Use of the fluorescent in situ hybridization technique for the rapid identification of staphylococcus aureus in blood cultures

Background: The technique of reference for the detection of bacteriemia is the blood culture. One of the most frequent bacteria responsible of bacteriemia is Staphylococcus aureus (SAU). The presence of Gram positive cocci (CGPR), in blood culture give the suspicious of bacteriemia, although the rel...

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Autores principales: Cómito, Maria L., Vilaró, Mario, Cuestas, Eduardo, Moscone, Eduardo
Formato: Artículo revista
Lenguaje:Español
Publicado: Universidad Nacional Córdoba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología 2009
Materias:
blood cultures
bacteriemia
Staphylococcus
rapid identification
FISH
hemocultivos
identificación rápida
Acceso en línea:https://revistas.unc.edu.ar/index.php/med/article/view/23457
Aporte de:
Revista de la Facultad de Ciencias Médicas de Córdoba de Universidad Nacional de Córdoba
id I10-R327-article-23457
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institution Universidad Nacional de Córdoba
institution_str I-10
repository_str R-327
container_title_str Revista de la Facultad de Ciencias Médicas de Córdoba
language Español
format Artículo revista
topic blood cultures
bacteriemia
Staphylococcus
rapid identification
FISH
hemocultivos
bacteriemia
Staphylococcus
identificación rápida
FISH
spellingShingle blood cultures
bacteriemia
Staphylococcus
rapid identification
FISH
hemocultivos
bacteriemia
Staphylococcus
identificación rápida
FISH
Cómito, Maria L.
Vilaró, Mario
Cuestas, Eduardo
Moscone, Eduardo
Use of the fluorescent in situ hybridization technique for the rapid identification of staphylococcus aureus in blood cultures
topic_facet blood cultures
bacteriemia
Staphylococcus
rapid identification
FISH
hemocultivos
bacteriemia
Staphylococcus
identificación rápida
FISH
author Cómito, Maria L.
Vilaró, Mario
Cuestas, Eduardo
Moscone, Eduardo
author_facet Cómito, Maria L.
Vilaró, Mario
Cuestas, Eduardo
Moscone, Eduardo
author_sort Cómito, Maria L.
title Use of the fluorescent in situ hybridization technique for the rapid identification of staphylococcus aureus in blood cultures
title_short Use of the fluorescent in situ hybridization technique for the rapid identification of staphylococcus aureus in blood cultures
title_full Use of the fluorescent in situ hybridization technique for the rapid identification of staphylococcus aureus in blood cultures
title_fullStr Use of the fluorescent in situ hybridization technique for the rapid identification of staphylococcus aureus in blood cultures
title_full_unstemmed Use of the fluorescent in situ hybridization technique for the rapid identification of staphylococcus aureus in blood cultures
title_sort use of the fluorescent in situ hybridization technique for the rapid identification of staphylococcus aureus in blood cultures
description Background: The technique of reference for the detection of bacteriemia is the blood culture. One of the most frequent bacteria responsible of bacteriemia is Staphylococcus aureus (SAU). The presence of Gram positive cocci (CGPR), in blood culture give the suspicious of bacteriemia, although the relevance of the finding depends on the correct identification of the microorganism. The definitive typification it demands from 24 to 48 hours. The technique of fluorescent in situ hybridization (FISH), is a rapid method for the identification of bacteria in blood culture. Aims: The aims of this work was to establish the FISH technique directed to the 16S RNA of SAU in samples of blood cultures with CGPR to the Gram staining, to determine the concordance with the method of reference, and to evaluate the possibility to use as standard method in the laboratory of microbiology. Material and Methods: The blood cultures analyzed in the Laboratory of Microbiology of the Hospital Privado Cordoba from 01.01.2009 to 31.12.09 were included in this study. To each positive blood culture which showed CGPR was applied simultaneously biochemical identification and FISH. Results: From 496 positive samples, 32 showed CGPR, 24 of them were identified as SAU. A concordance of 100% (IC95% 99 to 100) between both methods of diagnosis, with a value of k<0.01 was observed. Discussion: The excellent concordance opens the prospect for a study in order to determine the predictive value of FISH, its sensibility and specificity. Furthermore, it will be possible to evaluate its clinical impact being incorporated in a laboratory of clinical bacteriology.
publisher Universidad Nacional Córdoba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología
publishDate 2009
url https://revistas.unc.edu.ar/index.php/med/article/view/23457
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spelling I10-R327-article-234572024-08-27T18:24:26Z Use of the fluorescent in situ hybridization technique for the rapid identification of staphylococcus aureus in blood cultures Uso de la técnica de hibridación fluorescente in situ para la identificación rápida de staphylococcus aureus en hemocultivos Cómito, Maria L. Vilaró, Mario Cuestas, Eduardo Moscone, Eduardo blood cultures bacteriemia Staphylococcus rapid identification FISH hemocultivos bacteriemia Staphylococcus identificación rápida FISH Background: The technique of reference for the detection of bacteriemia is the blood culture. One of the most frequent bacteria responsible of bacteriemia is Staphylococcus aureus (SAU). The presence of Gram positive cocci (CGPR), in blood culture give the suspicious of bacteriemia, although the relevance of the finding depends on the correct identification of the microorganism. The definitive typification it demands from 24 to 48 hours. The technique of fluorescent in situ hybridization (FISH), is a rapid method for the identification of bacteria in blood culture. Aims: The aims of this work was to establish the FISH technique directed to the 16S RNA of SAU in samples of blood cultures with CGPR to the Gram staining, to determine the concordance with the method of reference, and to evaluate the possibility to use as standard method in the laboratory of microbiology. Material and Methods: The blood cultures analyzed in the Laboratory of Microbiology of the Hospital Privado Cordoba from 01.01.2009 to 31.12.09 were included in this study. To each positive blood culture which showed CGPR was applied simultaneously biochemical identification and FISH. Results: From 496 positive samples, 32 showed CGPR, 24 of them were identified as SAU. A concordance of 100% (IC95% 99 to 100) between both methods of diagnosis, with a value of k<0.01 was observed. Discussion: The excellent concordance opens the prospect for a study in order to determine the predictive value of FISH, its sensibility and specificity. Furthermore, it will be possible to evaluate its clinical impact being incorporated in a laboratory of clinical bacteriology. Antecedentes: La técnica de referencia para la detección de bacteriemias es el hemocultivo. Uno de los gérmenes que causa bacteriemias con mayor frecuencia es Staphylococcus aureus (SAU). La presencia de cocos Gram positivos en racimos (CGPR) en un hemocultivo es sugestiva de bacteriemia, aunque la relevancia del hallazgo depende de la identificación correcta del microorganismo. La tipificación definitiva demanda entre 24 y 48 hs. La técnica de hibridación fluorescente in situ (FISH), es un método rápido para la identificación de bacterias en hemocultivos. Objetivos: El objetivo de este trabajo fue poner a punto la técnica de FISH dirigido al ARN 16s de SAU, en muestras de hemocultivos con CGPR a la coloración de Gram, determinar la concordancia con el método de referencia y la factibilidad de implementarlo en la rutina del laboratorio de Microbiología. Material y métodos: Se incluyeron los hemocultivos estudiados en el Laboratorio de Microbiología del Hospital Privado de Córdoba, entre el 1/01/09 y 31/12/09. A cada hemocultivo positivo que mostró CGPR se les realizó simultáneamente identificación microbiológica bioquímica y FISH. Resultados: Sobre 496 positivas 32 mostraron CGPR y 24 se identificaron como SAU. Se observó una concordancia de 100% (IC95% 99 a 100) entre ambos métodos diagnósticos, con un valor de ? <0,01. Discusión: Las excelente concordancia obtenida abre la puerta a un estudio para determinar el valor predictivo de FISH, su sensibilidad y especificidad; y evaluar el impacto clínico de su incorporación a un laboratorio de bacteriología clínica. Universidad Nacional Córdoba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología 2009-12-01 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://revistas.unc.edu.ar/index.php/med/article/view/23457 10.31053/1853.0605.v66.n4.23457 Revista de la Facultad de Ciencias Médicas de Córdoba.; Vol. 66 No. 4 (2009); 140-145 Revista de la Facultad de Ciencias Médicas de Córdoba; Vol. 66 Núm. 4 (2009); 140-145 Revista da Faculdade de Ciências Médicas de Córdoba; v. 66 n. 4 (2009); 140-145 1853-0605 0014-6722 10.31053/1853.0605.v66.n4 spa https://revistas.unc.edu.ar/index.php/med/article/view/23457/23203 Derechos de autor 2009 Universidad Nacional de Córdoba https://creativecommons.org/licenses/by-nc/4.0

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