Protein measurement with neocuproine reactive
A new one-step method for protein measurement based in the copper-neocuproine complex formation is described. A linear response was obtained for different proteins in the range of 0.5 to 40 μg/ml with a sensitivity of 0.5 μg protein/ml. © 1991 Science & Technology Letters.
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| Autor principal: | |
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| Otros Autores: | , |
| Formato: | Capítulo de libro |
| Lenguaje: | Inglés |
| Publicado: |
Kluwer Academic Publishers
1991
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| Acceso en línea: | Registro en Scopus DOI Handle Registro en la Biblioteca Digital |
| Aporte de: | Registro referencial: Solicitar el recurso aquí |
| Sumario: | A new one-step method for protein measurement based in the copper-neocuproine complex formation is described. A linear response was obtained for different proteins in the range of 0.5 to 40 μg/ml with a sensitivity of 0.5 μg protein/ml. © 1991 Science & Technology Letters. |
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| Bibliografía: | Bradford, M.M., (1976) Anal. Biochem., 72, pp. 248-254 Dygert, S., Li, L.H., Florida, D., Thoma, J.A., (1965) Anal. Biochem., 13, pp. 367-374 Kirschenbaum, D.M., (1970) Handbook of Biochemistry, pp. 71-98. , H., Sober, Chemical Rubber Co., Cleveland Lowry, O.H., Rosembrough, N.J., Lewis Farr, A., Randall, R.J., (1951) J. Biol. Chem., 193, pp. 265-275 Mc Guire, J., Taylor, P., Greene, L.A., (1977) Anal. Biochem., 83, pp. 75-81 Pierce, J., Suelter, C.H., (1977) Anal. Biochem., 81, pp. 478-480 Sedmak, J.J., Grossberg, S.E., (1977) Anal. Biochem., 79, pp. 544-552 Smith, G.F., McCurdy, W.H., (1952) Anal. Chem., 24, pp. 371-373 Smith, P.K., Krohn, R.I., Hermanson, G.T., Mallia, A.K., Gartner, F.H., Provenzano, M.D., Fujimoto, E.K., Klenk, D.C., (1985) Anal. Biochem., 150, pp. 76-85 Van Kley, H., Hale, S.M., (1977) Anal. Biochem., 81, pp. 485-487 |
| ISSN: | 0951208X |
| DOI: | 10.1007/BF00155488 |