined. Protonated mononitrosylated dipeptide upon loss of NO formed a radical cation, which in turn shows two fragment ions, one from the loss of HS and the other from a neutral loss giving a radical cation of formula C2H5NS+. Protonated dinitrosylated dipeptide dissociated by losing both NO molecules, forming a cyclic structure with a vicinal disulfide bridge whose major dissociation channel was the loss of CO. After CO loss, two pathways were observed (loss of NH3 and C2H3NS) which were preceded by proton exchange occurring between one β-carbon and the nitrogen atom. DFT calculations did not show significant differences in the energies involved for the loss of the NO radical from either of the cysteine residues of the protonated di-nitrosylated dipeptide. Upon loss of the first NO radical, the thiyl radical afforded the vicinal disulfide product with a small barrier through radical substitution of the remaining NO moiety. The calculated relative energy barriers for the different channels are in good agreement with experimental observations. Structures of the ions obtained after dissociation are suggested on the basis of the proposed mechanisms. © 2016 the Owner Societies.  |l eng 
593 |a Department of Chemistry and Centre for Research in Mass Spectrometry, York University, 4700 Keele Street, Toronto, ON M3J 1P3, Canada 
593 |a Department of Chemistry and Biochemistry, University of Windsor, 401 Sunset Avenue, Windsor, ON N9B 3P4, Canada 
593 |a Departamento de Quiimica Orgainica, UMyMFOR-CONICET, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Pabellón II, 31 piso, Buenos Aires, C1428EHA, Argentina 
690 1 0 |a CYSTEINE 
690 1 0 |a CYSTEINYLCYSTEINE 
690 1 0 |a DIPEPTIDE 
690 1 0 |a CHEMISTRY 
690 1 0 |a ELECTROSPRAY MASS SPECTROMETRY 
690 1 0 |a TANDEM MASS SPECTROMETRY 
690 1 0 |a THERMODYNAMICS 
690 1 0 |a CYSTEINE 
690 1 0 |a DIPEPTIDES 
690 1 0 |a SPECTROMETRY, MASS, ELECTROSPRAY IONIZATION 
690 1 0 |a TANDEM MASS SPECTROMETRY 
690 1 0 |a THERMODYNAMICS 
700 1 |a Michael Siu, K.W. 
700 1 |a Hopkinson, A.C. 
773 0 |d Royal Society of Chemistry, 2016  |g v. 18  |h pp. 6047-6052  |k n. 8  |p Phys. Chem. Chem. Phys.  |x 14639076  |t Physical Chemistry Chemical Physics 
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856 4 0 |u https://doi.org/10.1039/c5cp08014b  |y DOI 
856 4 0 |u https://hdl.handle.net/20.500.12110/paper_14639076_v18_n8_p6047_Butler  |y Handle 
856 4 0 |u https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_14639076_v18_n8_p6047_Butler  |y Registro en la Biblioteca Digital 
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