Testing two types of molecular methods for the detection of Candidatus Liberibacter, the causative agent of huanglongbing (HLB) in the greening of citrus fruits in Ecuador.
Citrus greening disease, caused by the pathogenic bacterium Candidatus Liberibacter, is a looming problem in Ecuador, as it is present in neighbouring countries. The damage caused by this disease disrupts phloem function and severely impairs the translocation of assimilates in the host plant, often...
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| Formato: | Artículo revista |
| Lenguaje: | Español |
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Faculta de Ciencias Agropecuarias. Secretaría de Ciencia y Tecnología.
2024
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| Acceso en línea: | https://revistas.unc.edu.ar/index.php/nexoagro/article/view/44750 |
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I10-R308-article-44750 |
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I10-R308-article-447502024-12-23T13:32:00Z Testing two types of molecular methods for the detection of Candidatus Liberibacter, the causative agent of huanglongbing (HLB) in the greening of citrus fruits in Ecuador. Testing two types of molecular methods for the detection of Candidatus liberibacter, the causative agent of Huanglongbing (HLB) in the greening of citrus fruits in Ecuador Chevez Vera, Héctor David Pinargote-Mendoza, Edgar Rodolfo Herrera-Jácome, Darío Fernando Mehdi Jazayeri, Seyed Villamar Torres, Ronald Oswaldo Villamar Torres, Ronald Oswaldo Candidatus Liberibacter, HLB, DNA, Electrophoresis, Citrus Candidatus Liberibacter HLB DNA Electrophoresis citrus Citrus greening disease, caused by the pathogenic bacterium Candidatus Liberibacter, is a looming problem in Ecuador, as it is present in neighbouring countries. The damage caused by this disease disrupts phloem function and severely impairs the translocation of assimilates in the host plant, often leading to death. To accurately identify the causative bacterium of citrus greening using molecular techniques and a rapid-action protocol, clustered sampling was conducted on symptomatic plants in four provinces of Ecuador, where the vector is already present and citrus-producing areas exist. Two methodologies, CTAB DNA extraction and the DNA-easy plant mini kit, were employed to extract DNA for subsequent verification of the presence/absence of this microorganism through PCR, followed by electrophoresis and cost analysis. Results showed similar DNA extractions using both methods, with quantification through electrophoresis and spectrophotometry indicating a positive correlation of r2=0.9402. PCR reaction with the 16S rRNA molecular markers of the 22 samples did not detect the presence of the bacterium in any of the areas. The total diagnostic cost for PCR of 18 samples was determined to be $84.93, with a unit value of $4.71. Using the Qiagen® DNA-easy plant mini method, the unit value per sample was $3.36, resulting in a total cost of $60.65 for the CTAB method. Citrus greening disease, caused by the pathogenic bacterium Candidatus Liberibacter, is a looming problem in Ecuador, as it is present in neighbouring countries. The damage caused by this disease disrupts phloem function and severely impairs the translocation of assimilates in the host plant, often leading to death. To accurately identify the causative bacterium of citrus greening using molecular techniques and a rapid-action protocol, clustered sampling was conducted on symptomatic plants in four provinces of Ecuador, where the vector is already present and citrus-producing areas exist. Two methodologies, CTAB DNA extraction and the DNA-easy plant mini kit, were employed to extract DNA for subsequent verification of the presence/absence of this microorganism through PCR, followed by electrophoresis and cost analysis. Results showed similar DNA extractions using both methods, with quantification through electrophoresis and spectrophotometry indicating a positive correlation of r2=0.9402. PCR reaction with the 16S rRNA molecular markers of the 22 samples did not detect the presence of the bacterium in any of the areas. The total diagnostic cost for PCR of 18 samples was determined to be $84.93, with a unit value of $4.71. Using the Qiagen® DNA-easy plant mini method, the unit value per sample was $3.36, resulting in a total cost of $60.65 for the CTAB method. Faculta de Ciencias Agropecuarias. Secretaría de Ciencia y Tecnología. 2024-07-25 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://revistas.unc.edu.ar/index.php/nexoagro/article/view/44750 Nexo agropecuario; Vol. 12 Núm. 1 (2024); 36-45 2346-917X 2346-9110 spa https://revistas.unc.edu.ar/index.php/nexoagro/article/view/44750/45872 Derechos de autor 2024 Héctor David Chevez Vera; Edgar Rodolfo Pinargote-Mendoza; Darío Fernando Herrera-Jácome, Seyed Mehdi Jazayeri, Ronald Oswaldo Villamar Torres https://creativecommons.org/licenses/by-nc-sa/4.0 |
| institution |
Universidad Nacional de Córdoba |
| institution_str |
I-10 |
| repository_str |
R-308 |
| container_title_str |
Nexo agropecuario |
| language |
Español |
| format |
Artículo revista |
| topic |
Candidatus Liberibacter, HLB, DNA, Electrophoresis, Citrus Candidatus Liberibacter HLB DNA Electrophoresis citrus |
| spellingShingle |
Candidatus Liberibacter, HLB, DNA, Electrophoresis, Citrus Candidatus Liberibacter HLB DNA Electrophoresis citrus Chevez Vera, Héctor David Pinargote-Mendoza, Edgar Rodolfo Herrera-Jácome, Darío Fernando Mehdi Jazayeri, Seyed Villamar Torres, Ronald Oswaldo Villamar Torres, Ronald Oswaldo Testing two types of molecular methods for the detection of Candidatus Liberibacter, the causative agent of huanglongbing (HLB) in the greening of citrus fruits in Ecuador. |
| topic_facet |
Candidatus Liberibacter, HLB, DNA, Electrophoresis, Citrus Candidatus Liberibacter HLB DNA Electrophoresis citrus |
| author |
Chevez Vera, Héctor David Pinargote-Mendoza, Edgar Rodolfo Herrera-Jácome, Darío Fernando Mehdi Jazayeri, Seyed Villamar Torres, Ronald Oswaldo Villamar Torres, Ronald Oswaldo |
| author_facet |
Chevez Vera, Héctor David Pinargote-Mendoza, Edgar Rodolfo Herrera-Jácome, Darío Fernando Mehdi Jazayeri, Seyed Villamar Torres, Ronald Oswaldo Villamar Torres, Ronald Oswaldo |
| author_sort |
Chevez Vera, Héctor David |
| title |
Testing two types of molecular methods for the detection of Candidatus Liberibacter, the causative agent of huanglongbing (HLB) in the greening of citrus fruits in Ecuador. |
| title_short |
Testing two types of molecular methods for the detection of Candidatus Liberibacter, the causative agent of huanglongbing (HLB) in the greening of citrus fruits in Ecuador. |
| title_full |
Testing two types of molecular methods for the detection of Candidatus Liberibacter, the causative agent of huanglongbing (HLB) in the greening of citrus fruits in Ecuador. |
| title_fullStr |
Testing two types of molecular methods for the detection of Candidatus Liberibacter, the causative agent of huanglongbing (HLB) in the greening of citrus fruits in Ecuador. |
| title_full_unstemmed |
Testing two types of molecular methods for the detection of Candidatus Liberibacter, the causative agent of huanglongbing (HLB) in the greening of citrus fruits in Ecuador. |
| title_sort |
testing two types of molecular methods for the detection of candidatus liberibacter, the causative agent of huanglongbing (hlb) in the greening of citrus fruits in ecuador. |
| description |
Citrus greening disease, caused by the pathogenic bacterium Candidatus Liberibacter, is a looming problem in Ecuador, as it is present in neighbouring countries. The damage caused by this disease disrupts phloem function and severely impairs the translocation of assimilates in the host plant, often leading to death. To accurately identify the causative bacterium of citrus greening using molecular techniques and a rapid-action protocol, clustered sampling was conducted on symptomatic plants in four provinces of Ecuador, where the vector is already present and citrus-producing areas exist. Two methodologies, CTAB DNA extraction and the DNA-easy plant mini kit, were employed to extract DNA for subsequent verification of the presence/absence of this microorganism through PCR, followed by electrophoresis and cost analysis. Results showed similar DNA extractions using both methods, with quantification through electrophoresis and spectrophotometry indicating a positive correlation of r2=0.9402. PCR reaction with the 16S rRNA molecular markers of the 22 samples did not detect the presence of the bacterium in any of the areas. The total diagnostic cost for PCR of 18 samples was determined to be $84.93, with a unit value of $4.71. Using the Qiagen® DNA-easy plant mini method, the unit value per sample was $3.36, resulting in a total cost of $60.65 for the CTAB method. |
| publisher |
Faculta de Ciencias Agropecuarias. Secretaría de Ciencia y Tecnología. |
| publishDate |
2024 |
| url |
https://revistas.unc.edu.ar/index.php/nexoagro/article/view/44750 |
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