Trypanosoma cruzi: Nitrogenous-base-containing phosphatides in trypomastigote forms - Isolation and chemical analysis
In trypanosomatids, little is known about the biosynthetic pathways involved in the metabolism of ethanolamine. In an attempt to clarify this point, an exhaustive analysis of the chloroform:methanol extract of T. cruzi trypomastigotes metabolically labeled with [14C]ethanolamine, in comparison with...
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Academic Press Inc.
1997
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| Acceso en línea: | Registro en Scopus DOI Handle Registro en la Biblioteca Digital |
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| LEADER | 15705caa a22013337a 4500 | ||
|---|---|---|---|
| 001 | PAPER-3195 | ||
| 003 | AR-BaUEN | ||
| 005 | 20230518203240.0 | ||
| 008 | 190411s1997 xx ||||fo|||| 00| 0 eng|d | ||
| 024 | 7 | |2 scopus |a 2-s2.0-0031238605 | |
| 024 | 7 | |2 cas |a ethanolamine, 141-43-5 | |
| 040 | |a Scopus |b spa |c AR-BaUEN |d AR-BaUEN | ||
| 030 | |a EXPAA | ||
| 100 | 1 | |a Uhrig, M.L. | |
| 245 | 1 | 0 | |a Trypanosoma cruzi: Nitrogenous-base-containing phosphatides in trypomastigote forms - Isolation and chemical analysis |
| 260 | |b Academic Press Inc. |c 1997 | ||
| 270 | 1 | 0 | |m Uhrig, M.L.; CIHIDECAR, Departamento de Quimica Organica, FCEUN, Universidad de Buenos Aires, 1428 Buenos Aires, Argentina |
| 506 | |2 openaire |e Política editorial | ||
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| 504 | |a Lederkremer, R.M., Lima, C., Ramirez, M.I., Ferguson, M.A.J., Homans, S.W., Thomas-Oates, J., Complete structure of the glycan of lipopeptidophosphoglycan from Trypanosoma cruzi epimastigotes (1991) Journal of Biological Chemistry, 266, pp. 23670-23675 | ||
| 504 | |a Lederkremer, R.M., Lima, C., Ramirez, M.I., Gonçalvez, M.F., Colli, W., Hexadecylpalmitoylglycerol or ceramide is linked to similar glycophosphoinositol anchor-like structures in Trypanosoma cruzi (1993) European Journal of Biochemistry, 218, pp. 929-936 | ||
| 504 | |a Lema, M.G., Aeberhard, E.E., Desaturation of fatty acids in Trypanosoma cruzi (1986) Lipids, 21, pp. 718-720 | ||
| 504 | |a Manku, M.S., A comparison of GLC and HPLC methods for determining fatty acid composition of evening primrose and soybean oil (1983) Journal of Chromatographic Science, 21, pp. 367-369 | ||
| 504 | |a Menon, A.K., Eppinger, M., Mayor, S., Schwarz, R.T., Phosphatidylethanolamine is the donor of the terminal phosphoethanolamine group in trypanosome glycosylphosphatidylinositols (1993) EMBO Journal, 12, pp. 1907-1914 | ||
| 504 | |a Patnaik, P.K., Field, M.C., Menon, A.K., Cross, G.A.M., Yee, M.C., Bütikofer, P., Molecular species analysis of phospholipids from Trypanosoma brucei bloodstream and procyclic forms (1993) Molecular and Biochemical Parasitology, 58, pp. 97-106 | ||
| 504 | |a Pollevick, G.D., Affranchino, J.L., Frasch, A.C.C., Sánchez, D.O., The complete sequence of a shed acute-phase antigen of Trypanosoma cruzi (1991) Molecular and Biochemical Parasitology, 47, pp. 247-250 | ||
| 504 | |a Previato, J.O., Gorin, P.A.J., Mazurek, M., Xavier, M.T., Fournet, B., Wieruszesk, J.M., Mendonça Previato, L., Primary structure of the oligosaccharide chain of lipopeptidophosphoglycan of epimastigote forms of Trypanosoma cruzi (1990) Journal of Biological Chemistry, 265, pp. 2518-2526 | ||
| 504 | |a Previato, J.O., Jones, C., Xavier, M.T., Wait, R., Travassos, L.R., Parodi, A.J., Mendonça-Previato, L., Structural characterization of the major glycosylphosphatidylinositol membrane-anchored glycoprotein from epimastigote forms of Trypanosoma cruzi Y-strain (1995) Journal of Biological Chemistry, 270, pp. 7241-7250 | ||
| 504 | |a Rifkin, M.R., Fairlamb, A.H., Transport of ethanolamine and its incorporation into the variant surface glycoprotein of bloodstream forms of Trypanosoma brucei (1985) Molecular and Biochemical Parasitology, 15, pp. 245-256 | ||
| 504 | |a Rifkin, M.R., Strobos, C.A.M., Fairlamb, A.H., Specificity of ethanolamine transport and its further metabolism in Trypanosoma brucei (1995) Journal of Biological Chemistry, 270, pp. 16160-16166 | ||
| 504 | |a Schenkman, S., Ferguson, M.A.J., Heise, N., Cardoso De Almeida, M.L., Mortara, R.A., Yoshida, N., Mucin-like glycoproteins linked to the membrane by glycosylphosphatidylinositol anchor are the major acceptors of sialic acid in a reaction catalyzed by trans-sialidase in metacyclic forms of Trypanosoma cruzi (1993) Molecular and Biochemical Parasitology, 59, pp. 293-304 | ||
| 504 | |a Shiao, Y., Vance, J.E., Evidence of an ethanolamine cycle: Differential recycling of the ethanolamine moiety of phosphatidylethanolamine derived from phosphatidylserine and ethanolamine (1995) Biochemical Journal, 310, pp. 673-679 | ||
| 504 | |a Sjursnes, B.J., Anthonsen, T., Acyl migration in 1,2-dibutyrin dependence on solvent and water activity (1994) Biocatalysis, 9, pp. 285-297 | ||
| 504 | |a Timm, S.L., Pereira-Netto, A.D., Oliveira, M.M., Fatty acids of Trypanosoma cruzi (1982) Comparative Biochemical Physiology B, 71, pp. 397-402 | ||
| 504 | |a Uhrig, M.L., Couto, A.S., Colli, W., Lederkremer, R.M., Characterization of inositolphospholipids in Trypanosoma cruzi trypomastigote forms (1996) Biochimica et Biophysica Acta, 1300, pp. 233-239 | ||
| 504 | |a Vance, D.E., Phosphatidylcholine metabolism: Masochistic enzymology, metabolic regulation and lipoprotein assembly (1990) Biochemistry of Cell Biology, 68, pp. 1151-1165 | ||
| 504 | |a Voelker, D.R., Phosphatidylserine functions as the major precursor of phosphatidylethanolamine in culture BHK-21 cells (1984) Proceedings of the National Academy of Sciences of the United States of America, 81, pp. 2669-2673 | ||
| 504 | |a Weiss, S.B., Smith, S.W., Kennedy, E.P., The enzymatic formation of lecithin from cytidine diphosphate choline and D-1,2-diglyceride (1958) Journal of Biological Chemistry, 231, pp. 53-64 | ||
| 520 | 3 | |a In trypanosomatids, little is known about the biosynthetic pathways involved in the metabolism of ethanolamine. In an attempt to clarify this point, an exhaustive analysis of the chloroform:methanol extract of T. cruzi trypomastigotes metabolically labeled with [14C]ethanolamine, in comparison with the lipids from [13H]palmitic acid-incorporated parasites, was performed. In both cases, phosphatidylethanolamine and lysophosphatidylethanolamine were detected, while phosphatidylcholine and lysophosphatidylcholine were only labeled with the fatty acid precursor. However, dimethylphosphatidylethanolamine was isolated from parasites labeled with the base precursor, indicating the ability of trypanosomes to methylate phosphatidylethanolamine to dimethylphosphatidylethanolamine. Fatty acids of the labeled phospholipids were analyzed by reverse-phase thin-layer chromatography and fluorography. Interestingly, phospholipids from the trypomastigote stage show palmitic acid (C16:0) and stearic acid (C18:0) as the only labeled components. The same saturated fatty acids were found free and as components of the radioactive triglycerides. No unsaturated fatty acids were detected, in accordance with the results obtained with inositolphospholipids. Conversely, when the fatty acids of phospholipids purified from nonlabeled parasites were analyzed by gas-liquid chromatography and gas-liquid chromatography-mass spectrometry, C18:1 was also detected. A striking finding was the presence of a considerable amount of free lignoceric acid (C24:0). Also, the C24:0 fatty acid was identified in the triglyceride fraction and as a component of phosphatidylcholine. The limited capacity of trypomastigote forms to elongate fatty acids was determined. In contrast with the results reported for other noninfective forms of the parasite, the absence of unsaturated fatty acids due to a low activity of desaturases was observed. |l eng | |
| 536 | |a Detalles de la financiación: Fundação de Amparo à Pesquisa do Estado de São Paulo, 95/ 4562-3 | ||
| 536 | |a Detalles de la financiación: Universidad de Buenos Aires | ||
| 536 | |a Detalles de la financiación: Conselho Nacional de Desenvolvimento Científico e Tecnológico, CNPq | ||
| 536 | |a Detalles de la financiación: Consejo Nacional de Investigaciones Científicas y Técnicas | ||
| 536 | |a Detalles de la financiación: Fundação de Amparo à Pesquisa do Estado de São Paulo | ||
| 536 | |a Detalles de la financiación: This investigation received financial support from UNDP/World Bank/World Health Organization Special Programme for Research and Training in Tropical Diseases, Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), and Universidad de Buenos Aires to R. M. de Lederkremer; Fundación Antorchas/Vitae to R. M. de Lederkremer and W. Colli; and Fundac¸ão de Amparo à Pesquisa do Estado de São Paulo (FAPESP Temático, 95/ 4562-3) and Conselho Nacional do Desenvolvimento Cien- tífico e Tecnológico (CNPq62.0251/94.8 PADCT II) to W. Colli and M. J. M. Alves. R.M.L. and A.S.C. are research members of CONICET. | ||
| 593 | |a Depto. de Quimica Orgánica, Fac. de Ciencias Exactas y Naturales, Universidad de Buenos Aires, 1428, Buenos Aires, Argentina | ||
| 593 | |a Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, São Paulo, Brazil | ||
| 690 | 1 | 0 | |a DAG, DIACYLGLYCEROL |
| 690 | 1 | 0 | |a DME, DULBECCO'S MODIFIED EAGLE'S MEDIUM |
| 690 | 1 | 0 | |a DMPE, DIMETHYLPHOSPHATIDYLETHANOLAMINE |
| 690 | 1 | 0 | |a FCS, FETAL CALF SERUM |
| 690 | 1 | 0 | |a GLC, GAS-LIQUID CHROMATOGRAPHY |
| 690 | 1 | 0 | |a GLC-MS, GAS- LIQUID CHROMATOGRAPHY-MASS SPECTROMETRY |
| 690 | 1 | 0 | |a GPI, GLYCOSYLPHOSPHATIDYLINOSITOL |
| 690 | 1 | 0 | |a LPC, LYSOPHOSPHATIDYLCHOLINE |
| 690 | 1 | 0 | |a LPE, LYSOPHOSPHATIDYLETHANOLAMINE |
| 690 | 1 | 0 | |a MMPE, MONOMETHYLPHOSPHATIDYLETHANOLAMINE |
| 690 | 1 | 0 | |a PC, PHOSPHATIDYLCHOLINE |
| 690 | 1 | 0 | |a PE, PHOSPHATIDYLETHANOLAMINE |
| 690 | 1 | 0 | |a PLA2, PHOSPHOLIPASE A2 |
| 690 | 1 | 0 | |a PLC, PHOSPHOLIPASE C |
| 690 | 1 | 0 | |a RP-TLC, REVERSE- PHASE THIN-LAYER CHROMATOGRAPHY |
| 690 | 1 | 0 | |a SAPA |
| 690 | 1 | 0 | |a SHED ACUTE-PHASE ANTIGEN |
| 690 | 1 | 0 | |a TAG, TRIACYLGLYCEROL |
| 690 | 1 | 0 | |a TLC, THIN-LAYER CHROMATOGRAPHY |
| 690 | 1 | 0 | |a TRYPANOSOMA CRUZI |
| 690 | 1 | 0 | |a TRYPOMASTIGOTE |
| 690 | 1 | 0 | |a ZWITTERIONIC PHOSPHOLIPIDS |
| 690 | 1 | 0 | |a ETHANOLAMINE |
| 690 | 1 | 0 | |a ARTICLE |
| 690 | 1 | 0 | |a CHEMICAL ANALYSIS |
| 690 | 1 | 0 | |a GAS LIQUID CHROMATOGRAPHY |
| 690 | 1 | 0 | |a HOST PARASITE INTERACTION |
| 690 | 1 | 0 | |a NONHUMAN |
| 690 | 1 | 0 | |a PRIORITY JOURNAL |
| 690 | 1 | 0 | |a TRYPANOSOMA CRUZI |
| 690 | 1 | 0 | |a TRYPOMASTIGOTE |
| 690 | 1 | 0 | |a CICONIIFORMES |
| 690 | 1 | 0 | |a TRYPANOSOMA |
| 690 | 1 | 0 | |a TRYPANOSOMA CRUZI |
| 690 | 1 | 0 | |a TRYPANOSOMATIDAE |
| 700 | 1 | |a Couto, A.S. | |
| 700 | 1 | |a Alves, M.J.M. | |
| 700 | 1 | |a Colli, W. | |
| 700 | 1 | |a De Lederkremer, R.M. | |
| 773 | 0 | |d Academic Press Inc., 1997 |g v. 87 |h pp. 8-19 |k n. 1 |p EXP. PARASITOL. |x 00144894 |w (AR-BaUEN)CENRE-4717 |t Experimental Parasitology | |
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| 856 | 4 | 0 | |u https://doi.org/10.1006/expr.1997.4181 |y DOI |
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| 999 | |c 64148 | ||