The placenta as a glucocorticoid target tissue: Exchange assay of (3H)-dexamethasone binding and effect of steroids on receptor content
Binding of (3H)-dexamethasone was studied in high speed supernatant (HSS) of basal zone trophoblast and labyrinthine zone of rat placenta using an exchange assay. The system showed the following characteristics: maximum binding was attained after 120 min of incubation; molybdate was required in the...
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| Otros Autores: | , |
| Formato: | Capítulo de libro |
| Lenguaje: | Inglés |
| Publicado: |
1983
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| Materias: | |
| Acceso en línea: | Registro en Scopus Handle Registro en la Biblioteca Digital |
| Aporte de: | Registro referencial: Solicitar el recurso aquí |
| LEADER | 04331caa a22007577a 4500 | ||
|---|---|---|---|
| 001 | PAPER-18364 | ||
| 003 | AR-BaUEN | ||
| 005 | 20230518204944.0 | ||
| 008 | 190411s1983 xx ||||fo|||| 00| 0 eng|d | ||
| 024 | 7 | |2 scopus |a 2-s2.0-0020700061 | |
| 024 | 7 | |2 cas |a corticosterone, 50-22-6; dexamethasone, 50-02-2; estradiol, 50-28-2; progesterone, 57-83-0; testosterone, 58-22-0; Corticosterone, 50-22-6; dexamethasone receptor; Dexamethasone, 50-02-2; Estradiol, 50-28-2; Progesterone, 57-83-0; Receptors, Glucocorticoid; Receptors, Steroid; Testosterone, 57-85-2; Tritium, 10028-17-8 | |
| 040 | |a Scopus |b spa |c AR-BaUEN |d AR-BaUEN | ||
| 030 | |a HMMRA | ||
| 100 | 1 | |a Heller, C. | |
| 245 | 1 | 4 | |a The placenta as a glucocorticoid target tissue: Exchange assay of (3H)-dexamethasone binding and effect of steroids on receptor content |
| 260 | |c 1983 | ||
| 506 | |2 openaire |e Política editorial | ||
| 520 | 3 | |a Binding of (3H)-dexamethasone was studied in high speed supernatant (HSS) of basal zone trophoblast and labyrinthine zone of rat placenta using an exchange assay. The system showed the following characteristics: maximum binding was attained after 120 min of incubation; molybdate was required in the medium to measure binding sites at 20°C and equilibrium conditions; exchange was near 90-100% between ligand and corticosterone added during a preincubation; (4) low capacity (190-250 fmoles/mg protein), high affinity (K(d) 10-8 M) binders were determined by saturation analysis; competition with other steroids in vitro revealed that in basal zone trophoblast, only dexamethasone displaced the ligand, whereas in labyrinthine zone corticosterone, progesterone and testosterone competed to a smaller degree than dexamethasone. We have also studied the effect of in vivo treatment of pregnant rats for four days with dexamethasone, corticosterone, estradiol, progesterone or testosterone, on (3H)-dexamethasone binding. Of all tested compounds, only dexamethasone treatment (which was suspended 24 h before the experiment to prevent receptor occupancy by exogenous hormone) significantly decreased binding of the tritiated hormone in HSS of labyrinthine zone and basal zone trophoblast. These results suggest that dexamethasone regulates its own receptor in placenta. The physiological aspect of this phenomenon requires elucidation of the role of glucocorticoids on placental function in the rat. |l eng | |
| 593 | |a Lab. Esteroides, Inst. Biol. Med. Exp., 1428 Buenos Aires, Argentina | ||
| 690 | 1 | 0 | |a CORTICOSTERONE |
| 690 | 1 | 0 | |a DEXAMETHASONE |
| 690 | 1 | 0 | |a ESTRADIOL |
| 690 | 1 | 0 | |a GLUCOCORTICOID |
| 690 | 1 | 0 | |a PROGESTERONE |
| 690 | 1 | 0 | |a RADIOISOTOPE |
| 690 | 1 | 0 | |a STEROID |
| 690 | 1 | 0 | |a TESTOSTERONE |
| 690 | 1 | 0 | |a ANIMAL CELL |
| 690 | 1 | 0 | |a DEXAMETHASONE H 3 |
| 690 | 1 | 0 | |a DOSE RESPONSE |
| 690 | 1 | 0 | |a DRUG BINDING |
| 690 | 1 | 0 | |a DRUG COMPARISON |
| 690 | 1 | 0 | |a DRUG RECEPTOR BINDING |
| 690 | 1 | 0 | |a DRUG RESPONSE |
| 690 | 1 | 0 | |a FETUS |
| 690 | 1 | 0 | |a NONHUMAN |
| 690 | 1 | 0 | |a PHARMACOKINETICS |
| 690 | 1 | 0 | |a PREGNANCY |
| 690 | 1 | 0 | |a RAT |
| 690 | 1 | 0 | |a TARGET |
| 690 | 1 | 0 | |a ANIMAL |
| 690 | 1 | 0 | |a BINDING SITES |
| 690 | 1 | 0 | |a CORTICOSTERONE |
| 690 | 1 | 0 | |a DEXAMETHASONE |
| 690 | 1 | 0 | |a ESTRADIOL |
| 690 | 1 | 0 | |a FEMALE |
| 690 | 1 | 0 | |a PREGNANCY |
| 690 | 1 | 0 | |a PROGESTERONE |
| 690 | 1 | 0 | |a RATS |
| 690 | 1 | 0 | |a RATS, INBRED STRAINS |
| 690 | 1 | 0 | |a RECEPTORS, GLUCOCORTICOID |
| 690 | 1 | 0 | |a RECEPTORS, STEROID |
| 690 | 1 | 0 | |a SUPPORT, NON-U.S. GOV'T |
| 690 | 1 | 0 | |a TESTOSTERONE |
| 690 | 1 | 0 | |a TRITIUM |
| 650 | 1 | 7 | |2 spines |a PLACENTA |
| 650 | 1 | 7 | |2 spines |a PLACENTA |
| 700 | 1 | |a Coirini, H. | |
| 700 | 1 | |a De Nicola, A.F. | |
| 773 | 0 | |d 1983 |g v. 15 |h pp. 41-45 |k n. 1 |p HORM. METAB. RES. |x 00185043 |t Hormone and Metabolic Research | |
| 856 | 4 | 1 | |u https://www.scopus.com/inward/record.uri?eid=2-s2.0-0020700061&partnerID=40&md5=76b9c79f0244ce03e712fddc12d2c131 |y Registro en Scopus |
| 856 | 4 | 0 | |u https://hdl.handle.net/20.500.12110/paper_00185043_v15_n1_p41_Heller |y Handle |
| 856 | 4 | 0 | |u https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00185043_v15_n1_p41_Heller |y Registro en la Biblioteca Digital |
| 961 | |a paper_00185043_v15_n1_p41_Heller |b paper |c PE | ||
| 962 | |a info:eu-repo/semantics/article |a info:ar-repo/semantics/artículo |b info:eu-repo/semantics/publishedVersion | ||
| 999 | |c 79317 | ||