Redox driven metabolic tuning: Carbon source and aeration affect synthesis of poly(3-hydroxybutyrate) in Escherichia coli
Growth and polymer synthesis were studied in a recombinant E. coli strain carrying phaBAC and phaP of Azotobacter sp. strain FA8 using different carbon sources and oxygen availability conditions. The results obtained with glucose or glycerol were completely different, demonstrating that the metaboli...
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| Formato: | Capítulo de libro |
| Lenguaje: | Inglés |
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2010
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| Acceso en línea: | Registro en Scopus Handle Registro en la Biblioteca Digital |
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| LEADER | 09834caa a22009617a 4500 | ||
|---|---|---|---|
| 001 | PAPER-7443 | ||
| 003 | AR-BaUEN | ||
| 005 | 20230518203711.0 | ||
| 008 | 190411s2010 xx ||||fo|||| 00| 0 eng|d | ||
| 024 | 7 | |2 scopus |a 2-s2.0-79960294854 | |
| 024 | 7 | |2 cas |a glycerol, 56-81-5; poly(3 hydroxybutyric acid), 26063-00-3; Carbon, 7440-44-0; Glucose, 50-99-7; Glycerol, 56-81-5; Hydroxybutyrates; Oxygen, 7782-44-7; Polyesters; poly-beta-hydroxybutyrate, 26063-00-3 | |
| 040 | |a Scopus |b spa |c AR-BaUEN |d AR-BaUEN | ||
| 100 | 1 | |a Nikel, P.I. | |
| 245 | 1 | 0 | |a Redox driven metabolic tuning: Carbon source and aeration affect synthesis of poly(3-hydroxybutyrate) in Escherichia coli |
| 260 | |c 2010 | ||
| 270 | 1 | 0 | |m Pettinari, M. J.; Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad Nacional de Buenos Aires, Buenos Aires, Argentina; email: jul@qb.fcen.uba.ar |
| 506 | |2 openaire |e Política editorial | ||
| 504 | |a Dawes, E.A., Senior, P.J., The role and regulation of energy reserve polymers in micro-organisms (1973) Adv Microb Physiol, 10, pp. 135-266 | ||
| 504 | |a Madison, L.L., Huisman, G.W., Metabolic engineering of poly(3-hydroxyalkanoates): From dna to plastic (1999) Microbiol Mol Biol Rev, 63, pp. 21-53 | ||
| 504 | |a López, N.I., Floccari, M.E., Steinbüchel, A., García, A.F., Méndez, B.S., Effect of poly(3-hydroxybutyrate) (phb) content on the starvation-survival of bacteria in natural waters (1995) FEMS Microbiol Ecol, 16, pp. 95-112 | ||
| 504 | |a Ruiz, J.A., Fernández, R.O., Nikel, P.I., Méndez, B.S., Pettinari, M.J., Dye (arc) mutants: Insights into an unexplained phenotype and its suppression by the synthesis of poly(3-hydroxybutyrate) in escherichia coli recombinants (2006) FEMS Microbiol Lett, 258, pp. 55-60 | ||
| 504 | |a Taidi, B., Anderson, A.J., Dawes, E.A., Byrom, D., Effect of carbon source and concentration on the molecular mass of poly(3-hydroxybutyrate) produced by methylobacterium extorquens and alcaligenes eutrophus (1994) Appl Microbiol Biotechnol, 40, pp. 786-790 | ||
| 504 | |a Shiloach, J., Fass, R., Growing, E., Coli to high cell density-A historical perspective on method development (2005) Biotechnol Adv, 23, pp. 345-357 | ||
| 504 | |a Nikel, P.I., de Almeida, A., Melillo, E.C., Galvagno, M.A., Pettinari, M.J., New recombinant escherichia coli strain tailored for the production of poly(3-hydroxybutyrate) from agroindustrial by-products (2006) Appl Environ Microbiol, 72, pp. 3949-3954 | ||
| 504 | |a Solaiman, D.K., Ashby, R.D., Foglia, T.A., Marmer, W.N., Conversion of agricultural feedstock and coproducts into poly(hydroxyalkanoates) (2006) Appl Microbiol Biotechnol, 71, pp. 783-789 | ||
| 504 | |a de Almeida, A., Nikel, P.I., Giordano, A.M., Pettinari, M.J., Effects of granule-associated protein phap on glycerol-dependent growth and polymer production in poly(3-hydroxybutyrate)-producing escherichia coli (2007) Appl Environ Microbiol, 73, pp. 7912-7916 | ||
| 504 | |a Madden, L.A., Anderson, A.J., Shah, D.T., Asrar, J., Chain termination in polyhydroxyalkanoate synthesis: Involvement of exogenous hydroxy-compounds as chain transfer agents (1999) Internatl J Biol Macromol, 25, pp. 43-53 | ||
| 504 | |a Agus, J., Kahar, P., Abe, H., Doi, Y., Tsuge, T., Molecular weight characterization of poly[(r)-3-hydroxybutyrate] synthesized by genetically engineered strains of escherichia coli (2006) Polym Degrad Stab, 91, pp. 1138-1146 | ||
| 504 | |a Choi, J.I., Lee, S.Y., High level production of supra molecular weight poly(3-hydroxybutyrate) by metabolically engineered escherichia coli (2004) Biotechnol Bioprocess Eng, 9, pp. 196-200 | ||
| 504 | |a Ahn, W.S., Park, S.J., Lee, S.Y., Production of poly(3- hydroxybutyrate) by fed-batch culture of recombinant escherichia coli with a highly concentrated whey solution (2000) Appl Environ Microbiol, 66, pp. 3624-3627 | ||
| 504 | |a Lee, S.Y., Choi, J., Wong, H.H., Recent advances in polyhydroxyalkanoate production by bacterial fermentation: Mini-review (1999) Internatl J Biol Macromol, 25, pp. 31-36 | ||
| 504 | |a Nikel, P.I., Pettinari, M.J., Galvagno, M.A., Méndez, B.S., Poly(3-hydroxybutyrate) synthesis by recombinant escherichia coli arca mutants in microaerobiosis (2006) Appl Environ Microbiol, 72, pp. 2614-2620 | ||
| 504 | |a Nikel, P.I., Pettinari, M.J., Galvagno, M.A., Méndez, B.S., Poly(3-hydroxybutyrate) synthesis from glycerol by a recombinant escherichia coli arca mutant in fed-batch microaerobic cultures (2008) Appl Microbiol Biotechnol, 77, pp. 1337-1343 | ||
| 504 | |a Nikel, P.I., de Almeida, A., Pettinari, M.J., Méndez, B.S., The legacy of hfrh: Mutations in the two-component system crebc are responsible for the unusual phenotype of an escherichia coli arca mutant (2008) J Bacteriol, 190, pp. 3404-3407 | ||
| 504 | |a de Almeida, A., Giordano, A.M., Nikel, P.I., Pettinari, M.J., Effects of aeration on the synthesis of poly(3-hydroxybutyrate) from glycerol and glucose in recombinant escherichia coli (2010) Appl Environ Microbiol, 76, pp. 2036-2040 | ||
| 504 | |a Shalel-Levanon, S., San, K.Y., Bennett, G.N., Effect of oxygen on the escherichia coli arca and fnr regulation systems and metabolic responses (2005) Biotechnol Bioeng, 89, pp. 556-564 | ||
| 504 | |a Clark, D.P., The fermentation pathways of escherichia coli (1989) FEMS Microbiol Rev, 5, pp. 223-234 | ||
| 504 | |a San, K.Y., Bennett, G.N., Berríos-Rivera, S.J., Vidali, R.V., Yang, Y.T., Horton, R.E., Metabolic engineering through cofactor manipulation and its effect on metabolic flux redistribution in escherichia coli (2002) Metab Eng, 4, pp. 182-192 | ||
| 504 | |a Nikel, P.I., Pettinari, M.J., Ramirez, M.C., Galvagno, M.A., Méndez, B.S., Escherichia coli arca mutants: Metabolic profile characterization of microaerobic cultures using glycerol as a carbon source (2008) J Mol Microbiol Biotechnol, 15, pp. 48-54 | ||
| 520 | 3 | |a Growth and polymer synthesis were studied in a recombinant E. coli strain carrying phaBAC and phaP of Azotobacter sp. strain FA8 using different carbon sources and oxygen availability conditions. The results obtained with glucose or glycerol were completely different, demonstrating that the metabolic routes leading to the synthesis of the polymer when using glycerol do not respond to environmental conditions such as oxygen availability in the same way as they do when other substrates, such as glucose, are used. When cells were grown in a bioreactor using glucose the amount of polymer accumulated at low aeration was reduced by half when compared to high aeration, while glycerol cultures produced at low aeration almost twice the amount of polymer synthesized at the higher aeration condition. The synthesis of other metabolic products, such as ethanol, lactate, formate and acetate, were also affected by both the carbon source used and aeration conditions. In glucose cultures, lactate and formate production increased in low agitation compared to high agitation, while poly(3-hydroxybutyrate) synthesis decreased. In glycerol cultures, the amount of acids produced also increased when agitation was lowered, but carbon flow was mostly redirected towards ethanol and poly(3-hydroxybutyrate). These results indicated that carbon partitioning differed depending on both carbon source and oxygen availability, and that aeration conditions had different effects on the synthesis of the polymer and other metabolic products when glucose or glycerol were used. © 2010 Landes Bioscience. |l eng | |
| 536 | |a Detalles de la financiación: Universidad de Buenos Aires, X173 | ||
| 536 | |a Detalles de la financiación: Financial funding by Universidad de Buenos Aires (Project X173) is grate fully acknowledged. P.I.N. and M.J.P. are researchers from Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET, Argentina). A.D.A. holds a post-doctoral fellowship from CONICET. | ||
| 593 | |a Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad Nacional de Buenos Aires, Buenos Aires, Argentina | ||
| 593 | |a Instituto de Investigaciones Biotecnológicas, Universidad Nacional de San Martín, Buenos Aires, Argentina | ||
| 690 | 1 | 0 | |a AERATION |
| 690 | 1 | 0 | |a CARBON PARTITIONING |
| 690 | 1 | 0 | |a ESCHERICHIA COLI |
| 690 | 1 | 0 | |a GLUCOSE |
| 690 | 1 | 0 | |a GLYCEROL |
| 690 | 1 | 0 | |a OXYGEN AVAILABILITY |
| 690 | 1 | 0 | |a POLY(3-HYDROXYBUTYRATE) |
| 690 | 1 | 0 | |a GLYCEROL |
| 690 | 1 | 0 | |a POLY(3 HYDROXYBUTYRIC ACID) |
| 690 | 1 | 0 | |a AERATION |
| 690 | 1 | 0 | |a ARTICLE |
| 690 | 1 | 0 | |a BACTERIAL GROWTH |
| 690 | 1 | 0 | |a BACTERIAL METABOLISM |
| 690 | 1 | 0 | |a BACTERIUM CULTURE |
| 690 | 1 | 0 | |a CARBON SOURCE |
| 690 | 1 | 0 | |a ESCHERICHIA COLI |
| 690 | 1 | 0 | |a NONHUMAN |
| 690 | 1 | 0 | |a OXIDATION REDUCTION REACTION |
| 690 | 1 | 0 | |a SYNTHESIS |
| 690 | 1 | 0 | |a ESCHERICHIA COLI |
| 690 | 1 | 0 | |a GLUCOSE |
| 690 | 1 | 0 | |a GLYCEROL |
| 690 | 1 | 0 | |a HYDROXYBUTYRATES |
| 690 | 1 | 0 | |a OXIDATION-REDUCTION |
| 690 | 1 | 0 | |a OXYGEN |
| 690 | 1 | 0 | |a POLYESTERS |
| 690 | 1 | 0 | |a AZOTOBACTER |
| 690 | 1 | 0 | |a ESCHERICHIA COLI |
| 650 | 1 | 7 | |2 spines |a CARBON |
| 700 | 1 | |a de Almeida, A. | |
| 700 | 1 | |a Giordano, A.M. | |
| 700 | 1 | |a Pettinari, M.J. | |
| 773 | 0 | |d 2010 |g v. 1 |h pp. 291-295 |k n. 4 |p Bioeng. Bugs |x 19491018 |t Bioengineered Bugs | |
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| 856 | 4 | 0 | |u https://hdl.handle.net/20.500.12110/paper_19491018_v1_n4_p291_Nikel |y Handle |
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