Metabolites from the Dark Septate Endophyte Drechslera sp. Evaluation by LC/MS and Principal Component Analysis of Culture Extracts with Histone Deacetylase Inhibitors
Secondary metabolites from the cultures of the dark septate fungal endophyte (DSE) Drechslera sp., isolated from the roots of rye grass (Lollium sp.) and cultured under different experimental conditions, are described here for the first time. The use of suberoylanilidehydroxamic acid (SAHA) and othe...
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Wiley-VCH Verlag
2018
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| LEADER | 17535caa a22017417a 4500 | ||
|---|---|---|---|
| 001 | PAPER-25114 | ||
| 003 | AR-BaUEN | ||
| 005 | 20230518205706.0 | ||
| 008 | 190410s2018 xx ||||fo|||| 00| 0 eng|d | ||
| 024 | 7 | |2 scopus |a 2-s2.0-85050730831 | |
| 024 | 7 | |2 cas |a piperazinedione, 29990-68-9; vorinostat, 149647-78-9; histone deacetylase, 9076-57-7; Antifungal Agents; Histone Deacetylase Inhibitors; Histone Deacetylases | |
| 040 | |a Scopus |b spa |c AR-BaUEN |d AR-BaUEN | ||
| 030 | |a CBHIA | ||
| 100 | 1 | |a Siless, G.E. | |
| 245 | 1 | 0 | |a Metabolites from the Dark Septate Endophyte Drechslera sp. Evaluation by LC/MS and Principal Component Analysis of Culture Extracts with Histone Deacetylase Inhibitors |
| 260 | |b Wiley-VCH Verlag |c 2018 | ||
| 506 | |2 openaire |e Política editorial | ||
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| 520 | 3 | |a Secondary metabolites from the cultures of the dark septate fungal endophyte (DSE) Drechslera sp., isolated from the roots of rye grass (Lollium sp.) and cultured under different experimental conditions, are described here for the first time. The use of suberoylanilidehydroxamic acid (SAHA) and other histone deacetylase inhibitors as epigenetic modifiers in the culture medium was evaluated by LC/MS and LC/MS/MS. Several differences in the metabolite production were detected by means of supervised principal component analysis (PCA) of LC/MS data. The presence of the compounds in the culture medium or in the mycelium was compared. In order to confirm their structure, many of these natural products were isolated from a larger scale culture. These metabolites were characterized as prenylhydroxybenzoic acids and chromans, two compounds, one of each class were previously undescribed, prenylquinoids, diketopiperazines and macrosphelides. Some of the compounds, which were released to the medium, showed good antifungal activity, suggesting that these compounds could protect Lollium from fungal phytopatogens. The use of SAHA as an additive of the cultures also induced the release of hexosylphytosphyngosine to the culture medium. The biotransformation of the inhibitors was observed in addition to the production of antifungal metabolites, showing the ability of this endophytic strain to control xenobiotics. © 2018 Wiley-VHCA AG, Zurich, Switzerland |l eng | |
| 536 | |a Detalles de la financiación: Agencia Nacional de Promoción Científica y Tecnológica, PICT 2008-1394 | ||
| 536 | |a Detalles de la financiación: Consejo Nacional de Investigaciones Científicas y Técnicas, PIP 100516 | ||
| 536 | |a Detalles de la financiación: and 100352), CONICET (PIP 100516) and ANPCyT (PICT 2008-1394) for partial financial support. | ||
| 593 | |a Universidad de Buenos Aires, Facultad de Ciencias Exactas y Naturales, Departamento de Química Orgánica, Buenos Aires, Argentina | ||
| 593 | |a CONICET-Universidad de Buenos Aires, Unidad de Microanálisis y Métodos Físicos aplicados a la Química Orgánica (UMYMFOR), Buenos Aires, Argentina | ||
| 593 | |a Universidad de Buenos Aires, Facultad de Ciencias Exactas y Naturales, Departamento de Biodiversidad y Biología Experimental, Buenos Aires, Argentina | ||
| 593 | |a CONICET-Universidad de Buenos Aires, Instituto de Investigaciones en Biociencias Agrícolas y Ambientales (INBA), Buenos Aires, Argentina | ||
| 690 | 1 | 0 | |a ASPERPENTYN |
| 690 | 1 | 0 | |a DRECHSLERA |
| 690 | 1 | 0 | |a LYSOSPHINGOLIPID |
| 690 | 1 | 0 | |a MACROSPHELIDES |
| 690 | 1 | 0 | |a SAHA |
| 690 | 1 | 0 | |a ASPERPENTYN |
| 690 | 1 | 0 | |a BENZOIC ACID DERIVATIVE |
| 690 | 1 | 0 | |a CHROMAN DERIVATIVE |
| 690 | 1 | 0 | |a HEXOSYLPHYTOSPHYNGOSINE |
| 690 | 1 | 0 | |a HISTONE DEACETYLASE INHIBITOR |
| 690 | 1 | 0 | |a MACROSPHELIDE |
| 690 | 1 | 0 | |a NATURAL PRODUCT |
| 690 | 1 | 0 | |a PIPERAZINEDIONE |
| 690 | 1 | 0 | |a PRENYLHYDROXYBENZOIC ACID |
| 690 | 1 | 0 | |a PRENYLQUINOID |
| 690 | 1 | 0 | |a SPHINGOLIPID |
| 690 | 1 | 0 | |a SPHINGOSINE DERIVATIVE |
| 690 | 1 | 0 | |a UNCLASSIFIED DRUG |
| 690 | 1 | 0 | |a VORINOSTAT |
| 690 | 1 | 0 | |a XENOBIOTIC AGENT |
| 690 | 1 | 0 | |a ANTIFUNGAL AGENT |
| 690 | 1 | 0 | |a HISTONE DEACETYLASE |
| 690 | 1 | 0 | |a HISTONE DEACETYLASE INHIBITOR |
| 690 | 1 | 0 | |a ANTIFUNGAL ACTIVITY |
| 690 | 1 | 0 | |a ARTICLE |
| 690 | 1 | 0 | |a BIOTRANSFORMATION |
| 690 | 1 | 0 | |a DRECHSLERA |
| 690 | 1 | 0 | |a ENDOPHYTE |
| 690 | 1 | 0 | |a EPIGENETICS |
| 690 | 1 | 0 | |a FUNGUS |
| 690 | 1 | 0 | |a FUNGUS CULTURE |
| 690 | 1 | 0 | |a LIQUID CHROMATOGRAPHY-MASS SPECTROMETRY |
| 690 | 1 | 0 | |a LOLIUM |
| 690 | 1 | 0 | |a METABOLITE |
| 690 | 1 | 0 | |a MYCELIUM |
| 690 | 1 | 0 | |a NONHUMAN |
| 690 | 1 | 0 | |a PRINCIPAL COMPONENT ANALYSIS |
| 690 | 1 | 0 | |a ASCOMYCETES |
| 690 | 1 | 0 | |a CHEMISTRY |
| 690 | 1 | 0 | |a DRUG EFFECT |
| 690 | 1 | 0 | |a ENDOPHYTE |
| 690 | 1 | 0 | |a EVALUATION STUDY |
| 690 | 1 | 0 | |a ISOLATION AND PURIFICATION |
| 690 | 1 | 0 | |a LIQUID CHROMATOGRAPHY |
| 690 | 1 | 0 | |a METABOLISM |
| 690 | 1 | 0 | |a MICROBIAL SENSITIVITY TEST |
| 690 | 1 | 0 | |a PRINCIPAL COMPONENT ANALYSIS |
| 690 | 1 | 0 | |a TANDEM MASS SPECTROMETRY |
| 690 | 1 | 0 | |a ANTIFUNGAL AGENTS |
| 690 | 1 | 0 | |a ASCOMYCOTA |
| 690 | 1 | 0 | |a CHROMATOGRAPHY, LIQUID |
| 690 | 1 | 0 | |a ENDOPHYTES |
| 690 | 1 | 0 | |a HISTONE DEACETYLASE INHIBITORS |
| 690 | 1 | 0 | |a HISTONE DEACETYLASES |
| 690 | 1 | 0 | |a MICROBIAL SENSITIVITY TESTS |
| 690 | 1 | 0 | |a PRINCIPAL COMPONENT ANALYSIS |
| 690 | 1 | 0 | |a TANDEM MASS SPECTROMETRY |
| 700 | 1 | |a Gallardo, G.L. | |
| 700 | 1 | |a Rodriguez, M.A. | |
| 700 | 1 | |a Rincón, Y.A. | |
| 700 | 1 | |a Godeas, A.M. | |
| 700 | 1 | |a Cabrera, G.M. | |
| 773 | 0 | |d Wiley-VCH Verlag, 2018 |g v. 15 |k n. 8 |p Chem. Biodiversity |x 16121872 |t Chemistry and Biodiversity | |
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