Vero cells persistently infected with tacaribe virus: role of interfering particles in the establishment of the infection

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Eight Vero cell sublines (Vero T) persistently infected with wild type Tacaribe virus replicated in different hosts were established. In order to unravel the mecha- nism involved in the initiation and maintenance of persistence, the properties of virus shed by the sublines and the presence of interf...

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Autor principal: D'Aiutolo, A.C
Otros Autores: Coto, C.E
Formato: Capítulo de libro
Lenguaje:Inglés
Publicado: 1986
Acceso en línea:Registro en Scopus
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Aporte de:Registro referencial: Solicitar el recurso aquí
Biblioteca Central Dr. Luis F. Leloir (FCEN) de Universidad de Buenos Aires
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100 1 |a D'Aiutolo, A.C. 
245 1 0 |a Vero cells persistently infected with tacaribe virus: role of interfering particles in the establishment of the infection 
260 |c 1986 
270 1 0 |m D'Aiutolo, A.C.; Laboratorio de Virología, Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Ciudad Universitaria, Pabellon II, Piso 4, 1428 Buenos Aires, Argentina 
506 |2 openaire  |e Política editorial 
504 |a Candurra, Damonte, Influence of cellular functions on the evolution of persistent infections with Junin virus (1985) Arch. Virol., 86, pp. 275-282 
504 |a Coto, León, Peralta, Help, Laguens, Induction of infectious virus and viral surface antigens in Vero cells persistently infected with Junin virus (1979) Humoral Immunity and Neurological Diseases, pp. 405-415. , D. Karcher, A. Lowenthal, A.D. Strosberg, Plenum Press, New York 
504 |a Damonte, Coto, Análisis de los factores que condicionan la formación de placas en células Vero infectadas con virus Junín y Tacaribe (1979) Revta. Asoc. Arg. Microbiol., 6, pp. 15-22 
504 |a Damonte, Coto, Temperature sensitivity of the arenavirus Junin isolated from persistently infected Vero cells (1979) Intervirology, 11, pp. 282-287 
504 |a Damonte, D'Aiutolo, Coto, Persistent infection of Tacaribe virus in Vero cells (1981) J. Gen. Virol., 65, pp. 41-48 
504 |a Damonte, Mersich, Coto, Response of cells persistently infected with Arenaviruses to superinfection with homotypic and heterotypic viruses (1983) Virology, 129, pp. 474-478 
504 |a Friedman, Ramseur, Mechanisms of persistent infections by cytopathic viruses in tissue culture (1979) Arch. Virol., 60, pp. 83-103 
504 |a Gimenez, Compans, Defective interfering Tacaribe virus and persistently infected cells (1980) Virology, 107, pp. 229-239 
504 |a Help, Coto, Propiedades de las partículas interferentes generadas por el virus Junín en células Vero (1981) Medicina (Bs. As.), 41, pp. 19-24 
504 |a Holland, Levine, Mechanisms of virus persistence (1979) Cell, 14, pp. 447-452 
504 |a Holland, Spindler, Horodyski, Graham, Nochol, Vandepol, Rapid evolution of RNA genomes (1982) Science, 215, pp. 1577-1585 
504 |a Jacobson, Dutko, Pfau, Determinants of spontaneous recovery and persistence in MDCK cells infected with LCM virus (1979) J. Gen. Virol., 44, pp. 113-121 
504 |a Oldstone, Holmstoen, Welsh, Jr., Alterations in acetylcholine enzyme in neuroblastoma cells persistently infected with LCM virus (1977) J. Cell. Physiol., 91, pp. 459-472 
504 |a Preble, Youngner, ts viruses and etiology of chronic and inapparent infections (1975) J. Infect. Dis., 131, pp. 467-473 
504 |a Rawls, Chan, Gee, Mechanisms of persistence in arenavirus infection: A brief report (1981) Can. J. Microbiol., 27, pp. 568-574 
504 |a Ron, Tal, Coevolution of cells and virus as a mechanism of the persistence of Lymphotropic Minute virus of mice in L-cells (1985) J. Virol., 55, pp. 424-430 
504 |a Sekellick, Marcus, Persistent infection. II. Interferon inducing temperature sensitive mutants as mediators of cells sparing: Possible role in persistent infection by vesicular stomatitis virus (1979) Virology, 95, pp. 36-47 
504 |a Youngner, Preble, Evolution of viral populations (1980) Comprehensive Virology, 16, pp. 73-135. , H. Fraenkel-Conrat, R.R. Wagner, Plenum Press, New York 
520 3 |a Eight Vero cell sublines (Vero T) persistently infected with wild type Tacaribe virus replicated in different hosts were established. In order to unravel the mecha- nism involved in the initiation and maintenance of persistence, the properties of virus shed by the sublines and the presence of interfering particles (IP) were analyzed. During the course of infection, persistent virus (Tac-pi) underwent muta- tions although no consistent pattern of virus evolution was observed, ts mutants were isolated from two Vero T sublines, whereas a slow growth variant was shed by another. The remaining sublines released virus resembling wt parental virus. Except for Vero T1 sublines, Vero T cultures shed no detectable IP. These results emphasize the point that neither the emergence of virus mutants nor the synthesis of IP is essential for the maintenance of the persistent state. To define the role of IP in the initiation of persistence, coinfection experiments with a characterized inoculum were performed. For that purpose, attempts were made to obtain IP stocks free from pfu by serial transfers of undiluted virus. Neither enrichment nor amplification of IP occurred, and virus stocks were freed of infectious virus by UV irradiation. If normal Vero cells were infected with Tac-pi virus released by Vero T2, Vero T3, Vero T4, Vero T5, Vero T6, Vero T7 and Vero T10 sublines, a complete destruction of the monolayer without cell recovery was ob- served. In contrast, parental and Vero T1 viruses always originated persistently infected sublines. Similarly, the addition of IP to virus inocula constituted by Tac-pi viruses released by Vero T2, Vero T3, Vero T4, Vero T5, Vero T6, Vero T7 and Vero T10 sublines gave rise to persistently infected cultures. These results suggest that although IP are not important by themselves in the maintenance of persistence, they play a major role in initiation. © 1986.  |l eng 
593 |a Laboratorio de Virología, Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Ciudad Universitaria, Pabellon II, Piso 4, 1428 Buenos Aires, Argentina 
690 1 0 |a INTERFERING PARTICLES 
690 1 0 |a PERSISTENT INFECTION 
690 1 0 |a TACARIBE VIRUS 
690 1 0 |a VERO CELLS 
690 1 0 |a CELL CULTURE 
690 1 0 |a HEREDITY 
690 1 0 |a IN VITRO STUDY 
690 1 0 |a MONKEY 
690 1 0 |a NONHUMAN 
690 1 0 |a PERSISTENT INFECTION 
690 1 0 |a PRIORITY JOURNAL 
690 1 0 |a TACARIBE ARENAVIRUS 
690 1 0 |a TEMPERATURE SENSITIVE MUTANT 
690 1 0 |a VIRUS INTERFERENCE 
690 1 0 |a VIRUS ISOLATION 
690 1 0 |a ANIMAL 
690 1 0 |a ANIMALS, SUCKLING 
690 1 0 |a ARENAVIRIDAE 
690 1 0 |a ARENAVIRUSES, NEW WORLD 
690 1 0 |a MICE 
690 1 0 |a SUPPORT, NON-U.S. GOV'T 
690 1 0 |a VERO CELLS 
690 1 0 |a VIRAL INTERFERENCE 
690 1 0 |a VIRUS REPLICATION 
700 1 |a Coto, C.E. 
773 0 |d 1986  |g v. 6  |h pp. 235-244  |k n. 3  |p Virus Res.  |x 01681702  |w (AR-BaUEN)CENRE-7132  |t Virus Research 
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856 4 0 |u https://hdl.handle.net/20.500.12110/paper_01681702_v6_n3_p235_DAiutolo  |y Handle 
856 4 0 |u https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01681702_v6_n3_p235_DAiutolo  |y Registro en la Biblioteca Digital 
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