Optical techniques provide information on various effective diffusion coefficients in the presence of traps

Mostrar otras versiones (2)

In many cell-signaling pathways information is transmitted via the diffusion of messenger molecules. In most cases, messengers react with other substances and diffuse at the same time. Effective diffusion coefficients may be introduced to characterize the net transport rate that results from the com...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autor principal: Sigaut, Lorena
Otros Autores: Ponce, M.L, Colman-Lerner, A., Dawson, S.P
Formato: Capítulo de libro
Lenguaje:Inglés
Publicado: American Physical Society 2010
Acceso en línea:Registro en Scopus
DOI
Handle
Registro en la Biblioteca Digital
Aporte de:Registro referencial: Solicitar el recurso aquí
Biblioteca Central Dr. Luis F. Leloir (FCEN) de Universidad de Buenos Aires
LEADER 07320caa a22008537a 4500
001 PAPER-7516
003 AR-BaUEN
005 20251028095804.0
008 190411s2010 xx ||||fo|||| 00| 0 eng|d
024 7 |2 scopus  |a 2-s2.0-78651376841 
030 |a PLEEE 
040 |a Scopus  |b spa  |c AR-BaUEN  |d AR-BaUEN 
100 1 |a Sigaut, Lorena 
245 1 0 |a Optical techniques provide information on various effective diffusion coefficients in the presence of traps 
260 |b American Physical Society  |c 2010 
270 1 0 |m Sigaut, L.; Departamento de Física, FCEN-UBA, Ciudad Universitaria, Pabellón I, (1428) Buenos Aires, Argentina; email: lorena@df.uba.ar 
504 |a Alberts, B., Johnson, A., Lewis, J., Raff, M., Roberts, K., Walter, P., (2007) Molecular Biology of the Cell, , 5th ed. (Garland Science, Oxford, UK 
504 |a Ellis, R.J., (2001) Curr. Opin. Struct. Biol., 11, p. 114. , 10.1016/S0959-440X(00)00172-X 
504 |a Pando, B., Ponce Dawson, S., Mak, D.D., Pearson, J.E., (2006) Proc. Natl. Acad. Sci. U.S.A., 103, p. 5338. , 10.1073/pnas.0509576103 
504 |a Axelrod, D., Koppel, D.E., Schlessinger, J., Elson, E., Webb, W.W., (1976) Biophys. J., 16, p. 1055. , 10.1016/S0006-3495(76)85755-4 
504 |a Jacobson, K., Wojcieszyn, J., (1984) Proc. Natl. Acad. Sci. U.S.A., 81, p. 6747. , 10.1073/pnas.81.21.6747 
504 |a Gribbon, P., Hardingham, T.E., (1998) Biophys. J., 75, p. 1032. , 10.1016/S0006-3495(98)77592-7 
504 |a Magde, D., Elson, E., Webb, W.W., (1972) Phys. Rev. Lett., 29, p. 705. , 10.1103/PhysRevLett.29.705 
504 |a Elson, E.L., (2001) Traffic, 2, p. 789. , 10.1034/j.1600-0854.2001.21107.x 
504 |a Kim, S.A., Schwille, P., (2003) Curr. Opin. Neurobiol., 13, p. 583. , 10.1016/j.conb.2003.09.002 
504 |a Krichevsky, O., Bonnet, G., (2002) Rep. Prog. Phys., 65, p. 251. , 10.1088/0034-4885/65/2/203 
504 |a Grünwald, D., Cardoso, M.C., Leonhardt, H., Buschmann, V., (2005) Curr. Pharm. Biotechnol., 6, p. 381. , 10.2174/138920105774370616 
504 |a Sprague, B.L., Pego, R.L., Stavreva, D.A., McNally, J.G., (2004) Biophys. J., 86, p. 3473. , 10.1529/biophysj.103.026765 
504 |a Sprague, B.L., McNally, J.G., (2005) Trends Cell Biol., 15, p. 84. , 10.1016/j.tcb.2004.12.001 
504 |a Braeckmans, K., Peeters, L., Sanders, N.N., De Smedt, S.C., Demeester, J., (2003) Biophys. J., 85, p. 2240. , 10.1016/S0006-3495(03)74649-9 
504 |a Stepanenko, O.V., Verkhusha, V.V., Kuznetsova, I.M., Uversky, V.N., Turoverov, K.K., (2008) Curr. Protein Pept. Sci., 9, p. 338. , 10.2174/138920308785132668 
504 |a Chudakov, D., Lukyanov, S., Lukyanov, K., (2005) Trends Biotechnol., 23, p. 605. , 10.1016/j.tibtech.2005.10.005 
504 |a Walling, M.A., Novak, J.A., Shepard, J.R.E., (2009) Int. J. Mol. Sci., 10, p. 441. , 10.3390/ijms10020441 
504 |a Keppler, A., Gendreizig, S., Gronemeyer, T., Pick, H., Vogel, H., Johnsson, K., (2003) Nat. Biotechnol., 21, p. 86. , 10.1038/nbt765 
504 |a Smith, C., (2007) Nat. Methods, 4, p. 755. , 10.1038/nmeth0907-755 
504 |a Bismuto, E., Gratton, E., Lamb, D.C., (2001) Biophys. J., 81, p. 3510. , 10.1016/S0006-3495(01)75982-6 
504 |a Lamb, D.C., Schenk, A., Röcker, C., Scalfi-Happ, C., Ulrich Nienhaus, G., (2000) Biophys. J., 79, p. 1129. , 10.1016/S0006-3495(00)76366-1 
504 |a Meseth, U., Wohland, T., Rigler, R., Vogel, H., (1999) Biophys. J., 76, p. 1619. , 10.1016/S0006-3495(99)77321-2 
504 |a Rein Ten Wolde, P., Tǎnase-Nicola, S., (2006) Nat. Phys., 2, p. 371. , 10.1038/nphys328 
504 |a Abu-Arish, A., Porcher, A., Czerwonka, A., Dostatni, N., Fradin, C., (2010) Biophys. J., 99, p. 33. , 10.1016/j.bpj.2010.05.031 
504 |a Gregor, T., Wieschaus, E.F., McGregor, A.P., Bialek, W., Tank, D.W., (2007) Cell, 130, p. 141. , 10.1016/j.cell.2007.05.026 
506 |2 openaire  |e Política editorial 
520 3 |a In many cell-signaling pathways information is transmitted via the diffusion of messenger molecules. In most cases, messengers react with other substances and diffuse at the same time. Effective diffusion coefficients may be introduced to characterize the net transport rate that results from the combined effect of these two processes. It was shown in that even in the simplest scenario in which one bimolecular reaction is involved, two different effective coefficients are relevant. One gives the rate at which small perturbations spread out with time while the other relates the mean square displacement of a single particle to the time elapsed. They coincide in the absence of reactions but may be very different in other cases. Optical techniques provide a relatively noninvasive means by which transport rates can be estimated. In the above mentioned paper it was discussed why, under certain conditions, fluorescence recovery after photobleaching (FRAP), a technique commonly used to estimate diffusion rates in cells, provides information on one of the two effective coefficients. In the present paper we show that, under the same conditions, another commonly used optical technique, fluorescence correlation spectroscopy (FCS), gives information on the other one. This opens up the possibility of combining experiments to obtain information that goes beyond effective transport rates. In the present paper we discuss different ways to do so. © 2010 The American Physical Society.  |l eng 
593 |a Departamento de Física, FCEN-UBA, Ciudad Universitaria, Pabellón I, (1428) Buenos Aires, Argentina 
593 |a Instituto de Fisiología, Biología Molecular y Neurociencias, Ciudad Universitaria, Pabellón II, (1428) Buenos Aires, Argentina 
690 1 0 |a CELL SIGNALING 
690 1 0 |a FLUORESCENCE 
690 1 0 |a FLUORESCENCE SPECTROSCOPY 
690 1 0 |a MOLECULAR BIOLOGY 
690 1 0 |a OPTICAL CORRELATION 
690 1 0 |a OPTICAL ENGINEERING 
690 1 0 |a PHOTOBLEACHING 
690 1 0 |a SPECTROSCOPIC ANALYSIS 
690 1 0 |a BIMOLECULAR REACTION 
690 1 0 |a EFFECTIVE DIFFUSION COEFFICIENTS 
690 1 0 |a FLUORESCENCE CORRELATION SPECTROSCOPY 
690 1 0 |a FLUORESCENCE RECOVERY AFTER PHOTOBLEACHING 
690 1 0 |a MEAN SQUARE DISPLACEMENT 
690 1 0 |a NET TRANSPORT RATE 
690 1 0 |a SIGNALING PATHWAYS 
690 1 0 |a SMALL PERTURBATIONS 
690 1 0 |a DIFFUSION 
700 1 |a Ponce, M.L. 
700 1 |a Colman-Lerner, A. 
700 1 |a Dawson, S.P. 
773 0 |d American Physical Society, 2010  |g v. 82  |k n. 5  |p Phys. Rev. E Stat. Nonlinear Soft Matter Phys.  |x 15393755  |t Physical Review E - Statistical, Nonlinear, and Soft Matter Physics 
856 4 1 |u https://www.scopus.com/inward/record.uri?eid=2-s2.0-78651376841&doi=10.1103%2fPhysRevE.82.051912&partnerID=40&md5=2785ecfb8ff97cf4883b93d2941909d3  |y Registro en Scopus 
856 4 0 |u https://doi.org/10.1103/PhysRevE.82.051912  |y DOI 
856 4 0 |u https://hdl.handle.net/20.500.12110/paper_15393755_v82_n5_p_Sigaut  |y Handle 
856 4 0 |u https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_15393755_v82_n5_p_Sigaut  |y Registro en la Biblioteca Digital 
961 |a paper_15393755_v82_n5_p_Sigaut  |b paper  |c PE 
962 |a info:eu-repo/semantics/article  |a info:ar-repo/semantics/artículo  |b info:eu-repo/semantics/publishedVersion 
963 |a VARI 
999 |c 68469 

Ejemplares similares