Elicobacter pylori detected in atheroma plaque
Introduction: In general, infection and chronic inflamination have been implied as etiologie agenls for atheroesclerosis and in particular eoronary illness (CI). Several studies have correlated the infeetion of Helicobacter pylori with CI, especially with virulent strains (lineag...
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| Formato: | Artículo revista |
| Lenguaje: | Español |
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Universidad Nacional Córdoba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología
2019
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| Acceso en línea: | https://revistas.unc.edu.ar/index.php/med/article/view/26423 |
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I10-R327-article-26423 |
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ojs |
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Universidad Nacional de Córdoba |
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I-10 |
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R-327 |
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Revista de la Facultad de Ciencias Médicas de Córdoba |
| language |
Español |
| format |
Artículo revista |
| topic |
Helicobacter pylori atherosclerosis atherorna plaque autoimmunity Helicobacter pylori ateroesclerosis placa de ateroma autoinmunidad |
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Helicobacter pylori atherosclerosis atherorna plaque autoimmunity Helicobacter pylori ateroesclerosis placa de ateroma autoinmunidad Guirardi, G Maldonado, F Guzmán, L Juaneda, J Zúñiga, ME Elicobacter pylori detected in atheroma plaque |
| topic_facet |
Helicobacter pylori atherosclerosis atherorna plaque autoimmunity Helicobacter pylori ateroesclerosis placa de ateroma autoinmunidad |
| author |
Guirardi, G Maldonado, F Guzmán, L Juaneda, J Zúñiga, ME |
| author_facet |
Guirardi, G Maldonado, F Guzmán, L Juaneda, J Zúñiga, ME |
| author_sort |
Guirardi, G |
| title |
Elicobacter pylori detected in atheroma plaque |
| title_short |
Elicobacter pylori detected in atheroma plaque |
| title_full |
Elicobacter pylori detected in atheroma plaque |
| title_fullStr |
Elicobacter pylori detected in atheroma plaque |
| title_full_unstemmed |
Elicobacter pylori detected in atheroma plaque |
| title_sort |
elicobacter pylori detected in atheroma plaque |
| description |
Introduction: In general, infection and chronic inflamination have been implied as etiologie agenls for atheroesclerosis and in particular eoronary illness (CI). Several studies have correlated the infeetion of Helicobacter pylori with CI, especially with virulent strains (lineage Cag A). Objective: Demonstrate the immunohistochemical presence of H. Pylori in atheroseletotic plaques obtained from endarterectomy of different vascular regions. Material and rnethods: 34 atherosclerotic plaques of different vascular arcas were studied, (25 men and 9 women). The tissues were fixed with 10% neutral buffered-formalin and decalcifying in formlc acid 5% was used when necessary. The tissue sections were included in paraffin, cut and colored with H&E and subjected to Immunohistochemistry (IHC) of H.Pylori. Briefly, tissues were deparaffinized and thermally treated with a citrate-based solution of antigenic retrieval (ImmunoDNA Retriever with Citrate, BIO SB, Santa Barbara, CA) using a water bath at 95°C for 1 hour. The IHC was conducted using a high sensiUvity Biotin- Streptavidin-HRP-DAB IHC system (ImmunoDetector HRP/DAB, BIO SB). The rnicroscopic observaUon evaluated the presence of mononuclear inflamrnatory celis and the identification of the bacteria in the wall or the vascular lumen. Results: Of the 34 cases studied 14 were positive, where one could identify the bacillus tu their different forms (41,17%) associated with chronic inflammation. |
| publisher |
Universidad Nacional Córdoba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología |
| publishDate |
2019 |
| url |
https://revistas.unc.edu.ar/index.php/med/article/view/26423 |
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2024-09-03T21:01:30Z |
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I10-R327-article-264232024-08-27T18:26:59Z Elicobacter pylori detected in atheroma plaque Detección de helicobacter pylory en placas de ateroma Guirardi, G Maldonado, F Guzmán, L Juaneda, J Zúñiga, ME Helicobacter pylori atherosclerosis atherorna plaque autoimmunity Helicobacter pylori ateroesclerosis placa de ateroma autoinmunidad Introduction: In general, infection and chronic inflamination have been implied as etiologie agenls for atheroesclerosis and in particular eoronary illness (CI). Several studies have correlated the infeetion of Helicobacter pylori with CI, especially with virulent strains (lineage Cag A). Objective: Demonstrate the immunohistochemical presence of H. Pylori in atheroseletotic plaques obtained from endarterectomy of different vascular regions. Material and rnethods: 34 atherosclerotic plaques of different vascular arcas were studied, (25 men and 9 women). The tissues were fixed with 10% neutral buffered-formalin and decalcifying in formlc acid 5% was used when necessary. The tissue sections were included in paraffin, cut and colored with H&E and subjected to Immunohistochemistry (IHC) of H.Pylori. Briefly, tissues were deparaffinized and thermally treated with a citrate-based solution of antigenic retrieval (ImmunoDNA Retriever with Citrate, BIO SB, Santa Barbara, CA) using a water bath at 95°C for 1 hour. The IHC was conducted using a high sensiUvity Biotin- Streptavidin-HRP-DAB IHC system (ImmunoDetector HRP/DAB, BIO SB). The rnicroscopic observaUon evaluated the presence of mononuclear inflamrnatory celis and the identification of the bacteria in the wall or the vascular lumen. Results: Of the 34 cases studied 14 were positive, where one could identify the bacillus tu their different forms (41,17%) associated with chronic inflammation. Introducción: la infección y la inflamación crónica han sido implicadas como agentes etiológicos para la ateroesclerosis (ATE). Varios estudios han relacionado a la infección por H. Pylori (HP) con la EC, especialmente con los linajes mas virulentos (linaje Cag A). Objetivo: demostrar la presencia del HP en placas de endarterectomías, utilizando una técnica Inmunohistoquimica (IHQ) específica que revela una reacción Ag-Ac mediante un cromógeno. Material y Métodos: se estudiaron 34 placas ATE de distintos territorios vasculares. Se fijaron en formol, descalcificándolas en ácido fórmico según necesidad. Fueron incluidos en parafina, cortados y coloreados con H-E y técnicas de IHQ específicas para HP. Luego fueron desparafinados y tratados térmicamente con una solución de recuperación antigénica (Inmuno DNA Retriever with Citrate) utilizando olla a presión. La IHQ se efectuó con un sistema de alta sensibilidad Biotina-Estreptavidina- Peroxidasa- DAB). La observación morfológica evaluó células inflamatorias mononucleares y la identificación de la bacteria en la pared o la luz vascular. Resultados: de los 34 casos estudiados, en 14 se pudo identificar el bacilo en sus diferentes formas (41,17%), asociado a signos de inflamación crónica. Conclusión: el HP estuvo presente en un número sustancial de lesiones ATE y se asoció con inflamación. Estudios recientes sugieren que la presencia de HP demostrada por técnicas de IHQ, potenciaría los FR para ATE, induciendo una respuesta celular inflamatoria crónica por irritación persistente de la pared arterial. Universidad Nacional Córdoba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología 2019-11-15 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://revistas.unc.edu.ar/index.php/med/article/view/26423 Revista de la Facultad de Ciencias Médicas de Córdoba.; Vol. 63 No. 3 (2006); 17 - 23 Revista de la Facultad de Ciencias Médicas de Córdoba; Vol. 63 Núm. 3 (2006); 17 - 23 Revista da Faculdade de Ciências Médicas de Córdoba; v. 63 n. 3 (2006); 17 - 23 1853-0605 0014-6722 spa https://revistas.unc.edu.ar/index.php/med/article/view/26423/28191 Derechos de autor 2019 Universidad Nacional de Córdoba https://creativecommons.org/licenses/by-nc/4.0 |