Measurement of specific radioactivities in labelled hormones by self-displacement analysis
A graphical method is described that allows the determination of specific radioactivities of radioactively labelled hormones. This method combines the self-displacement technique, plotting bound/free ratios versus mass of unlabelled hormone or total radioactivity of labelled preparation added to the...
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| Autor principal: | |
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| Otros Autores: | , |
| Formato: | Capítulo de libro |
| Lenguaje: | Inglés |
| Publicado: |
1983
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| Acceso en línea: | Registro en Scopus DOI Handle Registro en la Biblioteca Digital |
| Aporte de: | Registro referencial: Solicitar el recurso aquí |
| LEADER | 02756caa a22005537a 4500 | ||
|---|---|---|---|
| 001 | PAPER-18362 | ||
| 003 | AR-BaUEN | ||
| 005 | 20230518204944.0 | ||
| 008 | 190411s1983 xx ||||fo|||| 00| 0 eng|d | ||
| 024 | 7 | |2 scopus |a 2-s2.0-0020571774 | |
| 024 | 7 | |2 cas |a chorionic gonadotropin, 9002-61-3; prolactin, 12585-34-1, 50647-00-2, 9002-62-4 | |
| 040 | |a Scopus |b spa |c AR-BaUEN |d AR-BaUEN | ||
| 030 | |a BIJOA | ||
| 100 | 1 | |a Calvo, J.C. | |
| 245 | 1 | 0 | |a Measurement of specific radioactivities in labelled hormones by self-displacement analysis |
| 260 | |c 1983 | ||
| 506 | |2 openaire |e Política editorial | ||
| 520 | 3 | |a A graphical method is described that allows the determination of specific radioactivities of radioactively labelled hormones. This method combines the self-displacement technique, plotting bound/free ratios versus mass of unlabelled hormone or total radioactivity of labelled preparation added to the receptor preparation, and the maximal binding capacity of the labelled hormone. The procedure presented herein provides a more realistic specific radioactivity for use in all binding experiments. Application of the method is demonstrated for 125I-labelled ovine prolactin, and data are presented for 125I-labelled human choriogonadotropin and [3H]testosterone. |l eng | |
| 593 | |a Inst. Biol. Med. Exp., Buenos Aires (1428), Argentina | ||
| 690 | 1 | 0 | |a CHORIONIC GONADOTROPIN |
| 690 | 1 | 0 | |a CHORIONIC GONADOTROPIN I 125 |
| 690 | 1 | 0 | |a HORMONE |
| 690 | 1 | 0 | |a PROLACTIN |
| 690 | 1 | 0 | |a PROLACTIN I 125 |
| 690 | 1 | 0 | |a RADIOISOTOPE |
| 690 | 1 | 0 | |a TESTOSTERONE H 3 |
| 690 | 1 | 0 | |a UNCLASSIFIED DRUG |
| 690 | 1 | 0 | |a ANIMAL CELL |
| 690 | 1 | 0 | |a ARTICLE |
| 690 | 1 | 0 | |a ENDOCRINE SYSTEM |
| 690 | 1 | 0 | |a HORMONE BINDING |
| 690 | 1 | 0 | |a MALE GENITAL SYSTEM |
| 690 | 1 | 0 | |a METHODOLOGY |
| 690 | 1 | 0 | |a NONHUMAN |
| 690 | 1 | 0 | |a PROSTATE |
| 690 | 1 | 0 | |a RAT |
| 690 | 1 | 0 | |a ANIMALIA |
| 690 | 1 | 0 | |a OVIS |
| 700 | 1 | |a Radicella, J.P. | |
| 700 | 1 | |a Charreau, E.H. | |
| 773 | 0 | |d 1983 |g v. 212 |h pp. 259-264 |k n. 2 |p BIOCHEM. J. |x 02646021 |w (AR-BaUEN)CENRE-205 |t Biochemical Journal | |
| 856 | 4 | 1 | |u https://www.scopus.com/inward/record.uri?eid=2-s2.0-0020571774&doi=10.1042%2fbj2120259&partnerID=40&md5=232c4dd6eae29214078542e3590b416a |y Registro en Scopus |
| 856 | 4 | 0 | |u https://doi.org/10.1042/bj2120259 |y DOI |
| 856 | 4 | 0 | |u https://hdl.handle.net/20.500.12110/paper_02646021_v212_n2_p259_Calvo |y Handle |
| 856 | 4 | 0 | |u https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_02646021_v212_n2_p259_Calvo |y Registro en la Biblioteca Digital |
| 961 | |a paper_02646021_v212_n2_p259_Calvo |b paper |c PE | ||
| 962 | |a info:eu-repo/semantics/article |a info:ar-repo/semantics/artículo |b info:eu-repo/semantics/publishedVersion | ||
| 963 | |a VARI | ||
| 999 | |c 79315 | ||