Heat-induced expression of a molecular chaperone decreases by selecting for long-lived individuals

The 70 kDa heat-shock protein (Hsp70) exhibits a broad range of chaperone functions that respond both to internal and external stresses, and its heat-induced expression both declines with age and reduces age-specific mortality rates. Here we test for changes in both longevity and the level of Hsp70-...

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Autor principal: Norry, F.M
Otros Autores: Loeschcke, V.
Formato: Capítulo de libro
Lenguaje:Inglés
Publicado: Elsevier Inc. 2003
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100 1 |a Norry, F.M. 
245 1 0 |a Heat-induced expression of a molecular chaperone decreases by selecting for long-lived individuals 
260 |b Elsevier Inc.  |c 2003 
270 1 0 |m Loeschcke, V.; Depto. de Ecologia, Genet./Evol., Fac. de Ciencias Exactas y Naturales, Universidad de Buenos Aires, (1428) Buenos Aires, Argentina; email: volker.loeschcke@biology.au.dk 
506 |2 openaire  |e Política editorial 
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520 3 |a The 70 kDa heat-shock protein (Hsp70) exhibits a broad range of chaperone functions that respond both to internal and external stresses, and its heat-induced expression both declines with age and reduces age-specific mortality rates. Here we test for changes in both longevity and the level of Hsp70-induced expression as correlated responses to selection on both heat-stress resistance and longevity in D. melanogaster. Three replicated H lines were heat-stress selected and compared to their respective non-selected controls (C lines) in the 25th generation of heat-stress selection. The direct response in heat-stress resistance was 56% in males and 38% in females. Heat-stress selection improved longevity in males at normal temperatures. All lines were subsequently subjected to one generation of truncation selection on longevity (selection intensity, i=1.28 for H lines and 1.36 for C lines). The heat-induced Hsp70 expression seems to increase very weakly by heat-stress selection but shows a dramatic and persistent decline by selecting for long-lived flies. A mechanism of longevity selection, involving changes in Hsp70 regulation, is suggested. © 2003 Elsevier Science Inc. All rights reserved.  |l eng 
536 |a Detalles de la financiación: Natural Sciences and Engineering Research Council of Canada 
536 |a Detalles de la financiación: Consejo Nacional de Investigaciones Científicas y Técnicas 
536 |a Detalles de la financiación: We thank Trine Gammelgaard for technical assistance, Stuart (J.S.F). Barker, Robert A. Krebs and Miriam Hercus for helpful comments on the manuscript. The Institute for Advanced Study and the Center for Environmental Stress and Adaptation Research at La Trobe University are greatly acknowledged for their hospitality to VL. This work was supported by the Carlsberg Foundation and the Danish Natural Sciences Research Council, which partially supported F.M.N.'s stay in Denmark. F.M.N.'s stay was also greatly supported by Consejo Nacional de Investigaciones Cientı́ficas y Técnicas (Argentina). 
593 |a Department of Ecology and Genetics, University of Aarhus, Bldg. 540, Ny Munkegade, DK-8000 Aarhus C, Denmark 
593 |a Depto. de Ecologia, Genet./Evol., Fac. de Ciencias Exactas y Naturales, Universidad de Buenos Aires, (1428) Buenos Aires, Argentina 
690 1 0 |a DROSOPHILA 
690 1 0 |a HEAT-STRESS RESISTANCE 
690 1 0 |a HSP70 
690 1 0 |a LONGEVITY 
690 1 0 |a TRUNCATION SELECTION 
690 1 0 |a CHAPERONE 
690 1 0 |a HEAT SHOCK PROTEIN 70 
690 1 0 |a AGING 
690 1 0 |a ARTICLE 
690 1 0 |a CONTROLLED STUDY 
690 1 0 |a DROSOPHILA MELANOGASTER 
690 1 0 |a FEMALE 
690 1 0 |a HEAT STRESS 
690 1 0 |a HERITABILITY 
690 1 0 |a LIFESPAN 
690 1 0 |a LONGEVITY 
690 1 0 |a MALE 
690 1 0 |a NONHUMAN 
690 1 0 |a PRIORITY JOURNAL 
690 1 0 |a PROTEIN EXPRESSION 
690 1 0 |a PROTEIN FUNCTION 
690 1 0 |a REGULATORY MECHANISM 
690 1 0 |a TEMPERATURE SENSITIVITY 
690 1 0 |a MELANOGASTER 
700 1 |a Loeschcke, V. 
773 0 |d Elsevier Inc., 2003  |g v. 38  |h pp. 673-681  |k n. 6  |p Exp. Gerontol.  |x 05315565  |t Experimental Gerontology 
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