X-Y sample scanning stage and calibration method suitable for single-molecule detection
This paper describes the construction of a positioning device for sample scanning in the x and y directions suitable for single molecule fluorescence experiments. The mechanism uses a simple parallelogram flexure cut out of a single aluminum plate and two amplified piezoelectric actuators of the typ...
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| Formato: | Capítulo de libro |
| Lenguaje: | Inglés |
| Publicado: |
2010
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| Acceso en línea: | Registro en Scopus DOI Handle Registro en la Biblioteca Digital |
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| LEADER | 10809caa a22010817a 4500 | ||
|---|---|---|---|
| 001 | PAPER-7579 | ||
| 003 | AR-BaUEN | ||
| 005 | 20230518203720.0 | ||
| 008 | 190411s2010 xx ||||fo|||| 00| 0 eng|d | ||
| 024 | 7 | |2 scopus |a 2-s2.0-84755161619 | |
| 040 | |a Scopus |b spa |c AR-BaUEN |d AR-BaUEN | ||
| 030 | |a SABCE | ||
| 100 | 1 | |a Treegate, K. | |
| 245 | 1 | 0 | |a X-Y sample scanning stage and calibration method suitable for single-molecule detection |
| 260 | |c 2010 | ||
| 270 | 1 | 0 | |m Hodak, J. H.; Department of Physics, Faculty of Science, Mahidol University, Rama 6 Rd, Phayathai, Bangkok 10400, Thailand; email: jhodak@qi.fcen.uba.ar |
| 506 | |2 openaire |e Política editorial | ||
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| 504 | |a Xie, X.S., Trautman, J.K., Optical studies of single molecules at room temperature (1998) Annu. Rev. Phys. Chem., 49, pp. 441-480 | ||
| 504 | |a Awtar, S., Slocum, A.H., Constraint-based design of parallel kinematic XY flexure mechanisms (2007) ASME J. Mech. Design, 119, pp. 816-830 | ||
| 504 | |a Awtar, S., Slocum, A.H., Sevincer, E., Characteristics of beam-based flexure modules (2007) ASME J. Mech. Design, 119, pp. 225-639 | ||
| 504 | |a Chu, C.L., Fan, S.H., A novel long-travel piezoelectric-driven linear nanopositioning stage (2006) Precis. Eng. J. Int. Soc. Precis. Eng. Nanotechnol., 30, pp. 85-95 | ||
| 504 | |a Choi, S.B., Han, S.S., Lee, Y.S., Fine motion control of a moving stage using a piezoactuator associated with a displacement amplifier (2005) Smart Mater. Struct., 14, pp. 222-230 | ||
| 504 | |a Choi, K.-B., Kim, D.-H., Monolithic parallel linear compliant mechanism for two axes ultraprecision linear motion (2006) Rev. Sci. Instrum., 77, p. 065106 | ||
| 504 | |a Smith, A.G., David, C.B., (2006) Handbook of Single Molecule Fluorescence Spectroscopy, , Oxford University Press Oxford | ||
| 504 | |a Smith, S.T., (2000) Flexures: Elements of Elastic Mechanisms, , Gordon and Breach Science Publishers Elsevier | ||
| 504 | |a Devasia, S., Eleftheriou, E., Moheimani, S.O.R., A survey of control issues in nanopositioning (2007) IEEE Trans. Contr. Sys. Technol., 15, pp. 802-823 | ||
| 504 | |a Hodak, J.H., Downey, C.D., Fiore, J.L., Pardi, A., Nesbitt, D.J., Docking kinetics and equilibrium of a GAAA tetraloop-receptor motif probed by single-molecule FRET (2005) Proc. Natl. Acad. Sci., 102, pp. 10505-10510 | ||
| 504 | |a Xu, H.G., Ono, T., Esashi, M., Precise motion control of a nanopositioning PZT microstage using integrated capacitive displacement sensors (2006) J. Micromech. Microeng., 16, pp. 2747-2754 | ||
| 504 | |a Yeh, H.C., Ni, W.T., Pan, S.S., Real-time motion control with subnanometer heterodyne interferometry (2002) Int. J. Modern Phys. D, 11, pp. 1087-1099 | ||
| 504 | |a Yeh, H.C., Ni, W.T., Pan, S.S., Digital closed-loop nanopositioning using rectilinear flexure stage and laser interferometry (2005) Control Eng. Pract., 13, pp. 559-566 | ||
| 504 | |a Schitter, G., Stemmer, A., Fast closed loop control of piezoelectric transducers (2002) J. Vac. Sci. Technol. B, 20, pp. 350-352 | ||
| 504 | |a Schitter, G., Stark, R.W., Stemmer, A., Sensors for closed-loop piezo control: Strain gauges versus optical sensors (2002) Meas. Sci. Technol., 13, pp. 47-N48 | ||
| 504 | |a Hell, S.W., Far-field optical nanoscopy (2007) Science, 316, pp. 1153-1156 | ||
| 504 | |a Pritchard, J., Ramesh, R., Bowen, C.R., Time-temperature profiles of multi-layer actuators (2004) Sens. Actuators A: Phys., 115, pp. 140-145 | ||
| 504 | |a Jung, H., Gweon, D.-G., Creep characteristics of piezoelectric actuators (2000) Rev. Sci. Instrum., 71, pp. 1896-1900 | ||
| 520 | 3 | |a This paper describes the construction of a positioning device for sample scanning in the x and y directions suitable for single molecule fluorescence experiments. The mechanism uses a simple parallelogram flexure cut out of a single aluminum plate and two amplified piezoelectric actuators of the type used for microscope objective focus adjustment. A displacement range of 75 μm on each axis is obtained. The stage can be used to implement a sample scanning confocal microscope for single molecule spectroscopy applications using either inverted or up-right microscopes. Images with diffraction limited resolution can be obtained with this scanning stage. This is demonstrated by imaging glass beads labeled with the DY475 fluorescent dye and single rhodamine molecules. Micron sized range images of 256 × 256 pixels can be obtained with dwell times down to 0.5 ms/pixel. A novel direct calibration in which the mechanical response obtained from the line profiles for forward and reverse motion is used to account for the hysteresis of the stage. The target molecules are then located within the focus of the laser beam by using its corrected position. The performance of this scanning device and correction technique are demonstrated for the acquisition of fluorescence trajectories of individual rhodamine molecules. © 2010 Published by Elsevier B.V. |l eng | |
| 536 | |a Detalles de la financiación: Thailand Research Fund | ||
| 536 | |a Detalles de la financiación: National Science and Technology Development Agency | ||
| 536 | |a Detalles de la financiación: Thailand Graduate Institute of Science and Technology | ||
| 536 | |a Detalles de la financiación: Mahidol University | ||
| 536 | |a Detalles de la financiación: We thank Dr. Mariano Bossi at Department of NanoBiophotonik (200) Max Plank Institute for Biophysical Chemistry, for his kind gift of the fluorescent glass beads and Prof. Pedro Aramendia from The Department of Chemistry, University of Buenos Aires for giving us the rhodamine dye samples. A. Rasamessard was supported by the Thailand Research Fund through the Royal Golden Jubilee scholarship. We thank NSTDA for a TGIST scholarship supporting Ph.D. student Ketvalee Treegate. A-TEAM is kindly acknowledged for the precision wire-cut fabrication service. Appendix A | ||
| 536 | |a Detalles de la financiación: Ketvalee Treegate was born in Lopburi, Thailand, on August 25, 1984. She is receiving her Ph.D. degree in physics from The Faculty of Science, Mahidol University, Bangkok, Thailand, in 2010. Ketvalee Treegate has been working as a research assistant in the Department of Physics of Mahidol University and has been awarded a TGIST scholarship from the National Science Development Agency of Thailand. Her research is on single molecule spectroscopy and studies of iron and iron boride magnetic nanoparticles. Areefen Rasamessard was born in Yala, Thailand on July 24, 1980. He received his Ph.D. degree in physics from The Faculty of Science, Mahidol University, Bangkok, Thailand, in October, 2009. He has been working as a research assistant in the Department of Physics of Mahidol University, and also at Academia Sinica at Taiwan where he carried out fluorescence experiments on single aggregates of luminescent polymers. His research interests are in the fields of light emitting polymers. Tanakorn Osotchan was born in Pathumthani, Thailand on November 22, 1965. He obtained a Ph.D. degree in semiconductor physics from Macquarie University, New South Wales, Australia in 1995. Now, he is assistant professor at the Department of Physics, Mahidol University, Bangkok, Thailand. His research interests are organic based light emitting displays and semiconductor physics. Jose Hector Hodak was born in 1968 in Buenos Aires, Argentina. He obtained his Ph.D. in Physical Chemistry at University of Notre Dame, Indiana, USA, in 2001 studying the ultra-fast dynamics of metal nanoparticles. Dr. Hodak worked from 2001 to 2004 as a postdoctoral researcher at JILA-University of Colorado, where he carried out single molecule fluorescence experiments in RNA. He worked as researcher at the Department of Physics, Mahidol University from 2005 until 2010. He is currently an independent investigator of CONICET and assistant professor at the University of Buenos Aires. His research interests are single molecule and ultrafast spectroscopies. | ||
| 593 | |a Department of Physics, Faculty of Science, Mahidol University, Rama 6 Rd, Phayathai, Bangkok 10400, Thailand | ||
| 593 | |a INQUIMAE-Departamento de Quimica Inorganica, Analitica y Quimica Fisca, Facultad de Ciencias Exactas y Naturales, Ciudad Universitaria, AR-1428 Buenos Aires, Argentina | ||
| 690 | 1 | 0 | |a FLUORESCENCE SPECTROSCOPY |
| 690 | 1 | 0 | |a OPTICAL DETECTION |
| 690 | 1 | 0 | |a SCANNING CONFOCAL MICROSCOPY |
| 690 | 1 | 0 | |a SINGLE MOLECULE SPECTROSCOPY |
| 690 | 1 | 0 | |a ALUMINUM PLATES |
| 690 | 1 | 0 | |a CALIBRATION METHOD |
| 690 | 1 | 0 | |a CORRECTION TECHNIQUES |
| 690 | 1 | 0 | |a CUT OUT |
| 690 | 1 | 0 | |a DIFFRACTION-LIMITED RESOLUTION |
| 690 | 1 | 0 | |a DIRECT CALIBRATION |
| 690 | 1 | 0 | |a DWELL TIME |
| 690 | 1 | 0 | |a FLUORESCENT DYES |
| 690 | 1 | 0 | |a FOCUS ADJUSTMENT |
| 690 | 1 | 0 | |a IMAGING GLASS |
| 690 | 1 | 0 | |a LINE PROFILES |
| 690 | 1 | 0 | |a MECHANICAL RESPONSE |
| 690 | 1 | 0 | |a MICROSCOPE OBJECTIVE |
| 690 | 1 | 0 | |a OPTICAL DETECTION |
| 690 | 1 | 0 | |a POSITIONING DEVICES |
| 690 | 1 | 0 | |a RANGE IMAGES |
| 690 | 1 | 0 | |a SCANNING CONFOCAL MICROSCOPES |
| 690 | 1 | 0 | |a SCANNING CONFOCAL MICROSCOPY |
| 690 | 1 | 0 | |a SCANNING DEVICE |
| 690 | 1 | 0 | |a SCANNING STAGES |
| 690 | 1 | 0 | |a SINGLE MOLECULE FLUORESCENCE |
| 690 | 1 | 0 | |a SINGLE MOLECULE SPECTROSCOPY |
| 690 | 1 | 0 | |a SINGLE-MOLECULE DETECTION |
| 690 | 1 | 0 | |a TARGET MOLECULE |
| 690 | 1 | 0 | |a CALIBRATION |
| 690 | 1 | 0 | |a CONFOCAL MICROSCOPY |
| 690 | 1 | 0 | |a FLUORESCENCE |
| 690 | 1 | 0 | |a FLUORESCENCE SPECTROSCOPY |
| 690 | 1 | 0 | |a MICROSCOPES |
| 690 | 1 | 0 | |a PIEZOELECTRIC ACTUATORS |
| 690 | 1 | 0 | |a SCANNING |
| 690 | 1 | 0 | |a MOLECULES |
| 700 | 1 | |a Rasamessard, A. | |
| 700 | 1 | |a Osotchan, T. | |
| 700 | 1 | |a Hodak, J.H. | |
| 773 | 0 | |d 2010 |g v. 150 |h pp. 239-246 |k n. 1 |p Sens Actuators, B Chem |x 09254005 |w (AR-BaUEN)CENRE-6816 |t Sensors and Actuators, B: Chemical | |
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| 856 | 4 | 0 | |u https://doi.org/10.1016/j.snb.2010.07.008 |y DOI |
| 856 | 4 | 0 | |u https://hdl.handle.net/20.500.12110/paper_09254005_v150_n1_p239_Treegate |y Handle |
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