MAPK and PI3K activities are required for leptin stimulation of protein synthesis in human trophoblastic cells
Leptin, the LEP gene product, is produced in placenta where it has been found to be an important autocrine signal for trophoblastic growth during pregnancy. Thus, we have recently described the antiapoptotic and trophic effect of leptin on choriocarcinoma cell line JEG-3, stimulating DNA and protein...
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2010
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| LEADER | 20147caa a22018377a 4500 | ||
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| 001 | PAPER-7819 | ||
| 003 | AR-BaUEN | ||
| 005 | 20230518203736.0 | ||
| 008 | 190411s2010 xx ||||fo|||| 00| 0 eng|d | ||
| 024 | 7 | |2 scopus |a 2-s2.0-77953289537 | |
| 024 | 7 | |2 cas |a DNA, 9007-49-2; Janus kinase, 161384-16-3; leucine, 61-90-5, 7005-03-0; mitogen activated protein kinase, 142243-02-5; phosphatidylinositol 3 kinase, 115926-52-8; protein kinase B, 148640-14-6; tritium, 10028-17-8; 1-Phosphatidylinositol 3-Kinase, 2.7.1.137; Adaptor Proteins, Signal Transducing; EIF4EBP1 protein, human; Eukaryotic Initiation Factor-4E; Leptin; Mitogen-Activated Protein Kinase Kinases, 2.7.12.2; Phosphoproteins; Proto-Oncogene Proteins c-akt, 2.7.11.1; Ribosomal Protein S6 Kinases, 70-kDa, 2.7.11.1 | |
| 040 | |a Scopus |b spa |c AR-BaUEN |d AR-BaUEN | ||
| 030 | |a BBRCA | ||
| 100 | 1 | |a Pérez-Pérez, A. | |
| 245 | 1 | 0 | |a MAPK and PI3K activities are required for leptin stimulation of protein synthesis in human trophoblastic cells |
| 260 | |c 2010 | ||
| 270 | 1 | 0 | |m Sánchez-Margalet, V.; Department of Clinical Biochemistry, Virgen Macarena University Hospital, University of SevilleSpain; email: margalet@us.es |
| 506 | |2 openaire |e Política editorial | ||
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| 520 | 3 | |a Leptin, the LEP gene product, is produced in placenta where it has been found to be an important autocrine signal for trophoblastic growth during pregnancy. Thus, we have recently described the antiapoptotic and trophic effect of leptin on choriocarcinoma cell line JEG-3, stimulating DNA and protein synthesis. We have also demonstrated the presence of leptin receptor and leptin signaling in normal human trophoblastic cells, activating JAK-STAT, PI3K and MAPK pathways. In the present work we have employed dominant negative forms of MAPK and PKB constructs to find out the signaling pathways that specifically mediates the effect of leptin on protein synthesis. As previously shown, leptin stimulates protein synthesis as assessed by 3 H-leucine incorporation. However, both dominant negative forms of MAPK and PKB inhibited protein synthesis in JEG-3 choriocarcinoma cells. The inhibition of PKB and MAPK activity by transfection with the dominant negative kinases prevented the leptin stimulation of p70 S6K, which is known to be an important kinase in the regulation of protein synthesis. Moreover, leptin stimulation of phosphorylation of EIF4EBP1 and EIF4E, which allows the initiation of translation was also prevented by MAPK and PI3K dominant negative constructs. Therefore, these results demonstrate that both PI3K and MAPK are necessary to observe the effect of leptin signaling that mediates protein synthesis in choriocarcinoma cells JEG-3. © 2010 Elsevier Inc. All rights reserved. |l eng | |
| 536 | |a Detalles de la financiación: Instituto de Salud Carlos III, PS09/00119, ISCIII CM07/00025 | ||
| 536 | |a Detalles de la financiación: This work was supported by a Grant from the Instituto de Salud Carlos III (ISCIII PS09/00119). Antonio Pérez-Pérez is a research fellow supported by the Instituto de Salud Carlos III (Contrato Post-formación especializada, Río Hortega), ISCIII CM07/00025, Spain. | ||
| 593 | |a Department of Clinical Biochemistry, Virgen Macarena University Hospital, University of Seville, Spain | ||
| 593 | |a Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Argentina | ||
| 690 | 1 | 0 | |a LEPTIN |
| 690 | 1 | 0 | |a PROTEIN SYNTHESIS |
| 690 | 1 | 0 | |a SIGNAL TRANSDUCTION |
| 690 | 1 | 0 | |a TRANSLATION |
| 690 | 1 | 0 | |a DNA |
| 690 | 1 | 0 | |a INITIATION FACTOR 4E |
| 690 | 1 | 0 | |a INITIATION FACTOR 4E BINDING PROTEIN 1 |
| 690 | 1 | 0 | |a JANUS KINASE |
| 690 | 1 | 0 | |a LEPTIN |
| 690 | 1 | 0 | |a LEPTIN RECEPTOR |
| 690 | 1 | 0 | |a LEUCINE |
| 690 | 1 | 0 | |a MITOGEN ACTIVATED PROTEIN KINASE |
| 690 | 1 | 0 | |a PHOSPHATIDYLINOSITOL 3 KINASE |
| 690 | 1 | 0 | |a PROTEIN KINASE B |
| 690 | 1 | 0 | |a S6 KINASE |
| 690 | 1 | 0 | |a STAT PROTEIN |
| 690 | 1 | 0 | |a TRITIUM |
| 690 | 1 | 0 | |a APOPTOSIS |
| 690 | 1 | 0 | |a ARTICLE |
| 690 | 1 | 0 | |a AUTOCRINE EFFECT |
| 690 | 1 | 0 | |a CELL GROWTH |
| 690 | 1 | 0 | |a CHORIOCARCINOMA |
| 690 | 1 | 0 | |a CONTROLLED STUDY |
| 690 | 1 | 0 | |a DNA SYNTHESIS |
| 690 | 1 | 0 | |a ENZYME ACTIVATION |
| 690 | 1 | 0 | |a ENZYME ACTIVITY |
| 690 | 1 | 0 | |a ENZYME INHIBITION |
| 690 | 1 | 0 | |a GENETIC TRANSFECTION |
| 690 | 1 | 0 | |a HUMAN |
| 690 | 1 | 0 | |a HUMAN CELL |
| 690 | 1 | 0 | |a PREGNANCY |
| 690 | 1 | 0 | |a PRIORITY JOURNAL |
| 690 | 1 | 0 | |a PROTEIN PHOSPHORYLATION |
| 690 | 1 | 0 | |a PROTEIN SYNTHESIS |
| 690 | 1 | 0 | |a PROTEIN SYNTHESIS INHIBITION |
| 690 | 1 | 0 | |a PROTEIN SYNTHESIS REGULATION |
| 690 | 1 | 0 | |a SIGNAL TRANSDUCTION |
| 690 | 1 | 0 | |a TRANSLATION INITIATION |
| 690 | 1 | 0 | |a TROPHOBLAST |
| 690 | 1 | 0 | |a 1-PHOSPHATIDYLINOSITOL 3-KINASE |
| 690 | 1 | 0 | |a ADAPTOR PROTEINS, SIGNAL TRANSDUCING |
| 690 | 1 | 0 | |a CELL LINE, TUMOR |
| 690 | 1 | 0 | |a EUKARYOTIC INITIATION FACTOR-4E |
| 690 | 1 | 0 | |a FEMALE |
| 690 | 1 | 0 | |a HUMANS |
| 690 | 1 | 0 | |a LEPTIN |
| 690 | 1 | 0 | |a MAP KINASE SIGNALING SYSTEM |
| 690 | 1 | 0 | |a MITOGEN-ACTIVATED PROTEIN KINASE KINASES |
| 690 | 1 | 0 | |a PHOSPHOPROTEINS |
| 690 | 1 | 0 | |a PHOSPHORYLATION |
| 690 | 1 | 0 | |a PREGNANCY |
| 690 | 1 | 0 | |a PROTEIN BIOSYNTHESIS |
| 690 | 1 | 0 | |a PROTO-ONCOGENE PROTEINS C-AKT |
| 690 | 1 | 0 | |a RIBOSOMAL PROTEIN S6 KINASES, 70-KDA |
| 690 | 1 | 0 | |a TROPHOBLASTS |
| 650 | 1 | 7 | |2 spines |a PLACENTA |
| 700 | 1 | |a Gambino, Y. | |
| 700 | 1 | |a Maymó, J. | |
| 700 | 1 | |a Goberna, R. | |
| 700 | 1 | |a Fabiani, F. | |
| 700 | 1 | |a Varone, C. | |
| 700 | 1 | |a Sánchez-Margalet, V. | |
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