Sequencing of the bicistronic genome segments S7 and S9 of Mal de Río Cuarto virus (Fijivirus, Reoviridae) completes the genome of this virus

The nucleotide sequences of genomic segments S7 and S9 of Mal de Río Cuarto virus (MRCV, Fijivirus group II) have been determined, thus completing the entire genome sequence of the virus. These segments showed a non-overlapping bicistronic structure, as in other members of the genus. MRCV S7 ORF-1 h...

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Autor principal: Guzmán, F.A
Otros Autores: Distéfano, A.J, Arneodo, J.D, Hopp, H.E, Lenardon, S.L, Del Vas, M., Conci, L.R
Formato: Capítulo de libro
Lenguaje:Inglés
Publicado: 2007
Acceso en línea:Registro en Scopus
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024 7 |2 Molecular Sequence Numbers  |a GENBANK: AB011024, AB011027, AF050086, AJ297427, AJ297430, AY789927, AY923115, D14691, D49700, DQ023312, L76561, X55701; 
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100 1 |a Guzmán, F.A. 
245 1 0 |a Sequencing of the bicistronic genome segments S7 and S9 of Mal de Río Cuarto virus (Fijivirus, Reoviridae) completes the genome of this virus 
260 |c 2007 
270 1 0 |m Guzmán, F.A.; Instituto de Fitopatología y Fisiología Vegetal, CICVyA, INTA, Córdoba, Argentina 
506 |2 openaire  |e Política editorial 
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520 3 |a The nucleotide sequences of genomic segments S7 and S9 of Mal de Río Cuarto virus (MRCV, Fijivirus group II) have been determined, thus completing the entire genome sequence of the virus. These segments showed a non-overlapping bicistronic structure, as in other members of the genus. MRCV S7 ORF-1 had a length of 1086 bp and encoded a 41.5 kDa putative polypeptide, whereas MRCV S7 ORF-2 had a length of 930 bp and encoded a 36.8 kDa putative polypeptide. Proteins of 39 and 20.5 kDa were predicted for the 1014 bp long MRCV S9 ORF-1 and the 537 bp long MRCV S9 ORF-2, respectively. The terminal 5′ and 3′ sequences of both segments were 5′AAGUUUUU3′ and 5′CAGCUnnnGUC3′, respectively. Specific imperfect inverted repeats of each segment were identified. Comparison of the predicted proteins with those of related virus genome segments counterparts in maize rough dwarf virus (MRDV) and rice black streaked dwarf virus (RBSDV), showed 64.5-44.3% identities. These values are lower than those resulting from comparisons between MRDV and RBSDV. The topology of the trees obtained using the complete nucleotide and amino acid sequences of MRCV S7 and MRCV S9 was consistent with the analysis of the other MRCV segments previously published. © 2006 Springer-Verlag.  |l eng 
593 |a Instituto de Fitopatología y Fisiología Vegetal, CICVyA, INTA, Córdoba, Argentina 
593 |a Instituto de Biotecnología, CICVyA, INTA, Castelar, Buenos Aires, Argentina 
593 |a Consejo Nacional de Investigaciones Científicas y Técnicas, CONICET, Argentina 
593 |a Unidad Integrada CNIA, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Argentina 
593 |a Instituto de Fitopatología y Fisiología Vegetal, INTA-IFFIVE, Camino 60 cuadras Km. 51/2, (X5020ICA), Córdoba, Argentina 
690 1 0 |a 3' UNTRANSLATED REGION 
690 1 0 |a 5' UNTRANSLATED REGION 
690 1 0 |a ARTICLE 
690 1 0 |a BIOLOGY 
690 1 0 |a CLASSIFICATION 
690 1 0 |a GENETICS 
690 1 0 |a MOLECULAR CLONING 
690 1 0 |a NUCLEOTIDE SEQUENCE 
690 1 0 |a PHYLOGENY 
690 1 0 |a REOVIRUS 
690 1 0 |a SEQUENCE HOMOLOGY 
690 1 0 |a VIRUS GENOME 
690 1 0 |a 3' UNTRANSLATED REGIONS 
690 1 0 |a 5' UNTRANSLATED REGIONS 
690 1 0 |a BASE SEQUENCE 
690 1 0 |a CLONING, MOLECULAR 
690 1 0 |a COMPUTATIONAL BIOLOGY 
690 1 0 |a GENOME, VIRAL 
690 1 0 |a PHYLOGENY 
690 1 0 |a REOVIRIDAE 
690 1 0 |a SEQUENCE HOMOLOGY, NUCLEIC ACID 
690 1 0 |a FIJIVIRUS 
690 1 0 |a MAIZE ROUGH DWARF VIRUS 
690 1 0 |a MAL DE RIO CUARTO VIRUS 
690 1 0 |a ORTHOREOVIRUS 
690 1 0 |a REOVIRIDAE 
690 1 0 |a RICE BLACK STREAKED DWARF VIRUS 
690 1 0 |a ZEA MAYS 
700 1 |a Distéfano, A.J. 
700 1 |a Arneodo, J.D. 
700 1 |a Hopp, H.E. 
700 1 |a Lenardon, S.L. 
700 1 |a Del Vas, M. 
700 1 |a Conci, L.R. 
773 0 |d 2007  |g v. 152  |h pp. 565-573  |k n. 3  |p Arch. Virol.  |x 03048608  |t Archives of Virology 
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