Production and characterization of amphidiploids from hybrids between Arachis ipaënsis and A. duranensis (Fabaceae): implications for peanut improvement

Background and aims: Arachis hypogaea is an allotetraploid species of global economic importance; however, its narrow genetic base renders it vulnerable to pests and diseases. In contrast, wild diploid Arachis species possess valuable resistance alleles, but ploidy differences limit their direct use...

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Autores principales: García, Alejandra V., Ortiz, Alejandra Marcela, Arcangeli , Julieta, Lavia, Graciela I., Ortiz, Alejandra M.
Formato: Artículo revista
Lenguaje:Inglés
Español
Publicado: Sociedad Argentina de Botánica 2026
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Acceso en línea:https://revistas.unc.edu.ar/index.php/BSAB/article/view/50235
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institution Universidad Nacional de Córdoba
institution_str I-10
repository_str R-325
container_title_str Boletín de la Sociedad Argentina de Botánica
language Inglés
Español
format Artículo revista
topic Alotetraploides sintéticos
Arachis
citogenética
duplicación cromosómica
pre-mejoramiento
Arachis
chromosome doubling
cytogenetics
pre-breeding
synthetic allotetraploids
spellingShingle Alotetraploides sintéticos
Arachis
citogenética
duplicación cromosómica
pre-mejoramiento
Arachis
chromosome doubling
cytogenetics
pre-breeding
synthetic allotetraploids
García, Alejandra V.
Ortiz, Alejandra Marcela
Arcangeli , Julieta
Lavia, Graciela I.
Ortiz, Alejandra M.
Production and characterization of amphidiploids from hybrids between Arachis ipaënsis and A. duranensis (Fabaceae): implications for peanut improvement
topic_facet Alotetraploides sintéticos
Arachis
citogenética
duplicación cromosómica
pre-mejoramiento
Arachis
chromosome doubling
cytogenetics
pre-breeding
synthetic allotetraploids
author García, Alejandra V.
Ortiz, Alejandra Marcela
Arcangeli , Julieta
Lavia, Graciela I.
Ortiz, Alejandra M.
author_facet García, Alejandra V.
Ortiz, Alejandra Marcela
Arcangeli , Julieta
Lavia, Graciela I.
Ortiz, Alejandra M.
author_sort García, Alejandra V.
title Production and characterization of amphidiploids from hybrids between Arachis ipaënsis and A. duranensis (Fabaceae): implications for peanut improvement
title_short Production and characterization of amphidiploids from hybrids between Arachis ipaënsis and A. duranensis (Fabaceae): implications for peanut improvement
title_full Production and characterization of amphidiploids from hybrids between Arachis ipaënsis and A. duranensis (Fabaceae): implications for peanut improvement
title_fullStr Production and characterization of amphidiploids from hybrids between Arachis ipaënsis and A. duranensis (Fabaceae): implications for peanut improvement
title_full_unstemmed Production and characterization of amphidiploids from hybrids between Arachis ipaënsis and A. duranensis (Fabaceae): implications for peanut improvement
title_sort production and characterization of amphidiploids from hybrids between arachis ipaënsis and a. duranensis (fabaceae): implications for peanut improvement
description Background and aims: Arachis hypogaea is an allotetraploid species of global economic importance; however, its narrow genetic base renders it vulnerable to pests and diseases. In contrast, wild diploid Arachis species possess valuable resistance alleles, but ploidy differences limit their direct use in breeding. Syntheticamphidiploids overcome this barrier, however, BBAA amphidiploids compatible with peanut are not available in Argentina. This study aimed to develop amphidiploidindividuals suitable for national pre-breeding efforts.  M&M: Two chromosome duplication protocols were evaluated in hybrids [A. ipaënsis × A. duranensis]2x based on partial or complete immersion of cuttings in a 0.2 % colchicine solution. Ploidy level was verified by flow cytometry and genome composition was confirmed by DAPI banding. Pollen viability was estimated via acetocarmine staining. Fruit characterization was performed using Fiji software.  Results: Flow cytometry confirmed that only protocol II yielded tetraploid individuals. DAPI banding revealed the expected BBAA genome configuration, and restoredfertility was demonstrated by high pollen viability and fruit production. Morphologically,  amphidiploids showed vigorous growth and increased fruit size. Conclusions: Fertile BBAA amphidiploids were developed as a strategic resource for peanut pre- reeding in Argentina. Additionally, it will support studies on polyploid genome stabilization, and trait expression following hybridization and chromosome duplication in Arachis.
publisher Sociedad Argentina de Botánica
publishDate 2026
url https://revistas.unc.edu.ar/index.php/BSAB/article/view/50235
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first_indexed 2026-04-27T05:46:04Z
last_indexed 2026-04-27T05:46:04Z
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spelling I10-R325-article-502352026-04-06T14:39:37Z Production and characterization of amphidiploids from hybrids between Arachis ipaënsis and A. duranensis (Fabaceae): implications for peanut improvement Producción y caracterización de anfidiploides a partir de híbridos entre Arachis ipaënsis y A. duranensis (Fabaceae): implicaciones para el mejoramiento del maní García, Alejandra V. Ortiz, Alejandra Marcela Arcangeli , Julieta Lavia, Graciela I. Ortiz, Alejandra M. Alotetraploides sintéticos Arachis citogenética duplicación cromosómica pre-mejoramiento Arachis chromosome doubling cytogenetics pre-breeding synthetic allotetraploids Background and aims: Arachis hypogaea is an allotetraploid species of global economic importance; however, its narrow genetic base renders it vulnerable to pests and diseases. In contrast, wild diploid Arachis species possess valuable resistance alleles, but ploidy differences limit their direct use in breeding. Syntheticamphidiploids overcome this barrier, however, BBAA amphidiploids compatible with peanut are not available in Argentina. This study aimed to develop amphidiploidindividuals suitable for national pre-breeding efforts.  M&M: Two chromosome duplication protocols were evaluated in hybrids [A. ipaënsis × A. duranensis]2x based on partial or complete immersion of cuttings in a 0.2 % colchicine solution. Ploidy level was verified by flow cytometry and genome composition was confirmed by DAPI banding. Pollen viability was estimated via acetocarmine staining. Fruit characterization was performed using Fiji software.  Results: Flow cytometry confirmed that only protocol II yielded tetraploid individuals. DAPI banding revealed the expected BBAA genome configuration, and restoredfertility was demonstrated by high pollen viability and fruit production. Morphologically,  amphidiploids showed vigorous growth and increased fruit size. Conclusions: Fertile BBAA amphidiploids were developed as a strategic resource for peanut pre- reeding in Argentina. Additionally, it will support studies on polyploid genome stabilization, and trait expression following hybridization and chromosome duplication in Arachis. Introducción y objetivos: Arachis hypogaea es un alotetraploide de importancia económica mundial, cuya base genética estrecha lo hace vulnerable a plagas y enfermedades. Las especies silvestres diploides de Arachis poseen alelos de resistencia, pero las diferencias de ploidía limitan su uso directo en el mejoramiento. Los anfidiploides sintéticos superan esta barrera, sin embargo, anfidiploides BBAA compatibles con el maní no están disponibles en Argentina. El objetivo de trabajo  fue desarrollar anfidiploides para los programas nacionales de pre-mejoramiento. M&M: Se evaluaron dos protocolos de duplicación cromosómica en híbridos [A. ipaënsis × A. duranensis] 2x basados en inmersión parcial o completa de estacas en solución de colchicina al 0,2 %. El nivel de ploidía se determinó mediante citometría de flujo y la composición genómica por bandeo DAPI. La viabilidad se estimó mediante tinción con aceto-carmín. La caracterización de frutos se realizó con el software Fiji. Resultados: La citometría de flujo confirmó que solo el protocolo II produjo individuos tetraploides. El bandeo DAPI reveló la configuración genómica BBAA, y la fertilidad se evidenció por la alta viabilidad polínica y producción de frutos.  Morfológicamente, los anfidiploides mostraron crecimiento vigoroso y aumento del tamaño de los frutos. Conclusiones: Se desarrollaron anfidiploides BBAA fértiles como recurso estratégico para el pre-mejoramiento del maní en Argentina. Además, servirá de apoyo a estudios sobre estabilización de genomas poliploides, y expresión de rasgos post hibridación y duplicación cromosómica en Arachis. Sociedad Argentina de Botánica 2026-03-31 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf text/html https://revistas.unc.edu.ar/index.php/BSAB/article/view/50235 10.31055/1851.2372.v61.n1.50235 Boletín de la Sociedad Argentina de Botánica (Journal of the Argentine Botanical Society); Vol. 61 No. 1 (2026): March Boletín de la Sociedad Argentina de Botánica; Vol. 61 Núm. 1 (2026): Marzo Boletín de la Sociedad Argentina de Botánica; v. 61 n. 1 (2026): Marzo 1851-2372 0373-580X 10.31055/1851.2372.v61.n1 eng spa https://revistas.unc.edu.ar/index.php/BSAB/article/view/50235/52787 https://revistas.unc.edu.ar/index.php/BSAB/article/view/50235/52788 Derechos de autor 2026 Alejandra V. García, Alejandra M. Ortiz, Julieta Arcangeli , Graciela I. Lavia https://creativecommons.org/licenses/by-nc-sa/4.0