Tryglycerides in isolated rat uterine strips. Influences of glucose deprivation, 2-deoxy-D-glucose, estrogens and exogenous or endogenous prostaglandins E1, E2 and F2α
Triglyceride (TG) levels in uterine strips isolated from natural estrous, ovariectomized or ovariectomized, estradiol-injected rats, were explored. Determinations were performed either immediately after isolation (initial or 0 time) as well as after one hour period of incubation (60 min time) in glu...
Guardado en:
| Autor principal: | |
|---|---|
| Otros Autores: | , , , |
| Formato: | Capítulo de libro |
| Lenguaje: | Inglés |
| Publicado: |
1987
|
| Acceso en línea: | Registro en Scopus DOI Handle Registro en la Biblioteca Digital |
| Aporte de: | Registro referencial: Solicitar el recurso aquí |
| LEADER | 10534caa a22013217a 4500 | ||
|---|---|---|---|
| 001 | PAPER-18136 | ||
| 003 | AR-BaUEN | ||
| 005 | 20230518204930.0 | ||
| 008 | 190411s1987 xx ||||fo|||| 00| 0 eng|d | ||
| 024 | 7 | |2 scopus |a 2-s2.0-0023163849 | |
| 024 | 7 | |2 cas |a deoxyglucose, 154-17-6; estradiol, 50-28-2; glucose, 50-99-7, 84778-64-3; indometacin, 53-86-1, 74252-25-8, 7681-54-1; prostaglandin E1, 745-65-3; prostaglandin E2, 363-24-6; prostaglandin F2 alpha, 551-11-1; prostaglandin E, 11042-70-9; Alprostadil, 745-65-3; Deoxy Sugars; Deoxyglucose, 154-17-6; Dinoprost, 551-11-1; Dinoprostone, 363-24-6; Estradiol, 50-28-2; Estrogens; Glucose, 50-99-7; Indomethacin, 53-86-1; Prostaglandins E; Prostaglandins F; Prostaglandins; Triglycerides | |
| 040 | |a Scopus |b spa |c AR-BaUEN |d AR-BaUEN | ||
| 030 | |a PLMED | ||
| 100 | 1 | |a González, E.T. | |
| 245 | 1 | 0 | |a Tryglycerides in isolated rat uterine strips. Influences of glucose deprivation, 2-deoxy-D-glucose, estrogens and exogenous or endogenous prostaglandins E1, E2 and F2α |
| 260 | |c 1987 | ||
| 270 | 1 | 0 | |m González, E.T.; Centro de Estudios Farmacológicos y de Principios Naturales (CEFAPRIN), Consejo Nacional de Investigaciones Cientificas y Tecnicas de la Republica Argentina (CONICET). Serr, 1414 Buenos Aires, Argentina |
| 506 | |2 openaire |e Política editorial | ||
| 504 | |a Gonzalez, Torres, Gimeno, Gimeno, Substrate requirements in vitro for the spontaneous motility of uterine strips from ovariectomized or natural estrous rats (1976) Archives Of Physiology And Biochemistry, 84, p. 213 | ||
| 504 | |a Sterin, Linares, Goldraij, Gimeno, Gimeno, Prostaglandin E2 and Prostaglandin F2α regulation of triglyceride levels in uterine smooth muscle from restricted diet estrous and diestrous rats (1984) Prostagl. Lek. & Med., 14, p. 391 | ||
| 504 | |a Macciocchi, Linares, Goldraij, Gimeno, Gimeno, Different effects of prostaglandin E2 and F2α on triglyceride levels in uterine smooth muscle from diabetic diestrous rats (1985) Prostagl. Leuk. & Med., 20, p. 99 | ||
| 504 | |a Gimeno, Borda, Sterin-Borda, Vidal, Gimeno, Pharmacological influences on human ovarian contractions (1976) Obstet.Gynecol. (N.Y.), 47, p. 218 | ||
| 504 | |a Van Handel, Zilversmit, Micromethod for the direct determination of serum triglycerides (1957) J. Lab. Clin. Med., 50, p. 152 | ||
| 504 | |a Carlson, Determination of serum triglycerides (1963) J. Arther. Res., 3, p. 334 | ||
| 504 | |a McFadyen, Estimation of formaldehyde in biological mixtures (1945) J. Biol. Chem., 58, p. 107 | ||
| 504 | |a Webb, (1966) Enzyme and Metabolic Inhibitors, 3. , Academic Press, N.Y | ||
| 504 | |a Gimeno, Lacuara, Gimeno, Ceretti, Webb, Effects of 2-deoxy-D-glucose on isolated atria (1966) Mol. Pharmacol., 2, p. 77 | ||
| 504 | |a Vane, The use of isolated organs for detection of active substances in the circulatin blood (1964) Br. J. Pharmac. Chemother., 23, p. 360 | ||
| 504 | |a Goswami, Kar, Chowdhury, Uterine lipid metabolism in mice during the oestrous cycle: effect of ovariectomy and replacement therapy (1963) J. Reprod. Fert., 6, p. 287 | ||
| 504 | |a Davis, Alen, Hormonal Influence on lipid metabolism of rat uterus (1959) Anat. Rec., 134, p. 725 | ||
| 504 | |a Butcher, baird, Effects of prostaglandins on adenosine 3′5′ monophosphate levels in fat and other tissues (1968) J. Biol. Chem., 243, p. 1713 | ||
| 504 | |a Loten, Sneyd, Evidence for separate sites of action for the antilipolytic effects of insulin and prostaglandin E1 (1973) Endocrinology, 93, p. 1315 | ||
| 504 | |a Illiano, Cuatrecasas, Endogenous prostaglandins modulate lipolitic processes in adipose tissue (1971) Nature (New Biol.), 234, p. 72 | ||
| 504 | |a Waterman, Guthrie, Effects of cloprostenol administration on neutral lipid and prostaglandin F metabolism by porcine luteal tissue (1984) Prostaglandins, 27, p. 131 | ||
| 504 | |a Soulsby, Perlmutter, The role of prostaglandins E2 and F2α on canine arterial in vitro lipid biosynthesis from 14C-acetate (1982) Artery, 9, p. 342 | ||
| 520 | 3 | |a Triglyceride (TG) levels in uterine strips isolated from natural estrous, ovariectomized or ovariectomized, estradiol-injected rats, were explored. Determinations were performed either immediately after isolation (initial or 0 time) as well as after one hour period of incubation (60 min time) in glucose containing or in glucose-free solution. The influences of indomethacin alone (5×10-6M) or of ondomethacin plus prostaglandins (PGs) E2,E1 or F2α (10-7M). delivered in vitro at the beginning of the incubation, and of 2-deoxy-D-glucose 2-DG) at 11.0 mM, were also studied. Ovariectomized animals (25 days); estrous animals or spayed 17-beta estradiol-injected animals (0.5 ug + 1.0 ug, 24 hours prior to sacrifice), were employed. At 60 min time in glucose-free medium, but not in glucose containing solution, triglycerides declined significantly in strips fron spayed rats, the diminution being prevented by in vitro indomethacin. The presence of exogenous PGF2 a Or of PGE1, but not that of PGE2, abolished the pteventive effect of indomethacin. Initial TG levels in spayed uteri treated with estradiol were significantly smaller that in untreated controls. However, under these conditions, indomethacin alone or indomethacin plus the tested PGs, had no action, the same being evident at natural estrus. In strips from spayed rats and incubated in the absence of glucose but with the presence of 2-DG, the effects of indomethacin and of indomethacin plus exogenous PGs on tissue TG levels were similar, although more evident, than in the sole absence of extracellular glucose. Moreover, TG levels in uterine strips obtained from ovariectomized rats injected with 17-beta estradiol and incubated for 60 min in glucose-free medium plus 2-DG, were smaller than immediately after isolation (0 time), the decrement being prevented by indomethacin, not affected by PGE2, and abolished by PGE1, or by PGF2α The foregoing results suggest that the diminution of TG in uterine strips incubated in the absence of glucose could be due to a greater metabolic utilization of neutral fats, a phenomenon possibly associated to the lack of exogenous substrate. Moreover, this decrement of tissue TG apeears to be linked to the influence of certain PGs and of estrogens, for in ovariectomized, but not in estrous or in spayed-estrogenized conditions, indomethacin prevented the TG decrement in glucose-free solution, whereas PGs E1 and PGF2α abolished the preventive action of indomethacin. Moreover, under the combination of substrate-free condition plus the presence of 2-DG, an inhibitor of glucose transport and tissue glycolysis, uteri from both ovariectomized or spayed-estrogenized rats, reacted to the addition of indomethacin with an abolition of TG decrement, and PGs E1 or F2α, but not PGE2, blunted the influence of indomethacin. This would imply a definite influence of endogenous carbohydrate metabolism and of some PGs on tissue TG levels, both under estrogen or non-estrogen dominance. © 1987. |l eng | |
| 536 | |a Detalles de la financiación: Consejo Nacional de Investigaciones Científicas y Técnicas | ||
| 536 | |a Detalles de la financiación: E.T.Gonzilez, A.M.Franchi, A.Goldraij, M.F.Gimeno and A.L.Gimeno Centro de Estudios Farmacolhgicos y de Principios Naturales (CEFAPRIN), Consejo National de Investi-gaciones Cientificas y Tgcnicas de la Repcblica Argentina (CONICET). Serrano 665, (1414) Buenos Aires, Argentina (Supported by Grants PID 3068000/65 and PID 3007800/85 from CONICET. Reprint requests to ALG). | ||
| 593 | |a Centro de Estudios Farmacológicos y de Principios Naturales (CEFAPRIN), Consejo Nacional de Investigaciones Cientificas y Tecnicas de la Republica Argentina (CONICET). Serr, 1414 Buenos Aires, Argentina | ||
| 690 | 1 | 0 | |a DEOXYGLUCOSE |
| 690 | 1 | 0 | |a ESTRADIOL |
| 690 | 1 | 0 | |a ESTROGEN |
| 690 | 1 | 0 | |a GLUCOSE |
| 690 | 1 | 0 | |a INDOMETACIN |
| 690 | 1 | 0 | |a PROSTAGLANDIN |
| 690 | 1 | 0 | |a PROSTAGLANDIN E1 |
| 690 | 1 | 0 | |a PROSTAGLANDIN E2 |
| 690 | 1 | 0 | |a PROSTAGLANDIN F2 ALPHA |
| 690 | 1 | 0 | |a TRIACYLGLYCEROL |
| 690 | 1 | 0 | |a DEOXYGLUCOSE |
| 690 | 1 | 0 | |a DEOXYSUGAR |
| 690 | 1 | 0 | |a ESTRADIOL |
| 690 | 1 | 0 | |a ESTROGEN |
| 690 | 1 | 0 | |a GLUCOSE |
| 690 | 1 | 0 | |a INDOMETACIN |
| 690 | 1 | 0 | |a PROSTAGLANDIN |
| 690 | 1 | 0 | |a PROSTAGLANDIN E |
| 690 | 1 | 0 | |a PROSTAGLANDIN E1 |
| 690 | 1 | 0 | |a PROSTAGLANDIN E2 |
| 690 | 1 | 0 | |a PROSTAGLANDIN F |
| 690 | 1 | 0 | |a PROSTAGLANDIN F2 ALPHA |
| 690 | 1 | 0 | |a TRIACYLGLYCEROL |
| 690 | 1 | 0 | |a ANIMAL EXPERIMENT |
| 690 | 1 | 0 | |a DRUG EFFICACY |
| 690 | 1 | 0 | |a ENDOCRINE SYSTEM |
| 690 | 1 | 0 | |a FEMALE GENITAL SYSTEM |
| 690 | 1 | 0 | |a NONHUMAN |
| 690 | 1 | 0 | |a OVARIECTOMY |
| 690 | 1 | 0 | |a PRIORITY JOURNAL |
| 690 | 1 | 0 | |a RAT |
| 690 | 1 | 0 | |a SUBCUTANEOUS DRUG ADMINISTRATION |
| 690 | 1 | 0 | |a UTERUS |
| 690 | 1 | 0 | |a ANIMAL |
| 690 | 1 | 0 | |a ARTICLE |
| 690 | 1 | 0 | |a DRUG EFFECT |
| 690 | 1 | 0 | |a FEMALE |
| 690 | 1 | 0 | |a NUTRITIONAL DEFICIENCY |
| 690 | 1 | 0 | |a OVARIECTOMY |
| 690 | 1 | 0 | |a RAT STRAIN |
| 690 | 1 | 0 | |a UTERUS |
| 690 | 1 | 0 | |a ANIMALIA |
| 690 | 1 | 0 | |a ALPROSTADIL |
| 690 | 1 | 0 | |a ANIMAL |
| 690 | 1 | 0 | |a DEOXY SUGARS |
| 690 | 1 | 0 | |a DEOXYGLUCOSE |
| 690 | 1 | 0 | |a DINOPROST |
| 690 | 1 | 0 | |a DINOPROSTONE |
| 690 | 1 | 0 | |a ESTRADIOL |
| 690 | 1 | 0 | |a ESTROGENS |
| 690 | 1 | 0 | |a FEMALE |
| 690 | 1 | 0 | |a GLUCOSE |
| 690 | 1 | 0 | |a INDOMETHACIN |
| 690 | 1 | 0 | |a OVARIECTOMY |
| 690 | 1 | 0 | |a PROSTAGLANDINS |
| 690 | 1 | 0 | |a PROSTAGLANDINS E |
| 690 | 1 | 0 | |a PROSTAGLANDINS F |
| 690 | 1 | 0 | |a RATS |
| 690 | 1 | 0 | |a RATS, INBRED STRAINS |
| 690 | 1 | 0 | |a SUPPORT, NON-U.S. GOV'T |
| 690 | 1 | 0 | |a TRIGLYCERIDES |
| 690 | 1 | 0 | |a UTERUS |
| 700 | 1 | |a Franchi, A.M. | |
| 700 | 1 | |a Goldraij, A. | |
| 700 | 1 | |a Gimeno, M.F. | |
| 700 | 1 | |a Gimeno, A.L. | |
| 773 | 0 | |d 1987 |g v. 26 |h pp. 47-58 |k n. 1 |p Prostaglandins Leukotrienes Med. |x 02621746 |t Prostaglandins, Leukotrienes and Medicine | |
| 856 | 4 | 1 | |u https://www.scopus.com/inward/record.uri?eid=2-s2.0-0023163849&doi=10.1016%2f0262-1746%2887%2990151-X&partnerID=40&md5=0299a59525788ffb416f1b33d320e8d0 |y Registro en Scopus |
| 856 | 4 | 0 | |u https://doi.org/10.1016/0262-1746(87)90151-X |y DOI |
| 856 | 4 | 0 | |u https://hdl.handle.net/20.500.12110/paper_02621746_v26_n1_p47_Gonzalez |y Handle |
| 856 | 4 | 0 | |u https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_02621746_v26_n1_p47_Gonzalez |y Registro en la Biblioteca Digital |
| 961 | |a paper_02621746_v26_n1_p47_Gonzalez |b paper |c PE | ||
| 962 | |a info:eu-repo/semantics/article |a info:ar-repo/semantics/artículo |b info:eu-repo/semantics/publishedVersion | ||
| 999 | |c 79089 | ||