Characterization of inositolphospholipids in Tryparzosoma cruzi trypmastigote forms
In vivo labeling experiments with [3H]palmitic acid, [3H]inositol, and [3H]glucose allowed the identification of two main classes of inositolphospholipids (IPLs) from the trypomastigote stage of Trypanosoma cruzi. Purification of these compounds was achieved by ion-exchange chromatography, high perf...
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| Formato: | Capítulo de libro |
| Lenguaje: | Inglés |
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Elsevier B.V.
1996
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| Acceso en línea: | Registro en Scopus DOI Handle Registro en la Biblioteca Digital |
| Aporte de: | Registro referencial: Solicitar el recurso aquí |
| LEADER | 09244caa a22012737a 4500 | ||
|---|---|---|---|
| 001 | PAPER-18873 | ||
| 003 | AR-BaUEN | ||
| 005 | 20230518205014.0 | ||
| 008 | 190411s1996 xx ||||fo|||| 00| 0 eng|d | ||
| 024 | 7 | |2 scopus |a 2-s2.0-0030594465 | |
| 024 | 7 | |2 cas |a Ceramides; Diglycerides; Fatty Acids; glycerylphosphoinositol, 16824-65-0; Glycosylphosphatidylinositols; Inositol Phosphates; Palmitic Acid, 57-10-3; Palmitic Acids; Phosphatidylinositols; Sphingosine, 123-78-4 | |
| 040 | |a Scopus |b spa |c AR-BaUEN |d AR-BaUEN | ||
| 030 | |a BBLLA | ||
| 100 | 1 | |a Uhrig, M.L. | |
| 245 | 1 | 0 | |a Characterization of inositolphospholipids in Tryparzosoma cruzi trypmastigote forms |
| 260 | |b Elsevier B.V. |c 1996 | ||
| 270 | 1 | 0 | |m De Lederkremer, R.M.; Departamento de Quimica Organica, Facultad Ciencias Exactas/Naturales, Universidad de Buenos Aires, Pabellon 2, Buenos Aires, Argentina |
| 506 | |2 openaire |e Política editorial | ||
| 504 | |a Brener, Z., (1973) Annu. Rev. Microbiol., 27, pp. 347-383 | ||
| 504 | |a Contreras, V.T., Salles, J.M., Thomas, N., Morel, C.M., Goldemberg, S., (1985) Mol. Biochem. Parasitol., 16, pp. 315-327 | ||
| 504 | |a McConville, M.J., Ferguson, M.A.J., (1993) Biochem. J., 294, pp. 305-324 | ||
| 504 | |a Doering, T.L., Pessin, M.S., Hart, G.W., Raben, D.M., Englund, P.T., (1994) Biochem. J., 299, pp. 741-746 | ||
| 504 | |a Masterson, W.J., Raper, J., Doering, T.L., Hart, G.W., Englund, P.T., (1990) Cell, 62, pp. 73-80 | ||
| 504 | |a Buxbaum, L.U., Raper, J., Opperdoes, F.R., Englund, P.T., (1994) J. Biol. Chem., 269, pp. 30212-30220 | ||
| 504 | |a Heise, N., Cardoso De Almeida, M.L., Ferguson, M.A.J., (1995) Molec. and Biochem. Parasitol., 70, pp. 71-84 | ||
| 504 | |a Couto, A.S., Lederkremer, R.M., Colli, W., Alves, M.J.M., (1993) Eur. J. Biochem., 217, pp. 597-602 | ||
| 504 | |a Schenkman, S., Ferguson, M.A.J., Heise, N., Cardoso De Almeida, M.L., Mortara, R.A., Yoshida, N., (1993) Mol. Biochem. Parasitol., 59, pp. 293-304 | ||
| 504 | |a Lederkremer, R.M., Lima, C., Ramirez, M.I., Ferguson, M.A.J., Homans, S.W., Thomas-Oates, J., (1991) J. Biol. Chem., 266, pp. 23670-23675 | ||
| 504 | |a Previato, J.O., Gorin, P.A.J., Mazurek, M., Xavier, M.T., Fournet, B., Wieruszesk, J.M., Mendonça-Previato, L., (1990) J. Biol. Chem., 265, pp. 2518-2526 | ||
| 504 | |a Lederkremer, R.M., Casal, O.L., Tanaka, C.T., Colli, W., (1978) Biochem. Biophys. Res. Commun., 85, pp. 1268-1274 | ||
| 504 | |a Lederkremer, R.M., Lima, C., Ramirez, M.I., Casal, O.L., (1990) Eur. J. Biochem., 192, pp. 337-345 | ||
| 504 | |a Lederkremer, R.M., Lima, C., Ramirez, M.I., Gonçalvez, M.F., Colli, W., (1993) Eur. J. Biochem., 218, pp. 929-936 | ||
| 504 | |a Conzelmann, A., Puoti, A., Lester, R.L., Desponds, C., (1992) Embo J., 11, pp. 457-466 | ||
| 504 | |a Sipos, G., Puoti, A., Conzelmann, A., (1994) Embo J., 13, pp. 2789-2796 | ||
| 504 | |a Bertello, L.E., Gonçalvez, M.F., Colli, W., Lederkremer, R.M., (1995) Biochem. J., 310, pp. 255-261 | ||
| 504 | |a Andrews, N.W., Colli, W., (1982) J. Protozool., 29, pp. 264-269 | ||
| 504 | |a Uhrig, M.L., Couto, A.S., Lederkremer, R.M., Zingales, B., Colli, W., (1992) Carbohydr. Res., 231, pp. 329-334 | ||
| 504 | |a Weiss, B., Stiller, R.L., Jack, R.C.M., (1973) Lipids, 8, pp. 25-30 | ||
| 504 | |a Manku, M.S., (1983) J. Chromatogr. Sci., 21, pp. 367-369 | ||
| 504 | |a Da Silveira, J.F., Colli, W., (1981) Biophys. Biochem. Acta, 644, pp. 341-350 | ||
| 504 | |a Sjursnes, B.J., Anthonsen, T., (1994) Biocatalysis, 9, pp. 285-297 | ||
| 504 | |a Kaneshiro, E.S., Jayasimhulu, K., Lester, R.L., (1986) J. Lipid Res., 27, pp. 1294-1303 | ||
| 504 | |a Sing, B.N., Costello, C.E., Beach, D.H., Holz Jr., G.G., (1988) Biochem. Biophys. Res. Commun., 157, pp. 1239-1246 | ||
| 504 | |a Patnaik, P.K., Field, M.C., Menon, A.K., Cross, G.A.M., Yee, M.C., Bütikofer, P., (1993) Mol. Biochem. Parasitol., 58, pp. 97-106 | ||
| 504 | |a Güther, M.L.S., Cardoso De Almeida, M.L., Yoshida, N., Ferguson, M.A.J., (1992) J. Biol. Chem., 267, pp. 6820-6828 | ||
| 504 | |a Previato, J.O., Jones, C., Xavier, M.T., Wait, R., Travassos, L.R., Parodi, A.J., Mendonça-Previato, L., (1995) J. Biol. Chem., 270, pp. 7241-7250 | ||
| 520 | 3 | |a In vivo labeling experiments with [3H]palmitic acid, [3H]inositol, and [3H]glucose allowed the identification of two main classes of inositolphospholipids (IPLs) from the trypomastigote stage of Trypanosoma cruzi. Purification of these compounds was achieved by ion-exchange chromatography, high performance liquid chromatography and thin layer chromatography. Specific phosphatidyl-inositol phospholipase C digestion, dephosphorylation and acid methanolysis showed a ceramide structure for the lower migrating IPL1. Palmitoyldihydrosphingosine and palmitoylsphingosine were detected by reverse-phase thin-layer chromatography. On the other hand, IPL2 showed to be a mixture of diacylglycero- and alkylacylglycero-phospholipids in a 1:1 ratio. After PI-PLC digestion, the lipids were separated by preparative TLC and individually analysed. The diacylglycerol contained mainly C18:0 fatty acid together with a low amount of C16:0. Hexadecylglycerol esterified with the C18:0 fatty acid was the only alkylacylglycerol detected. The C18:2 and C18:1 fatty acids, preponderant in the PI molecules of epimastigote forms, were not detected in trypomastigote forms. This is the first report on inositol phospholipids, putative precursors of lipid anchors in the infective stage of T. cruzi. |l eng | |
| 536 | |a Detalles de la financiación: Consejo Nacional de Investigaciones Científicas y Técnicas | ||
| 536 | |a Detalles de la financiación: Universidad de Buenos Aires | ||
| 536 | |a Detalles de la financiación: Fundação de Amparo à Pesquisa do Estado de São Paulo, Tecnol6gico | ||
| 536 | |a Detalles de la financiación: This investigation received financial support from UNDP/World Bank/World Health Organization Special Programme for Research and Training in Tropical Diseases, Consejo Nacional de Investigaciones Cientificas y T6cnicas (CONICET) and Universidad de Buenos Aires to R.M.L.; Fundaci6n Antorchas/Vitae to R.M.L. and W.C., Funda~o de Amparo a Pesquisa do Estado de S~o Paulo and Conselho Nacional de Desenvolvimento Cientffico e Tecnol6gico to W.C.R.M.L. and A.S.C. are research members of CONICET. We are grateful to Dr. Martin G. Low for the gift of PI-PLC from B. thuringiensis and Laura Bertello for a sample of purified IPLs from epi-mastigote cells and alkylacylglycerols. | ||
| 593 | |a Depto. de Quim. Orgánica, Universidad de Buenos Aires, Ciudad Universitaria, 1428, Buenos Aires, Argentina | ||
| 593 | |a Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, São Paulo, Brazil | ||
| 690 | 1 | 0 | |a INOSITOLPHOSPHOLIPID |
| 690 | 1 | 0 | |a T. CRUZI |
| 690 | 1 | 0 | |a TRYPOMASTIGOTE |
| 690 | 1 | 0 | |a INOSITOL DERIVATIVE |
| 690 | 1 | 0 | |a PHOSPHOLIPID |
| 690 | 1 | 0 | |a CERAMIDE |
| 690 | 1 | 0 | |a DIACYLGLYCEROL |
| 690 | 1 | 0 | |a FATTY ACID |
| 690 | 1 | 0 | |a GLYCERYLPHOSPHOINOSITOL |
| 690 | 1 | 0 | |a GLYCOSYLPHOSPHATIDYLINOSITOL |
| 690 | 1 | 0 | |a INOSITOL PHOSPHATE |
| 690 | 1 | 0 | |a PALMITIC ACID |
| 690 | 1 | 0 | |a PALMITIC ACID DERIVATIVE |
| 690 | 1 | 0 | |a PHOSPHATIDYLINOSITOL |
| 690 | 1 | 0 | |a SPHINGOSINE |
| 690 | 1 | 0 | |a ARTICLE |
| 690 | 1 | 0 | |a HIGH PERFORMANCE LIQUID CHROMATOGRAPHY |
| 690 | 1 | 0 | |a ION EXCHANGE CHROMATOGRAPHY |
| 690 | 1 | 0 | |a ISOTOPE LABELING |
| 690 | 1 | 0 | |a LIFE CYCLE |
| 690 | 1 | 0 | |a LIPID ANALYSIS |
| 690 | 1 | 0 | |a PRIORITY JOURNAL |
| 690 | 1 | 0 | |a THIN LAYER CHROMATOGRAPHY |
| 690 | 1 | 0 | |a TRYPANOSOMA CRUZI |
| 690 | 1 | 0 | |a ANIMAL |
| 690 | 1 | 0 | |a CHEMICAL STRUCTURE |
| 690 | 1 | 0 | |a CHEMISTRY |
| 690 | 1 | 0 | |a ISOLATION AND PURIFICATION |
| 690 | 1 | 0 | |a METABOLISM |
| 690 | 1 | 0 | |a TRYPANOSOMA |
| 690 | 1 | 0 | |a TRYPANOSOMA CRUZI |
| 690 | 1 | 0 | |a ANIMALS |
| 690 | 1 | 0 | |a CERAMIDES |
| 690 | 1 | 0 | |a CHROMATOGRAPHY, HIGH PRESSURE LIQUID |
| 690 | 1 | 0 | |a CHROMATOGRAPHY, THIN LAYER |
| 690 | 1 | 0 | |a DIGLYCERIDES |
| 690 | 1 | 0 | |a FATTY ACIDS |
| 690 | 1 | 0 | |a GLYCOSYLPHOSPHATIDYLINOSITOLS |
| 690 | 1 | 0 | |a INOSITOL PHOSPHATES |
| 690 | 1 | 0 | |a MOLECULAR STRUCTURE |
| 690 | 1 | 0 | |a PALMITIC ACID |
| 690 | 1 | 0 | |a PALMITIC ACIDS |
| 690 | 1 | 0 | |a PHOSPHATIDYLINOSITOLS |
| 690 | 1 | 0 | |a SPHINGOSINE |
| 690 | 1 | 0 | |a TRYPANOSOMA CRUZI |
| 700 | 1 | |a Couto, A.S. | |
| 700 | 1 | |a Colli, W. | |
| 700 | 1 | |a De Lederkremer, R.M. | |
| 773 | 0 | |d Elsevier B.V., 1996 |g v. 1300 |h pp. 233-239 |k n. 3 |p BIOCHIM. BIOPHYS. ACTA LIPIDS LIPID METAB. |x 00052760 |w (AR-BaUEN)CENRE-2261 |t Biochimica et Biophysica Acta - Lipids and Lipid Metabolism | |
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| 856 | 4 | 0 | |u https://doi.org/10.1016/0005-2760(96)00021-5 |y DOI |
| 856 | 4 | 0 | |u https://hdl.handle.net/20.500.12110/paper_00052760_v1300_n3_p233_Uhrig |y Handle |
| 856 | 4 | 0 | |u https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00052760_v1300_n3_p233_Uhrig |y Registro en la Biblioteca Digital |
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