Binding of [3H]dexamethasone by rat placenta
[3H]Dexamethasone binding was measured in the basal zone trophoblast (BZT) and the labyrinthine zone (LZ) of placenta from Wistar rats on days 19–22 of pregnancy. Cytosol from both zones was incubated with [3H]dexamethasone for 4 h at 0–4 C, and high affinity, low capacity sites were measured by Sca...
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| Formato: | Capítulo de libro |
| Lenguaje: | Inglés |
| Publicado: |
1981
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| Acceso en línea: | Registro en Scopus DOI Handle Registro en la Biblioteca Digital |
| Aporte de: | Registro referencial: Solicitar el recurso aquí |
| LEADER | 09419caa a22012497a 4500 | ||
|---|---|---|---|
| 001 | PAPER-18484 | ||
| 003 | AR-BaUEN | ||
| 005 | 20230518204951.0 | ||
| 008 | 190411s1981 xx ||||fo|||| 00| 0 eng|d | ||
| 024 | 7 | |2 scopus |a 2-s2.0-0019732751 | |
| 024 | 7 | |2 cas |a dexamethasone, 50-02-2; mercaptoethanol, 60-24-2; n ethylmaleimide, 128-53-0; tritium, 10028-17-8; corticosterone, 50-22-6; estradiol, 50-28-2; progesterone, 57-83-0; promegestone, 34184-77-5; testosterone, 58-22-0; Dexamethasone, 50-02-2; Ethylmaleimide, 128-53-0; Mercaptoethanol, 60-24-2; Receptors, Glucocorticoid; Receptors, Steroid; Tritium, 10028-17-8 | |
| 040 | |a Scopus |b spa |c AR-BaUEN |d AR-BaUEN | ||
| 100 | 1 | |a Heller, C. | |
| 245 | 1 | 0 | |a Binding of [3H]dexamethasone by rat placenta |
| 260 | |c 1981 | ||
| 506 | |2 openaire |e Política editorial | ||
| 504 | |a Guerné, J.M., Stutinsky, F., Estradiol and progesterone binding in rabbit placenta during gestation (1978) Horm Metab Res, 10, p. 548 | ||
| 504 | |a Mc Cormack, S.A., Glasser, S.R., Ontogeny and regulation of a rat placental estrogen receptor (1978) Endocrinology, 102, p. 273 | ||
| 504 | |a Speeg, K.V., Jr., Harrison, R.W., The ontogeny of the human placental glucocorticoid receptor and inducibility of heat-stable alkaline phosphatase (1979) Endocrinology, 194, p. 1364 | ||
| 504 | |a Dancis, J., Jansen, V., Levitz, M., Placental transfer of steroids: Effect of binding to serum albumin and to placenta (1980) Am J Physiol, 238, p. 208 | ||
| 504 | |a Giannopoulos, G., Hanzzan, A., Solomon, S., Glucocorticoid receptors in fetal and adult rabbit tissues (1974) J Biol Chem, 249, p. 2424 | ||
| 504 | |a Wong, M.D., Burton, A.F., Studies on corticosterone-receptor complexes from mouse placenta (1974) Can J Biochem, 52, p. 190 | ||
| 504 | |a Wolf, G., Rosner, W., Investigation of a corticosteroid-binding globulin-like molecule in human placenta (1979) J Clin Endocrinol Metab, 49, p. 269 | ||
| 504 | |a Rousseau, G.G., Baxter, J.D., Tomkins, G.M., Glucocorticoid receptor: Relations between steroid binding and biologic effects (1972) J Mol Biol, 67, p. 99 | ||
| 504 | |a Donaldson, H.H., The Rat, Data and Reference Tables, ed 2. Memoirs of the Wistar Institute of Anatomy and Biology (1924) Philadelphia, p. 172 | ||
| 504 | |a Parkes, A.S., (1952) Marshall’s Physiology of Reproduction, p. 258. , Longmans, Green, and Co, London | ||
| 504 | |a Mc Cormack, S.A., Glasser, S.R., A high-affinity estrogen-binding protein in rat placental trophoblast (1976) Endocrinology, 99, p. 701 | ||
| 504 | |a Baxter, J.D., Tomkins, G.M., Specific cytoplasmic glucocorticoid hormone receptors in hepatoma tissue culture cells (1971) Proc Natl Acad Sci USA, 68, p. 932 | ||
| 504 | |a Fridman, O., Foglia, V.G., De Nicola, A.F., Reduction in (3H) corticosterone binding to cytoplasmic receptors in the brain of diabetic rats (1978) J Steroid Biochem, 9, p. 609 | ||
| 504 | |a Anderson, J.N., Peck, E.J., Clark, J.H., Nuclear receptor estrogen complex: Accumulation, retention and localization in the hypothalamus and pituitary (1973) Endocrinology, 93, p. 711 | ||
| 504 | |a Mc Ewen, B.S., Zigmond, R.E., Isolation of brain cell nuclei (1972) Research Methods in Neurochemistry., 1, p. 140. , Marks N, Rodnigh R (eds), Plenum Press, New York | ||
| 504 | |a Lowry, O.H., Rosebrough, N.J., Farr, A.L., Randall, R.J., Protein measurement with the Folin phenol reagent (1951) J Biol Chem, 193, p. 265 | ||
| 504 | |a Burton, K., A study of conditions and mechanism of the di-phenylamine reaction for the colorimetric estimation of deoxyribonucleic acid (1956) Biochem J, 62, p. 315 | ||
| 504 | |a Ogle, T.F., Characteristics of high affinity progesterone binding to rat placental cytosol (1980) Endocrinology, 106, p. 1861 | ||
| 504 | |a Maclusky, N.J., Chaptal, C., Lieberburg, I., Mc Ewen, B.S., Properties and subcellular inter-relationships of presumptive estrogen receptor macromolecules in the brains of neonatal and prepuberal female rats (1976) Brain Res, 114, p. 158 | ||
| 504 | |a Clark, J.H., Peck, E.J., Nuclear retention of receptor-oestrogen complex and nuclear acceptor sites (1976) Nature, 260, p. 635 | ||
| 504 | |a Dupouy, J.P., Coffigny, H., Magre, S., Maternal and foetal corticosterone levels during late pregnancy in rats (1975) J Endocrinol, 65, p. 347 | ||
| 504 | |a Cake, M.H., Litwack, G., The glucorticoid receptor (1975) Biochemical Actions of Hormones, p. 317. , Litwack G, Academic Press, New York | ||
| 504 | |a Liggins, G.C., Fairclough, R.J., Grieves, S.A., Kendall, J.Z., Knox, B.S., The mechanism of initiation of parturition in the ewe (1973) Recent Prog Horm Res, 29, p. 111 | ||
| 504 | |a Blackburn, W.R., Kaplan, H.S., Mc Kay, D.G., Morphologic changes in the developing rat placenta following prednisolone administration (1965) Am J Obstet Gynecol, 92, p. 234 | ||
| 504 | |a Barlow, S.M., Morrison, P.J., Sullivan, F.M., Plasma corticosterone levels during pregnancy in the mouse: The relative contributions of the adrenal glands and foeto-placental units (1974) J Endocrinol, 60, p. 473 | ||
| 504 | |a Thorburn, G.D., Challis, J., Endocrine control of parturition (1979) Physiol Rev, 59, p. 863 | ||
| 520 | 3 | |a [3H]Dexamethasone binding was measured in the basal zone trophoblast (BZT) and the labyrinthine zone (LZ) of placenta from Wistar rats on days 19–22 of pregnancy. Cytosol from both zones was incubated with [3H]dexamethasone for 4 h at 0–4 C, and high affinity, low capacity sites were measured by Scatchard plot analysis in BZT and LZ, tissues which demonstrated similar concentrations of sites and affinity constants. Specific binding was decreased by omitting mercaptoethanol from the incubation buffer, by adding N-ethylmaleimide, or by increasing the temperature to 16 or 37 C. Competition studies revealed that the rank order of inhibition of [3H]dexamethasone binding by nonradioactive steroids was dexamethasone ≫ progesterone = corticosterone ≫ testosterone = 17α-estradiol. Dexamethasone-binding sites could be distinguished from progestinbinding sites by competition analysis as well as by the absence of 17α, 21-dimethyl-19-nor-pregna-4, 9-diene-3, 20-dione ([3H]-R5020) binding in LZ, whereas the corticoid was bound to both zones. Nuclei from BZT and LZ took up radioactive steroid when exposed to cytosol prelabeled with [3H]dexamethasone, but little radioactivity was present in nuclei incubated with buffer containing [3H]dexamethasone. After in vivo administration of [3H]dexamethasone to pregnant rats, radioactivity was recovered in nuclei and cytosol from both placental zones. Furthermore, whole homogenates or cytosol from BZT or LZ did not metabolize [3H]dexamethasone to any significant extent. These results suggest that rat placenta contains dexamethasonebinding sites which resemble the glucocorticoid receptors described in other tissues. © 1981 by The Endocrine Society. |l eng | |
| 593 | |a Instituto de Biologia y Medicina Experimental, Obligado 2490, Buenos Aires, 1428, Argentina | ||
| 690 | 1 | 0 | |a DEXAMETHASONE |
| 690 | 1 | 0 | |a GLUCOCORTICOID RECEPTOR |
| 690 | 1 | 0 | |a MERCAPTOETHANOL |
| 690 | 1 | 0 | |a N ETHYLMALEIMIDE |
| 690 | 1 | 0 | |a STEROID RECEPTOR |
| 690 | 1 | 0 | |a TRITIUM |
| 690 | 1 | 0 | |a CORTICOSTERONE |
| 690 | 1 | 0 | |a DEXAMETHASONE |
| 690 | 1 | 0 | |a DRUG RECEPTOR |
| 690 | 1 | 0 | |a ESTRADIOL |
| 690 | 1 | 0 | |a HORMONE RECEPTOR |
| 690 | 1 | 0 | |a N ETHYLMALEIMIDE |
| 690 | 1 | 0 | |a PROGESTERONE |
| 690 | 1 | 0 | |a PROMEGESTONE |
| 690 | 1 | 0 | |a RADIOISOTOPE |
| 690 | 1 | 0 | |a TESTOSTERONE |
| 690 | 1 | 0 | |a ANIMAL |
| 690 | 1 | 0 | |a ARTICLE |
| 690 | 1 | 0 | |a BINDING COMPETITION |
| 690 | 1 | 0 | |a CELL NUCLEUS |
| 690 | 1 | 0 | |a CYTOSOL |
| 690 | 1 | 0 | |a FEMALE |
| 690 | 1 | 0 | |a KINETICS |
| 690 | 1 | 0 | |a METABOLISM |
| 690 | 1 | 0 | |a PREGNANCY |
| 690 | 1 | 0 | |a RAT |
| 690 | 1 | 0 | |a ANIMAL EXPERIMENT |
| 690 | 1 | 0 | |a CORTICOSTERONE H 3 |
| 690 | 1 | 0 | |a DEXAMETHASONE H 3 |
| 690 | 1 | 0 | |a ENDOCRINE SYSTEM |
| 690 | 1 | 0 | |a FEMALE GENITAL SYSTEM |
| 690 | 1 | 0 | |a PREGNANCY |
| 690 | 1 | 0 | |a PROMEGESTONE H 3 |
| 690 | 1 | 0 | |a ANIMAL |
| 690 | 1 | 0 | |a BINDING, COMPETITIVE |
| 690 | 1 | 0 | |a CELL NUCLEUS |
| 690 | 1 | 0 | |a CYTOSOL |
| 690 | 1 | 0 | |a DEXAMETHASONE |
| 690 | 1 | 0 | |a ETHYLMALEIMIDE |
| 690 | 1 | 0 | |a FEMALE |
| 690 | 1 | 0 | |a KINETICS |
| 690 | 1 | 0 | |a MERCAPTOETHANOL |
| 690 | 1 | 0 | |a PREGNANCY |
| 690 | 1 | 0 | |a RATS |
| 690 | 1 | 0 | |a RECEPTORS, GLUCOCORTICOID |
| 690 | 1 | 0 | |a RECEPTORS, STEROID |
| 690 | 1 | 0 | |a SUPPORT, NON-U.S. GOV'T |
| 690 | 1 | 0 | |a TRITIUM |
| 650 | 1 | 7 | |2 spines |a PLACENTA |
| 650 | 1 | 7 | |2 spines |a PLACENTA |
| 650 | 1 | 7 | |2 spines |a PLACENTA |
| 653 | 0 | 0 | |a r 5020 |
| 700 | 1 | |a Coirini, H. | |
| 700 | 1 | |a De Nicola, A.F. | |
| 773 | 0 | |d 1981 |g v. 108 |h pp. 1697-1702 |k n. 5 |p Endocrinology |x 00137227 |w (AR-BaUEN)CENRE-4573 |t Endocrinology | |
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| 856 | 4 | 0 | |u https://doi.org/10.1210/endo-108-5-1697 |y DOI |
| 856 | 4 | 0 | |u https://hdl.handle.net/20.500.12110/paper_00137227_v108_n5_p1697_Heller |y Handle |
| 856 | 4 | 0 | |u https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00137227_v108_n5_p1697_Heller |y Registro en la Biblioteca Digital |
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