Commercial cell wall hydrolytic enzymes for producing pectin-enriched products from butternut (Cucurbita moschata, Duchesne ex Poiret)

A cell wall-enriched butternut (Cucurbita moschata Duch ex Poiret) powder was obtained by drying mesocarp wastes; this powder was used as the substrate for commercial cellulase (A-product) or hemicellulase (B-product) action. Yields were 4.70% and 6.12%, respectively. Both pectin-enriched products w...

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Autor principal: Fissore, E.N
Otros Autores: Ponce, N.M, Wider, E.A, Stortz, C.A, Gerschenson, L.N, Rojas, A.M
Formato: Capítulo de libro
Lenguaje:Inglés
Publicado: 2009
Acceso en línea:Registro en Scopus
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100 1 |a Fissore, E.N. 
245 1 0 |a Commercial cell wall hydrolytic enzymes for producing pectin-enriched products from butternut (Cucurbita moschata, Duchesne ex Poiret) 
260 |c 2009 
270 1 0 |m Gerschenson, L.N.; Industry Department, School of Natural and Exact Sciences (FCEN), Buenos Aires University (UBA)Ciudad Universitaria, Intendente Güiraldes 2620, 1428 Ciudad Autonoma de Buenos Aires, Argentina; email: lia@di.fcen.uba.ar 
506 |2 openaire  |e Política editorial 
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520 3 |a A cell wall-enriched butternut (Cucurbita moschata Duch ex Poiret) powder was obtained by drying mesocarp wastes; this powder was used as the substrate for commercial cellulase (A-product) or hemicellulase (B-product) action. Yields were 4.70% and 6.12%, respectively. Both pectin-enriched products were water soluble fibers and only the latter showed important thickening effect at 20 g dm-3-concentration in water. This product was a highly methylated (methylation degree, DM, 72.6) pectin with 45.2 g/100 g of galacturonic acid and 11 g/100 g of neutral sugars. Cellulase produced intense hydrolysis leading to a less methylated (DM: 54.2) A-fraction. It was observed that not only average molecular weight, but also the molecular structure derived from chemical composition affected flow behavior and viscosity enhancing capacity. As A-fraction was enriched in rhamnogalacturonan I, probably, rhamnose kinks were responsible for the poor thickening effect at 20 g dm-3-concentration in water, in spite of its homogeneous molecular weight profile, which showed only one peak at 793,000. This fraction presented the lowest density of macromolecular interactions in water, behaving as a diluted hydrocolloid solution as confirmed by its fitting to the Cox-Merz rule. Both isolated soluble fractions produced a delay in the glucose dialysis assay. © 2009 Elsevier Ltd. All rights reserved.  |l eng 
536 |a Detalles de la financiación: Universidad de Buenos Aires 
536 |a Detalles de la financiación: Umweltbundesamt 
536 |a Detalles de la financiación: Agencia Nacional de Promoción Científica y Tecnológica 
536 |a Detalles de la financiación: Consejo Nacional de Investigaciones Científicas y Técnicas 
536 |a Detalles de la financiación: The authors acknowledge the financial support from Buenos Aires University (UBA), National Agency of Scientific and Technological Promotion-Argentina (ANPCyT) and National Scientific and Technical Research Council-Argentina (CONICET). DSM Specialties Argentina is also acknowledged for the provision of the enzymes herein used. 
593 |a Industry Department, School of Natural and Exact Sciences (FCEN), Buenos Aires University (UBA)Ciudad Universitaria, Intendente Güiraldes 2620, 1428 Ciudad Autonoma de Buenos Aires, Argentina 
593 |a Organic Chemistry Department-CIHIDECAR, School of Natural and Exact Sciences (FCEN), Buenos Aires University (UBA)Ciudad Universitaria, Intendente Güiraldes 2620, 1428 Ciudad Autonoma de Buenos Aires, Argentina 
593 |a Biological Chemistry Department, School of Natural and Exact Sciences (FCEN), Buenos Aires University (UBA)Ciudad Universitaria, Intendente Güiraldes 2620, 1428 Ciudad Autonoma de Buenos Aires, Argentina 
690 1 0 |a BUTTERNUT 
690 1 0 |a COMPOSITION 
690 1 0 |a ENZYMES 
690 1 0 |a PECTIN 
690 1 0 |a PROPERTIES 
690 1 0 |a CATALYSTS 
690 1 0 |a DIALYSIS 
690 1 0 |a ENZYMES 
690 1 0 |a GLUCOSE 
690 1 0 |a MOLECULAR WEIGHT 
690 1 0 |a POWDERS 
690 1 0 |a SUGAR (SUCROSE) 
690 1 0 |a AVERAGE MOLECULAR WEIGHTS 
690 1 0 |a BUTTERNUT 
690 1 0 |a CELL WALLS 
690 1 0 |a CHEMICAL COMPOSITIONS 
690 1 0 |a COMPOSITION 
690 1 0 |a COX-MERZ RULES 
690 1 0 |a CUCURBITA MOSCHATA 
690 1 0 |a ENHANCING CAPACITIES 
690 1 0 |a FLOW BEHAVIORS 
690 1 0 |a GALACTURONIC ACIDS 
690 1 0 |a HEMICELLULASE 
690 1 0 |a HYDROLYTIC ENZYMES 
690 1 0 |a NEUTRAL SUGARS 
690 1 0 |a PECTIN 
690 1 0 |a PROPERTIES 
690 1 0 |a RHAMNOGALACTURONAN-I 
690 1 0 |a SOLUBLE FRACTIONS 
690 1 0 |a WATER-SOLUBLE FIBERS 
690 1 0 |a POLYSACCHARIDES 
690 1 0 |a CUCURBITA MOSCHATA 
690 1 0 |a JUGLANS CINEREA 
700 1 |a Ponce, N.M. 
700 1 |a Wider, E.A. 
700 1 |a Stortz, C.A. 
700 1 |a Gerschenson, L.N. 
700 1 |a Rojas, A.M. 
773 0 |d 2009  |g v. 93  |h pp. 293-301  |k n. 3  |p J Food Eng  |x 02608774  |w (AR-BaUEN)CENRE-5580  |t Journal of Food Engineering 
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856 4 0 |u https://doi.org/10.1016/j.jfoodeng.2009.01.024  |y DOI 
856 4 0 |u https://hdl.handle.net/20.500.12110/paper_02608774_v93_n3_p293_Fissore  |y Handle 
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